Continuous, rapid biological aerosol detection with the use of UV fluorescence: Outdoor test results

Eversole, Jay D.; Hardgrove, J.; Cary, W. K.; Choulas, D. P.; Seaver, Mark

doi:10.1002/(sici)1520-6521(1999)3:4/5<249::aid-fact4>3.0.co;2-o

Cited by 42 publications

(20 citation statements)

References 9 publications

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“…BAWS extracts information from the scattering particle size and fluorescence intensities in the UV (300-400 nm) and visible bands (400-600 nm), and can discriminate bioaerosols particles from other non-bioaerosol particles with higher specificity than UV-APS. Most of the commercialized or laboratory instruments based on one or two broad fluorescence bands using one wavelength excitation have some degree of discrimination ability similar to UV-APS and BAWS (e.g., [77,27,83,93,19,41]). …”

Section: Fluorescent Molecules and Their Excitation-emission Matrix (mentioning

confidence: 99%

Detection and characterization of biological and other organic-carbon aerosol particles in atmosphere using fluorescence

Pan

2015

Journal of Quantitative Spectroscopy and Radiative Transfer

121

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a b s t r a c tThis paper offers a brief review on the detection and characterization of biological and other organic-carbon (OC) aerosol particles in atmosphere using laser-induced-fluorescence (LIF) signatures. It focuses on single individual particles or aggregates in the micron and supermicron size range when they are successively drawn through the interrogation volume of a point detection system. Related technologies for these systems that have been developed in last two decades are also discussed. These results should provide a complementary view for studying atmospheric aerosol particles, particularly bioaerosol and OC aerosol particles from other analytical technologies.Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

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Section: Fluorescent Molecules and Their Excitation-emission Matrix (mentioning

confidence: 99%

Detection and characterization of biological and other organic-carbon aerosol particles in atmosphere using fluorescence

Pan

2015

Journal of Quantitative Spectroscopy and Radiative Transfer

121

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“…Instrumentation available for military research use, but not commercial/civilian use, include aerosol fluorescence spectrum analyzers (AFSA) (Pinnick et al, 1995(Pinnick et al, , 2004Pan et al, 2011) and single-particle fluorescence analyzers (SPFA) (Eversole et al, 1999;Sivaprakasam et al, 2011). Within approximately the last decade, commercially available devices have become obtainable for purchase and use by non-military communities.…”

Section: A Healy Et Al: Ambient Measurements Of Biological Aerosmentioning

confidence: 99%

Ambient measurements of biological aerosol particles near Killarney, Ireland: a comparison between real-time fluorescence and microscopy techniques

et al. 2014

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Abstract. Primary biological aerosol particles (PBAPs) can contribute significantly to the coarse particle burden in many environments. PBAPs can thus influence climate and precipitation systems as cloud nuclei and can spread disease to humans, animals, and plants. Measurement data and techniques for PBAPs in natural environments at high time-and size resolution are, however, sparse, and so large uncertainties remain in the role that biological particles play in the Earth system. In this study two commercial real-time fluorescence particle sensors and a Sporewatch single-stage particle impactor were operated continuously from 2 August to 2 September 2010 at a rural sampling location in Killarney National Park in southwestern Ireland. A cascade impactor was operated periodically to collect size-resolved particles during exemplary periods. Here we report the first ambient comparison of a waveband integrated bioaerosol sensor (WIBS-4) with a ultraviolet aerodynamic particle sizer (UV-APS) and also compare these real-time fluorescence techniques with results of fluorescence and optical microscopy of impacted samples. Both real-time instruments showed qualitatively similar behavior, with increased fluorescent bioparticle concentrations at night, when relative humidity was highest and temperature was lowest.The fluorescent particle number from the FL3 channel of the WIBS-4 and from the UV-APS were strongly correlated and dominated by a 3 µm mode in the particle size distribution. The WIBS FL2 channel exhibited particle modes at approx. 1 and 3 µm, and each was correlated with the concentration of fungal spores commonly observed in air samples collected at the site (ascospores, basidiospores, Ganoderma spp.). The WIBS FL1 channel exhibited variable multimodal distributions turning into a broad featureless single mode after averaging, and exhibited poor correlation with fungal spore concentrations, which may be due to the detection of bacterial and non-biological fluorescent particles. Cladosporium spp., which are among the most abundant fungal spores in many terrestrial environments, were not correlated with any of the real-time fluorescence channels, suggesting that the real-time fluorescence instruments are relatively insensitive to PBAP classes with dark, highly absorptive cell walls.Fluorescence microscopy images of cascade impactor plates showed large numbers of coarse-mode particles consistent with the morphology and weak fluorescence expected of sea salt. Some of these particles were attached to biological cells, suggesting that a marine source influenced the PBAPs observed at the site and that the ocean may be an important contributor to PBAP loadings in coastal environments.

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“…Figure 8 shows an example of data collected outdoors by the Single Particle Fluorescence Analyzer (SPFA), a developmental detector at the Naval Research Laboratory (NRL), Washington, DC. 9 ' 10,11 The SPFA counts and sizes all entering particles using a cw laser for optical scattering. In addition, it is designed to differentiate particles of biological origin (also known as bioaerosols) from all other aerosols.…”

Section: Discussionmentioning

confidence: 99%

Background Aerosols and BW Detection: Problems and Suggested Solutions

Birenzvige¹,

Wick²

2002

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Continuous, rapid biological aerosol detection with the use of UV fluorescence: Outdoor test results

Cited by 42 publications

References 9 publications

Detection and characterization of biological and other organic-carbon aerosol particles in atmosphere using fluorescence

Detection and characterization of biological and other organic-carbon aerosol particles in atmosphere using fluorescence

Ambient measurements of biological aerosol particles near Killarney, Ireland: a comparison between real-time fluorescence and microscopy techniques

Background Aerosols and BW Detection: Problems and Suggested Solutions

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