Continuous production and recovery of itaconic acid in a membrane bioreactor

Emerging biotechnologies for production of itaconic acid and its applications as a platform chemical

“…ED with bipolar membrane has the potential for continuous recovery of IA [84]. Carstensen et al [12] investigated continuous production and recovery of IA in a reverse-flow diafiltration membrane reactor in laboratory scale using glucose (50 g L −1 ) and U. maydis MB 215. The fermentation was run at controlled pH 6.0 and 30 °C.…”

Metabolic engineering of Corynebacterium glutamicum for the production of itaconate

“…These included e.g. the optimization of production with A. terreus (Hevekerl et al, 2014;Kuenz et al, 2012;Tevz et al, 2010), the development of processes with other natural itaconate producers such as Ustilago maydis (Carstensen et al, 2013;Klement et al, 2012;Maassen et al, 2014;Panakova et al, 2009;Voll et al, 2012), or the use of Aspergillus niger as production host Li et al, 2011Li et al, , 2012Li et al, , 2013van der Straat et al, 2013van der Straat et al, , 2014. A. niger is a highly efficient citric acid producer, reaching titers above 200 g l À 1 , but does not naturally produce itaconic acid and thus requires heterologous genes, e.g.…”

New approaches for itaconic acid production: bottlenecks and possible remedies

“…Cell retention can be accomplished by immobilization of microorganisms in membranes [91]. The use of a continuous membrane bioreactor with U. maydis was found to give IA production of 0.8 g L À1 h À1 [92]. The major disadvantage of the membrane processes is cake layer formation, which can eventually lead to performance deterioration and membrane fouling.…”