Continuous Production of Lipase‐Catalyzed Biodiesel in  a Packed‐Bed Reactor: Optimization and Enzyme Reuse Study

Chen, Hsiao‐Ching; Ju, Hen-Yi; Wu, Tsung-Ta; Liu, Yung-Chuan; Lee, Chih-Chen; Chang, Cheng‐Nan; Chung, Yi-Lin; Shieh, Chwen‐Jen

doi:10.1155/2011/950725

Cited by 59 publications

(30 citation statements)

References 27 publications

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“…Therefore, there is a current demand to find out the alternative eco-friendly fuel against petrodiesel. Biodiesel has been considered as a major alternative for fossil fuel, as it is a biodegradable, renewable and nontoxic fuel [2]. Fatty acid methyl esters originating from vegetable oils and animal fats are known as biodiesel.…”

Section: Introductionmentioning

confidence: 99%

Effect of Various Pretreatment for Extracting Intracellular Lipid fromNannochloropsis oculataunder Nitrogen Replete and Depleted Conditions

Surendhiran

Mani

2014

ISRN Chemical Engineering

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Microalga is one of the most compelling microbial biomasses for biodiesel production. Various pretreatment processes, namely, enzyme treatment, lysis by acid, ultrasonicator, microwaves, autoclave, and 40% NaCl, for nitrogen replete and depleted algal cultures of Nannochloropsis oculata had been carried out to check the most feasible and effective technique to disrupt cells for procuring lipids, for which concentrations were determined. Fatty acid composition, essential functional groups, and cell disruption were analyzed by GC-MS, FT-IR Spectroscopy, and Nile Red fluorescent microscopy, respectively. The present investigation showed that lipid yield was higher in nitrogen depleted cells than that in normally nourished cells. GC-MS revealed the presence of major fatty acids-palmitic, oleic, stearic, arachidic, lauric, and linoleic acids. Highest efficiency was found when cells were pretreated using acid for 3 h. The lipid content was calculated as 33.18% and 54.26% for nitrogen rich cells and nitrogen starved cells, respectively. This work thus aided in identifying the most eligible pretreatment process to avail lipids from cells, to convert them to eco-friendly and nonpolluting biodiesel.

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Section: Introductionmentioning

confidence: 99%

Effect of Various Pretreatment for Extracting Intracellular Lipid fromNannochloropsis oculataunder Nitrogen Replete and Depleted Conditions

Surendhiran

Mani

2014

ISRN Chemical Engineering

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“…Smaller capacities facilitate interactions between the substrate, lipase and alcohol. The reaction yield has been tested in packed bed reactors [44][45][46], showing a higher efficiency when the reaction media has low flows through the stationary phase, increasing the contact between catalyst and reactants. Likewise, the enzyme amount influences the yield and the rate of the reaction, due to increased number of enzyme molecules able to react with the substrate [18,22], as explained by Murray et al [42].…”

Section: Discussionmentioning

confidence: 99%

Applied Biocatalysis with an Organic Resistant Partially Purified Lipase from P. aeruginosa During FAME Production

Cabrejo¹,

Barrera²,

Prieto-Correa³

2015

TOCATJ

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A partially purified lipase from the Pseudomonas aeruginosa strain (PSA-01) isolated from the palm oil fruit Elaeis guineensis was used as biocatalyst to produce fatty acid methyl esters (FAME). Lyophilized lipase supernatant (LLS) was used during the first step to screen the main variables (pH, temperature, stoichiometric oil:methanol ratio, water content and type of oil). Other variables which were identified during the screening assays (scale of reaction recipient, LLS amount and use of hexane to solubilize methanol forming a methanol-oil microemulsion) were tested during a second step. The response variable was % molar yield of FAME. It was quantified by GC. Additionally, the LLS work parameters were optimized and compared to a partially purified lipase (PPL) during a final assay. The first-order interactions between the analyzed factors were significant (p<0.05). The highest yield was 4.16% w/w (respect to oil) using a partially purified lipase (PPL) with pH 8, refined, bleached, and deodorized oil (RBD), 5% water (by volume) in oil and 10% hexane (by volume), and a stoichiometric ratio of 1:170 oil:methanol. The final assay was carried out at 54°C and 200 rpm for 48 hours. It resulted in a 34.68% conversion using PPL. It also showed a 13-fold improvement versus the initial yield with LLS, suggesting the need for a better purification process. During this research, the lipase was partially purified and used at an alkaline pH. It showed resistance to organic compounds such as methanol and hexane. This implies great potential to act as an effective biocatalyst in the implementation of biodiesel production processes.

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“…The alkaline and enzymatic catalyst concentrations of 1.5 and 4% by weight of oil, respectively, were used considering yields from 95 to 98% conversion of oil into esters [19,20]. It was also accounted the enzyme reuse, considering that immobilized enzyme operates over 30 days without losing catalytic activity [21]. Oil content and molar weight of pequi oil were assumed to be 50% and 1,171.1 g mol-1 [22,23].…”

Section: Methodsmentioning

confidence: 99%

Preliminary Economic Study of Biodiesel Production from Pequi in Brazil

Neto¹

2017

OAJS

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This study deals with the economic feasibility of biodiesel production in Brazil by using the pequi oil as raw matter. The software SIMB-E was used during simulations in which a cash flow model related economic indexes with biodiesel production features. Competitiveness between selling prices of biodiesel and petro diesel, fixed capital costs, operating costs, depreciation, and auxiliary costs were also considered during the economic calculations. A total of 8 scenarios of production were simulated and 4 of them presented competitive biodiesel selling prices, being considered as worthwhile projects in the final analysis. But, all simulated scenarios were found to be potentially profitable and should be motivated considering sustainability, social and environmental aspects. The worthwhile projects were plants with alkaline transesterification, indicating that the success of biodiesel production from alternative raw matter still depends on incomes from the feedstock co-products and glycerol, as well as on the costs of catalyst and feedstock. Pequi contributed to the feasibility of biodiesel production due to its high yield, low costs of fruits and good valuation of its co-products.

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Continuous Production of Lipase‐Catalyzed Biodiesel in a Packed‐Bed Reactor: Optimization and Enzyme Reuse Study

Cited by 59 publications

References 27 publications

Effect of Various Pretreatment for Extracting Intracellular Lipid fromNannochloropsis oculataunder Nitrogen Replete and Depleted Conditions

Effect of Various Pretreatment for Extracting Intracellular Lipid fromNannochloropsis oculataunder Nitrogen Replete and Depleted Conditions

Applied Biocatalysis with an Organic Resistant Partially Purified Lipase from P. aeruginosa During FAME Production

Preliminary Economic Study of Biodiesel Production from Pequi in Brazil

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