Continuous sheathless microparticle and cell patterning using CL-SSAWs (conductive liquid-based standing surface acoustic waves)

Nam, Jeonghun; Kim, Jae Young; Lim, Chae Seung

doi:10.1063/1.4975397

Cited by 10 publications

(7 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For N B = 1:10 6 where the infected cells were difficult to be found, we realized high enrichment (≈3260), which enhanced the detection of the infected cells. In fact, we were able to find the infected cells from each thin blood smear of the sample taken from the outlet D. The throughput obtained in this work (2.5 × 10 5 cells/min) is higher when compared with those in references [32,33] but lower than those in references [31,35], which treated a different number ratios of the target cells to the normal cells. Finally, it should be noted that due to the simplicity of the device structure and operation, the proposed device can readily be operated in parallel.…”

Section: Infected Cell Separationmentioning

confidence: 68%

A discrete dielectrophoresis device for the separation of malaria‐infected cells

et al. 2022

View full text Add to dashboard Cite

Malaria is a serious disease caused by Plasmodium parasites that infect red blood cells (RBCs). This paper presents the continuous separation of malaria‐infected RBCs (iRBCs) from normal blood cells. The proposed method employed the discrete dielectrophoresis (DEP) in a microfluidic device with interdigitated electrodes. Our aim is to treat a sample having high concentration of cells to realize high throughput and to prevent the clogging of the microchannel with the use of the discrete DEP. The discrete DEP force for deflecting cells in the device was controlled by adjusting the magnitude, frequency, and duty cycle of the applied voltage. The effectiveness of the proposed method was demonstrated by separating the malaria‐infected cells in samples having a cell concentration of 106 cells/µl. From experimental results, we determined the enrichment that is needed to enhance the detection in the case of low parasitemia. The enrichment of the infected cells at the device output was 3000 times as high as that of the input containing 1 infected cell to 106 normal cells. Therefore, the proposed method is highly effective and can significantly facilitate the detection of the infected cells for the identification of Malaria patients.

show abstract

Section: Infected Cell Separationmentioning

confidence: 68%

A discrete dielectrophoresis device for the separation of malaria‐infected cells

et al. 2022

View full text Add to dashboard Cite

show abstract

“…However, these techniques are dependent upon the electrical and magnetic properties of the particles and the suspension media, which limits their applicability. [12][13][14] Acoustic forces have been used to manipulate (align, [15][16][17] pattern, [18][19][20] separate, [21][22][23] and concentrate [24][25][26] ) microparticles based on their size differences and mechanical properties. Acoustic waves are suitable for the handling of virtually any microobject and are not dependent on the electro-magnetic properties of the material and the suspension media.…”

Section: Introductionmentioning

confidence: 99%

Microparticle self-assembly induced by travelling surface acoustic waves

et al. 2019

View full text Add to dashboard Cite

We present an acoustofluidic method based on travelling surface acoustic waves (TSAWs) for the induction of the selfassembly of microparticles inside a microfluidic channel. The particles are trapped above an interdigitated transducer, placed directly beneath the microchannel, by the TSAW-based direct acoustic radiation force (ARF). This approach was applied to 10 μm polystyrene particles, which were pushed towards the ceiling of the microchannel by 72 MHz TSAWs to form singleand multiple-layer colloidal structures. The repair of cracks and defects within the crystal lattice occurs as part of the self-assembly process. The sample flow through the first inlet can be switched with a buffer flow through a second inlet to control the number of particles in the crystalline structure. The constant flow-induced Stokes drag force on the parti-cles is balanced by the opposing TSAW-based ARF. This force balance is essential for the acoustics-based self-assembly of microparticles inside the microchannel. Moreover, we studied the effects of varying the input voltage and fluid flow rate on the position and shape of the colloidal structure. The active self-assembly of microparticles into crystals with multiple layers can be used in the bottomup fabrication of colloidal structures with dimensions greater than 500 μm x 500 μm, which is expected to have important applications in various fields.

show abstract

“…The system parameters such as microchannel width, position of the IDTs pairs and acoustic signal wavelength are set to generate one pressure node at the center of the microchannel in the alignment stage, and two Figure 7 The taSSAW nano-particles separation device Figure 8 Mechanisms of acoustofluidic base system for isolating exosomes Figure 9 Conceptual view of the sheathless particle separator using SSAW pressure nodes at the side wall in the separation stage. Later, like (Ma et al, 2016b), another group introduce a single stage conducting liquid based SSAW sheathless microfluidic device to separate micro-particles and extended the study to get concentrated malaria parasites for rapid detection of the malarial infection (Nam et al, 2017).…”

Section: Sheathless Separation Techniquesmentioning

confidence: 99%

Evaluation of acoustic-based particle separation methods

Ahmad

Bozkurt

Farhanieh

2019

WJE

View full text Add to dashboard Cite

Purpose This paper aims to Separation and sorting of biological cells is desirable in many applications for analyzing cell properties, such as disease diagnostics, drugs delivery, chemical processing and therapeutics. Design/methodology/approach Acoustic energy-based bioparticle separation is a simple, viable, bio-compatible and contact-less technique using, which can separate the bioparticles based on their density and size, with-out labeling the sample particles. Findings Conventionally available bioparticle separation techniques as fluorescence and immunomagnetic may cause a serious threat to the life of the cells due to various compatibility issues. Moreover, they also require an extra pre-processing labeling step. Contrarily, label-free separation can be considered as an alternative solution to the traditional bio-particle separation methods, due to their simpler operating principles and lower cost constraints. Acoustic based particle separation methods have captured a lot of attention among the other reported label-free particle separation techniques because of the numerous advantages it offers. Practical implications This study tries to briefly cover the developments of different acoustic-based particle separation techniques over the years. Unlike the conventional surveys on general bioparticles separation, this study is focused particularly on the acoustic-based particle separation. The study would provide a comprehensive guide for the future researchers especially working in the field of the acoustics, in studying and designing the acoustic-based particle separation techniques. Originality/value The study insights a brief theory of different types of acoustic waves and their interaction with the bioparticles is considered, followed by acoustic-based particle separation devices reported till the date. The integration of acoustic-based separation techniques with other methods and with each other is also discussed. Finally, all major aspects like the approach, and productivity, etc., of the adopted acoustic particle separation methods are sketched in this article.

show abstract

Continuous sheathless microparticle and cell patterning using CL-SSAWs (conductive liquid-based standing surface acoustic waves)

Cited by 10 publications

References 47 publications

A discrete dielectrophoresis device for the separation of malaria‐infected cells

A discrete dielectrophoresis device for the separation of malaria‐infected cells

Microparticle self-assembly induced by travelling surface acoustic waves

Evaluation of acoustic-based particle separation methods

Contact Info

Product

Resources

About