Continuous purification of influenza virus using simulated moving bed chromatography

Adapting viral safety assurance strategies to continuous processing of biological products

“…The counter‐current flow which allows for more efficient separation is ideally designed for adaption to continuous downstream clarification/polishing processes. Membrane adsorbers in SMB technology systems have already been proven in influenza virus purification (Krober et al, ), protein purifications (Hardick et al, ), and theoretically will decrease downstream processing time by 50% and process operating expenses by 23% (Varadaraju et al, ).…”

Adapting viral safety assurance strategies to continuous processing of biological products

“…The counter-current flow which allows for more efficient separation is ideally designed for adaption to continuous downstream clarification/polishing processes. Membrane adsorbers in SMB technology systems have already been proven in influenza virus purification (Krober et al, 2013), protein purifications (Hardick et al, 2015), and theoretically will decrease downstream processing time by 50% and process operating expenses by 23% (Varadaraju et al, 2011).…”

Polysaccharide-based chromatographic adsorbents for virus purification and viral clearance

“…To date, biopharmaceuticals targeted by SMB chromatography (SMBC) are mainly proteins and mAbs. Nevertheless, SEC-SMBC has been recently applied to purification of cell culture-derived IAV [34] and human AdV type 5 (AdV-5) [35] using columns packed with Seph 4 FF resin. Particularly promising results were obtained by Nestola et al [35] with a two-column setup yielding higher virus recovery and purity (HC DNA and protein clearance) than the batch (single-column) SEC process with the same amount of stationary phase.…”