Continuous production of biohydrogen from brewery effluent using co-culture of mutated Rhodobacter M 19 and Enterobacter aerogenes

Veeramalini, J.B.; Selvakumari, I. Aberna Ebenezer; Park, Sungkwon; Jayamuthunagai, J.; Bharathiraja, B.

doi:10.1016/j.biortech.2019.121402

Cited by 34 publications

(10 citation statements)

References 35 publications

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“…An optimal H 2 yield of 2877 mL H 2 was achieved using immobilized ethidium bromide-mutated co-cultures [78]. The immobilized ethidium bromide-mutated co-cultures also enhanced the COD removal by 85% [78]. Furthermore, it has been shown that cell immobilization improves the activity of the predominant H 2 -producers such as Clostridium, Bacillus, and Enterobacter species [79][80][81][82].…”

Section: Cell Immobilizationmentioning

confidence: 98%

“…The use of entrapped biocatalysts increased the concentration of cells within the medium, resulting in high glucose consumption [77]. In another recent study, immobilized mutated (with ethidium bromide and ultraviolet) co-cultures of Rhodobacter M19 and Enterobacter aerogenes were studied in DF using brewery effluent [78]. An optimal H 2 yield of 2877 mL H 2 was achieved using immobilized ethidium bromide-mutated co-cultures [78].…”

Section: Cell Immobilizationmentioning

confidence: 99%

“…In another recent study, immobilized mutated (with ethidium bromide and ultraviolet) co-cultures of Rhodobacter M19 and Enterobacter aerogenes were studied in DF using brewery effluent [78]. An optimal H 2 yield of 2877 mL H 2 was achieved using immobilized ethidium bromide-mutated co-cultures [78]. The immobilized ethidium bromide-mutated co-cultures also enhanced the COD removal by 85% [78].…”

Section: Cell Immobilizationmentioning

confidence: 99%

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Revising the dark fermentative H2 research and development scenario – An overview of the recent advances and emerging technological approaches

Sekoai

Daramola

Mogwase

et al. 2020

Biomass and Bioenergy

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The indiscriminate use of fossil fuels has led to several challenges such as greenhouse gas emissions, environmental degradation, and energy security. Establishment of clean fuels is at the forefront of science and innovation in today's society to curb these problems. Dark fermentation (DF) is widely regarded as the most promising clean energy technology of the 21st century due to its desirable properties such as high energy content, its non-polluting features, its ability to use a broad spectrum of feedstocks and inoculum sources, as well as its ability to use mild fermentation conditions. In developing nations, this technology could be instrumental in establishing effective waste disposal systems while boosting the production of clean fuels. However, DF is still hindered by the low yields which stagnate its commercialization. This paper reviews the recent and emerging technologies that are gaining prominence in DF based on information that has been gathered from recent scientific publications. Herein, novel enhancement methods such as cell immobilization, nanotechnology, mathematical optimization tools, and technologies for biogas upgrading using renewable H 2 are comprehensively discussed. Furthermore, a section which discusses the potential of bioenergy in Sub-Saharan Africa including South Africa is included. Finally, scientific areas that need further research and development in DF process are also presented.

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Section: Cell Immobilizationmentioning

confidence: 98%

Section: Cell Immobilizationmentioning

confidence: 99%

Section: Cell Immobilizationmentioning

confidence: 99%

See 1 more Smart Citation

Revising the dark fermentative H2 research and development scenario – An overview of the recent advances and emerging technological approaches

Sekoai

Daramola

Mogwase

et al. 2020

Biomass and Bioenergy

View full text Add to dashboard Cite

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“…Acetic acid consumption by R. toruloides is an important characteristic of this yeast, since this carbon source is commonly present in hemicellulosic hydrolysates. Moreover, studies have tested the potential use of acetic acid co-generated during anaerobic treatment of wastewater as a carbon source for lipid production by yeasts [37,38]. Therefore, this acid is a potential source of cheap carbon for lipid production, since wastewater treatment by anaerobic bacteria is widely used by many industries [39].…”

Section: R Toruloides Cultivation and Lipid Productionmentioning

confidence: 99%

Rhodotorula toruloides Single Cell Oil Production Using Eucalyptus urograndis Hemicellulose Hydrolysate as a Carbon Source

2020

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Microbial oil is a potential substitute for vegetable oils in the biodiesel industry. Efforts to obtain cheap carbon sources for the cultivation of lipid-producing microorganisms comprise an active research area. This work aimed to extract the hemicellulose fraction from Eucalyptus uograndis and to use its hydrolysate as a carbon source for Rhodotorula toruloides (an oleaginous yeast) cultivation for microbial oil production. Hemicellulose hydrothermal extractions were performed at different temperatures, times, and ratios of solid to liquid (S/L). Temperature and time showed a stronger effect on the solubilization of hemicellulose. Hemicellulose extraction at 155 °C, 195 min, and an S/L ratio of 1/2 resulted in a hydrolysate with a xylose content of 37.0 g/l. R. toruloides cultivation in this hydrolysate showed that initial pH had a strong influence on cell growth. At an initial pH of 6.2, cells grew to 6.0 g/l of biomass with a lipid content of 50%. Therefore, we believe that E. urograndis hemicellulose hydrolysate could be a potential substrate for R. toruloides for lipid production based on the biorefinery concept.

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“…Various biomass and renewable substrates are considered suitable substrate for biohydrogen production due to their high organic richness, less nutrient need, and positive net energy production. In particular, industrial wastewaters are abundantly available substrates, and they are categorized as low-cost and extensively obtainable biodegradable substrates [5,6,7]. Using waste as a substrate than others is economical, and it can reduce the accumulation of waste in the environment.…”

Section: Introductionmentioning

confidence: 99%

Biohydrogen Production in Substrates Combination of Vinasse and Tofu Whey Using Photosynthetic Bacteria Rhodobium marinum

Nusaibah¹,

Syamsu²,

Susilaningsih³

2020

Int. J. Adv. Sci. Eng. Inf. Technol.

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Hydrogen gas is generated by the fossil fuel refinery process, fixation of sunlight, wind turbine, and water electrolysis. Hydrogen gas is promising energy due to its high energy content and clean combustion. It is also able to be produced by fermentation series, using waste as a substrate. Vinasse is a waste of ethanol distillation from fermented molasses, which still has high Chemical Oxygen Demand (COD) content. The COD is mainly composed of many organic loads that potential to produce hydrogen. Tofu whey (TW), which still has high nitrogen content, is waste generated from the tofu production process. TW is reported to contain high of valuable nutrients, including protein that contains nitrogen. The combination of both liquid wastes is a suitable substrate for hydrogen gas production using Rhodobium marinum. This work aimed to study hydrogen gas, Hydrogen Production Rate, and COD Removal Rate of hydrogen production using a combination of both liquid wastes. Biohydrogen production was examined by varying COD of vinasse/Nitrogen from TW (10,000/1-50,000/1) at a particular fermentation time in the 3rd, 6th, and 9th days. The highest hydrogen gas and Hydrogen Production Rate (HPR) was obtained at substrate concentration 40,000/1 on 9th day of fermentation, namely 95.72±6.51 mL H2/L and 121.44 mL H2/L/d, respectively. COD removal and COD Removal Rate were 7820±400.69 mg COD/L and 799.77 mg COD/L/d. Thus, it can be concluded that the combination of Vinasse and TW has potential as a substrate to biohydrogen production using Rhodobium marinum.

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Continuous production of biohydrogen from brewery effluent using co-culture of mutated Rhodobacter M 19 and Enterobacter aerogenes

Cited by 34 publications

References 35 publications

Revising the dark fermentative H2 research and development scenario – An overview of the recent advances and emerging technological approaches

Revising the dark fermentative H2 research and development scenario – An overview of the recent advances and emerging technological approaches

Rhodotorula toruloides Single Cell Oil Production Using Eucalyptus urograndis Hemicellulose Hydrolysate as a Carbon Source

Biohydrogen Production in Substrates Combination of Vinasse and Tofu Whey Using Photosynthetic Bacteria Rhodobium marinum

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