Continuous simultaneous measurement of plasma protein concentration and hematocrit in the alert rat. A short preliminary report

A refractometric method was used for the continuous registration of plasma protein concentration in rats during and after 3 and 5 minutes of provoked activity. Simultaneous conductometric measurements of hematocrit (hct) showed that, although both invariably changing in the same direction, the relative change of protein concentration is always less than that of hct: plasma volume changes calculated from the former fall short of those calculated from hct by 34 +/- 23% during the hemoconcentrative period during activity and by 52 +/- 11% during the hemodilutory period after activity. The difference between these figures was significant, thus implying that fluid leaving the circulation during the filtration phase is less rich in protein than that entering it during the absorptive phase of microvascular adjustments. A kinetic analysis of the period after activity was made. The rate constants of fluid- and protein-flux were closely correlated. Both plasma volume and intravascular protein mass increased asymptotically to a new equilibrium 6% above control within 30 min after activity. It is suggested that the excess protein is mobilized from large parenchymatous organs, mainly the liver.

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Continuous measurement of plasma protein content in the alert rat during and after 3 to 5 minutes of moderate activity

Mösinger

Aziz

Lindt

1986

Basic Res Cardiol

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On-line Monitoring of the Intravascular Volume during Haemodialysis by Continuous Refractometry

Kuhlmann

Aziz

et al. 1999

Int J Artif Organs

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The control of intravascular volume (IVV) by continuous on-line measurement of protein concentration would optimise the patients' specific rate of ultrafiltration. To prove the accuracy of a refractometric device, plasma was continuously drawn by haemofiltration during 10 haemodialysis treatments of male patients. Refractometry reflects highly significant changes in the concentrations of filtrate proteins (r = 0.862, p < 0.001) and blood proteins (rtotal = 0.593, ptotal < 0.001). In vitro, the refractometric device detected a change of protein concentration of 0.041 g/L through a refraction increase of 0.1 mV. The power of discrimination was 0.067% of IVV However, in vivo, the accuracy of IVV refractometric monitoring is reduced by interference factors such as sodium (0. 141 mV/mmol/L), glucose (0.034 mV/mg/dl) and triglycerides (-0.040 mV/mg/dl). Adjustment of the refraction data using sodium and glucose electrodes and plasma filters with a cut-off below the size of chylomicrons is recommended.

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Continuous simultaneous measurement of plasma protein concentration and hematocrit in the alert rat. A short preliminary report

Cited by 2 publications

References 7 publications

Continuous measurement of plasma protein content in the alert rat during and after 3 to 5 minutes of moderate activity

Continuous measurement of plasma protein content in the alert rat during and after 3 to 5 minutes of moderate activity

On-line Monitoring of the Intravascular Volume during Haemodialysis by Continuous Refractometry

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