Continuous preparation of bicelles using hydrodynamic focusing method for bicelle to vesicle transition

Choi, Sunghak; Kang, Byung Wook; Shimanouchi, Toshinori; Kim, Keesung; Jung, Ho‐Sup

doi:10.1186/s40486-021-00133-4

Cited by 7 publications

(3 citation statements)

References 19 publications

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“…Meanwhile, the liposome nanoformulation requires the vesiculation process, implying that the membrane stiffness has an influence on the size of liposome in addition to the nucleation aspect, which was examined through the addition of two additives: cholesterol and short-chain lipids. Cholesterol is widely used to enhance membrane rigidity, while short-chain lipids may potentially degrade membranes by de-aligning their lipids [33][34][35] . Here, liposomes containing cholesterol and short-chain lipids were synthesized hydrodynamically, and their membrane properties were measured via spectroscopic methods and then analyzed in relation to liposome size.…”

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confidence: 99%

Precise control of liposome size using characteristic time depends on solvent type and membrane properties

Choi

Kang

Yang

et al. 2023

Sci Rep

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Controlling the sizes of liposomes is critical in drug delivery systems because it directly influences their cellular uptake, transportation, and accumulation behavior. Although hydrodynamic focusing has frequently been employed when synthesizing nano-sized liposomes, little is known regarding how flow characteristics determine liposome formation. Here, various sizes of homogeneous liposomes (50–400 nm) were prepared according to flow rate ratios in two solvents, ethanol, and isopropyl alcohol (IPA). Relatively small liposomes formed in ethanol due to its low viscosity and high diffusivity, whereas larger, more poly-dispersed liposomes formed when using IPA as a solvent. This difference was investigated via numerical simulations using the characteristic time factor to predict the liposome size; this approach was also used to examine the flow characteristics inside the microfluidic channel. In case of the liposomes, the membrane rigidity also has a critical role in determining their size. The increased viscosity and packing density of the membrane by addition of cholesterol confirmed by fluorescence anisotropy and polarity lead to increase in liposome size (40–530 nm). However, the interposition of short-chain lipids de-aligned the bilayer membrane, leading to its degradation; this decreased the liposome size. Adding short-chain lipids linearly decreased the liposome size (130–230 nm), but at a shallower gradient than that of cholesterol. This analytical study expands the understanding of microfluidic environment in the liposome synthesis by offering design parameters and their relation to the size of liposomes.

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confidence: 99%

Precise control of liposome size using characteristic time depends on solvent type and membrane properties

Choi

Kang

Yang

et al. 2023

Sci Rep

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“…A follow‐up study explored the general utility of the microfluidic chip based on hydrodynamic focusing to produce bicelles composed of DHPC short‐chain phospholipid with other long‐chain phospholipids that have different gel‐to‐fluid phase transition temperatures ( T m ). [ 225 ] Interestingly, the effect of long‐chain phospholipid concentration on lipid nanoparticle morphology varied depending on the specific type of long‐chain phospholipid that was used in the microfluidic production process. While heterogeneous vesicles and bicelles were formed at low and high DMPC concentrations, respectively, replacing DMPC ( T m ≈ 23 °C) with 1‐palmitoyl‐2‐oleoyl‐ sn ‐glycero‐3‐phosphocholine (POPC; T m ≈ ‐2 °C) lipid led to a nearly opposite trend.…”

Section: Lipid Bicellesmentioning

confidence: 99%

Lipid Nanoparticle Technologies for Nucleic Acid Delivery: A Nanoarchitectonics Perspective

Ferhan

Park

et al. 2022

Adv Funct Materials

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Lipid-based nanoparticles have emerged as a clinically viable platform technology to deliver nucleic acids for a wide range of healthcare applications. Within this scope, one of the most exciting areas of recent progress and future innovation potential lies in the material science of lipid-based nanoparticles, both to refine existing nanoparticle strategies and to develop new ones. Herein, the latest efforts to develop next-generation lipid-based nanoparticles are covered by taking a nanoarchitectonics perspective and the design, nucleic acid encapsulation methods, scalable production, and application prospects are critically analyzed for three classes of lipid-based nanoparticles: 1) traditional lipid nanoparticles (LNPs); 2) lipoplexes; and 3) bicelles. Particular focus is placed on rationalizing how molecular self-assembly principles enable advanced functionalities along with comparing and contrasting the different nanoarchitectures. The current development status of each class of lipid-based nanoparticle is also evaluated and possible future directions in terms of overcoming clinical translation challenges and realizing new application opportunities are suggested.

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“…Meanwhile, the liposome nanoformulation requires the vesiculation process, implying that the membrane stiffness has an in uence on the size of liposome in addition to the nucleation aspect, which was examined through the addition of two additives: cholesterol and short-chain lipids. Cholesterol is widely used to enhance membrane rigidity, while short-chain lipids may potentially degrade membranes by dealigning their lipids [31][32][33] . Here, liposomes containing cholesterol and short-chain lipids were synthesized hydrodynamically, and their membrane properties were measured via spectroscopic methods and then analyzed in relation to liposome size.…”

Section: Introductionmentioning

confidence: 99%

Precise control of liposome size using characteristic time depends on solvent type and membrane properties

Choi

Kang

Yang

et al. 2022

Preprint

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Controlling the sizes of liposomes is critical in drug delivery systems because it directly influences their cellular uptake, transportation, and accumulation behavior. Although hydrodynamic focusing has frequently been employed when synthesizing nano-sized liposomes, little is known regarding how flow characteristics determine liposome formation. Here, various sizes of homogeneous liposomes (50–400 nm) were prepared according to flow rate ratios in two solvents, ethanol, and isopropyl alcohol (IPA). Relatively small liposomes formed in ethanol due to its low viscosity and high diffusivity, whereas larger, more poly-dispersed liposomes formed when using IPA as a solvent. This difference was investigated via numerical simulations using the characteristic time factor to predict the liposome size; this approach was also used to examine the flow characteristics inside the microfluidic channel. In case of the liposomes, the membrane rigidity also has a critical role in determining their size. The addition of cholesterol enhanced membrane properties such that the liposome size increased (40–530 nm). However, the interposition of short-chain lipids de-aligned the bilayer membrane, leading to its degradation; this decreased the liposome size. Adding short-chain lipids linearly decreased the liposome size (130–230 nm), but at a shallower gradient than that of cholesterol. This analytical study expands the understanding of microfluidic environment in the liposome synthesis by offering design parameters and their relation to the size of liposomes.

show abstract

Continuous preparation of bicelles using hydrodynamic focusing method for bicelle to vesicle transition

Cited by 7 publications

References 19 publications

Precise control of liposome size using characteristic time depends on solvent type and membrane properties

Precise control of liposome size using characteristic time depends on solvent type and membrane properties

Lipid Nanoparticle Technologies for Nucleic Acid Delivery: A Nanoarchitectonics Perspective

Precise control of liposome size using characteristic time depends on solvent type and membrane properties

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