Continuous production of monoclonal mouse IgE antibodies with known allergenic specificity by a hybrid cell line

Böttcher, I.; Hämmerling, G. J.; Kapp, Joachim‐Friedrich

doi:10.1038/275761a0

Cited by 56 publications

(19 citation statements)

References 9 publications

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“…In one experi ment, ascites fluid from mice bearing a growing IgE-producing hybridoma [8], with specificity against OA (kindly provided by Dr. A. H. Rose) was also employed -the fluid exhibited an anti-OA PCA-antibody title of 2,560, and was neg ative by HA. In these studies, all recipients were inoculated i.v.…”

Section: Serum In Adoptive Transfermentioning

confidence: 99%

Adoptive Transfer of ‘Persistent’ IgE Responses in Mice in the Absence of Secondary Antigenic Stimulation

Holt¹,

Leivers²,

Batty³

1981

Int Arch Allergy Immunol

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Spleen cells were prepared from Balb/c mice immunized 30 days previously with alum-precipitated ovalbumin (OA), which manifested high, persistent titres of anti-OA IgE and IgG. The adoptive transfer of 5.0 × 10⁷ such cells to X-irradiated syngeneic recipients produced comparable persistent IgE/IgG responses, in the absence of secondary antigenic challenge. Fractionation procedures indicated that the nylon-wool adherent population from the spleen was the most active in effecting transfer of the response. However, donor T-cells were also required, as pretreatment of the cellular inoculum with anti-Thy 1.2 antiserum ablated transfer. The inclusion of serum containing anti-OA IgG (but not IgE) in the cellular inoculum also blocked the transfer.

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Section: Serum In Adoptive Transfermentioning

confidence: 99%

Adoptive Transfer of ‘Persistent’ IgE Responses in Mice in the Absence of Secondary Antigenic Stimulation

Holt¹,

Leivers²,

Batty³

1981

Int Arch Allergy Immunol

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“…In addition, RAST for penicil loyl was employed using benzylpenicilloyl human serum albumin-conjugated paper discs (Pharmacia Ltd., Uppsala, Sweden). Rabbit antibodies against mouse IgE [8] had been chromatographed on partly purified mouse hybridoma IgE (IgE-14-205) [3] and purified by absorption with fetal calf serum and nor mal mouse serum as well as by isolation from a rat myeloma IgE (IR2) column [7,8]. The eluted antibod ies were dialyzed, concentrated and l25I-labelled by the chloramine T procedure [5].…”

mentioning

confidence: 99%

Boosterable IgE Antibody Response in Mice without the Use of Adjuvant

Hall¹,

Ahlstedt²,

Kristofferson³

1982

Int Arch Allergy Immunol

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A mouse model is presented giving boosterable IgE antibody responses preceding the IgG antibody responses without using adjuvants. Groups of CBA mice were injected subcutaneously with minute doses of penicilloyl bovine gammaglobulin, 3.8–94 ng daily for one or two 10-day periods. Some groups of animals were simultaneously given Bordetella pertussis bacteria intraperitoneally. The IgE antibody responses were recorded with passive cutaneous anaphylaxis (PCA) in rats as well with RAST. IgG antibody responses were determined with a double antibody assay. An overall good agreement between the antibody activity in the PCA and RAST was seen, both tests showing boosterable IgE antibody responses. Some discrepancies between the two tests are discussed.

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“…Monoclonal Antibodies (I) IgE-14-205 (IgE-OA) with ovalbumin (OA) specificity [3], (2) IgE-53-569 (IgE-DNP) with DNP specificity. The two hybridoma-derived murine IgE antibodies have been shown to be typical murine reaginic antibodies [3,4], The monoclonal murine lgG r DNP antibody has been produced by cell fusion as described [4], The hapten and class specificity of this antibody has been esti mated by radioimmunodiffusion with DNP-,5S-methionine and class-specific antimouse antisera [un published].…”

Section: Methodsmentioning

confidence: 99%

Direct Evidence for the Heterogeneity of the IgE and the IgG<sub>1</sub> Receptor on Murine Mast Cells

Böttcher¹,

Albring²

1981

Int Arch Allergy Immunol

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Continuous production of monoclonal mouse IgE antibodies with known allergenic specificity by a hybrid cell line

Cited by 56 publications

References 9 publications

Adoptive Transfer of ‘Persistent’ IgE Responses in Mice in the Absence of Secondary Antigenic Stimulation

Adoptive Transfer of ‘Persistent’ IgE Responses in Mice in the Absence of Secondary Antigenic Stimulation

Boosterable IgE Antibody Response in Mice without the Use of Adjuvant

Direct Evidence for the Heterogeneity of the IgE and the IgG<sub>1</sub> Receptor on Murine Mast Cells

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