Continuous production of selenomethionine-enriched Chlorella sorokiniana biomass in a photobioreactor

Gojkovic, Živan; Garbayo-Nores, Inés; Gómez-Jacinto, Veronica; García-Barrera, Tamara; Gómez-Ariza, José Luis; Márová, Ivana; Vílchez, Carlos

doi:10.1016/j.procbio.2013.06.013

Cited by 14 publications

(9 citation statements)

References 26 publications

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“…During the first 24 h of cultivation, values of Y op for Se-exposed and control cultures decreased in approximately 15 and 10%, respectively, compared to initial values, due to culture adaptation phase. Nevertheless, this decrease was only temporary and Y op values stabilized after 24 h, as previously found [32]. …”

Section: Resultssupporting

confidence: 83%

“…Related studies on Scenedesmus quadricauda revealed that 50 mg·L −1 selenate in medium was not lethal to microalga cultures as cells grew and divided normally [ 28 ]. In our case, a selenate concentration of 40 mg·L −1 (212 μ M) in the culture medium was selected based on previously published data [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

“…Similar studies have been done with Chlorella zofingiensis with emphasis on heat-stable selenoproteins [ 30 ], on metabolism of Se volatile compounds [ 14 ], Se effect on Chlorella sp. cultivated on glucose [ 16 ], and Se effect on continuous microalgae cultures of Chlorella pyrenoidosa [ 31 ] and Chlorella sorokiniana [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Selenate on Viability and Selenomethionine Accumulation ofChlorella sorokinianaGrown in Batch Culture

Gojkovic

Vílchez

Torronteras

et al. 2014

The Scientific World Journal

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The aim of this work was to study the effect of Se(+VI) on viability, cell morphology, and selenomethionine accumulation of the green alga Chlorella sorokiniana grown in batch cultures. Culture exposed to sublethal Se concentrations of 40 mg·L−1 (212 μM) decreased growth rates for about 25% compared to control. A selenate EC50 value of 45 mg·L−1 (238.2 μM) was determined. Results showed that chlorophyll and carotenoids contents were not affected by Se exposure, while oxygen evolution decreased by half. Ultrastructural studies revealed granular stroma, fingerprint-like appearance of thylakoids which did not compromise cell activity. Unlike control cultures, SDS PAGE electrophoresis of crude extracts from selenate-exposed cell cultures revealed appearance of a protein band identified as 53 kDa Rubisco large subunit of Chlorella sorokiniana, suggesting that selenate affects expression of the corresponding chloroplast gene as this subunit is encoded in the chloroplast DNA. Results revealed that the microalga was able to accumulate up to 140 mg·kg−1 of SeMet in 120 h of cultivation. This paper shows that Chlorella sorokiniana biomass can be enriched in the high value aminoacid SeMet in batch cultures, while keeping photochemical viability and carbon dioxide fixation activity intact, if exposed to suitable sublethal concentrations of Se.

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Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 99%

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Effect of Selenate on Viability and Selenomethionine Accumulation ofChlorella sorokinianaGrown in Batch Culture

Gojkovic

Vílchez

Torronteras

et al. 2014

The Scientific World Journal

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“…For the first determination, a peak is observed which contains the maximum nitrate absorption plus the so-called interference, whereas for the second one, only the effect of turbidity is considered. Accordingly, one can compute: (16) where N OD corresponds to the nitrate concentration from the optical density measurement. A Shimadzu ® UVmini-1240 spectrophotometer is used, with a sensitivity of ±0.1 for absorbance measurements.…”

Section: Substrate Measurementmentioning

confidence: 99%

“…For the production of microalgae in photobioreactors, the chemostat mode, i.e., equal inlet and outlet flows seems to be more profitable, because it allows prolonged stable operation, possibly at maximum productivity [16]. Microalgae photobioreactors are complex systems, mainly due to their nonlinear and time-varying behavior.…”

Section: Introductionmentioning

confidence: 99%

Experimental Study of Substrate Limitation and Light Acclimation in Cultures of the Microalgae Scenedesmus obliquus—Parameter Identification and Model Predictive Control

et al. 2020

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In this study, the parameters of a dynamic model of cultures of the microalgae Scenedesmus obliquus are estimated from datasets collected in batch photobioreactors operated with various initial conditions and light illumination conditions. Measurements of biomass, nitrogen quota, bulk substrate concentration, as well as chlorophyll concentration are achieved, which allow the determination of parameters with satisfactory confidence intervals and model cross-validation against independent data. The dynamic model is then used as a predictor in a nonlinear model predictive control strategy where the dilution rate and the incident light intensity are simultaneously manipulated in order to optimize the cumulated algal biomass production.

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Photobioreactors for Wastewater Treatment

Ashok

Gupta

Shriwastav

2019

Application of Microalgae in Wastewater Treatment

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Continuous production of selenomethionine-enriched Chlorella sorokiniana biomass in a photobioreactor

Cited by 14 publications

References 26 publications

Effect of Selenate on Viability and Selenomethionine Accumulation ofChlorella sorokinianaGrown in Batch Culture

Effect of Selenate on Viability and Selenomethionine Accumulation ofChlorella sorokinianaGrown in Batch Culture

Experimental Study of Substrate Limitation and Light Acclimation in Cultures of the Microalgae Scenedesmus obliquus—Parameter Identification and Model Predictive Control

Photobioreactors for Wastewater Treatment

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