Continuous subcutaneous insulin infusion ameliorates bone structures and mechanical properties in type 2 diabetic rats by regulating bone remodeling

Shi, Peipei; Hou, Aiqi; Li, Chenchen; Wu, Xiaodan; Jia, Shaowei; Cen, Haipeng; Hu, Xiaorong; Gong, He

doi:10.1016/j.bone.2021.116101

Cited by 10 publications

(14 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As previously commented, unhealthy catch-up growth correlates to higher risk of metabolic diseases, such as diabetes and obesity, later in life [ 7 ]. Moreover, diabetic, and obese adult patients are at higher risk of bone fractures due to impaired bone microstructure [ 42 , 46 ]. Overall, preventing the development of a thrifty phenotype characterized by impaired insulin sensitivity might exert short- and long-term beneficial effects on bone health parameters (i.e., BMD and bone trabecular and cortical microstructure) of children undergoing catch-up growth, leading to a higher peak bone mass at the end of their adolescence.…”

Section: Discussionmentioning

confidence: 99%

Dietary Complex and Slow Digestive Carbohydrates Promote Bone Mass and Improve Bone Microarchitecture during Catch-Up Growth in Rats

Bueno-Vargas¹,

Manzano²,

Pérez-Castillo³

et al. 2022

Nutrients

View full text Add to dashboard Cite

Catch-up growth is a process that promotes weight and height gains to recover normal growth patterns after a transient period of growth inhibition. Accelerated infant growth is associated with reduced bone mass and quality characterized by poor bone mineral density (BMD), content (BMC), and impaired microarchitecture. The present study evaluated the effects of a diet containing slow (SDC) or rapid (RDC) digestible carbohydrates on bone quality parameters during the catch-up growth period in a model of diet-induced stunted rats. The food restriction period negatively impacted BMD, BMC, and microarchitecture of appendicular and axial bones. The SDC diet was shown to improve BMD and BMC of appendicular and axial bones after a four-week refeeding period in comparison with the RDC diet. In the same line, the micro-CT analysis revealed that the trabecular microarchitecture of tibiae and vertebrae was positively impacted by the dietary intervention with SDC compared to RDC. Furthermore, features of the cortical microstructure of vertebra bones were also improved in the SDC group animals. Similarly, animals allocated to the SDC diet displayed modest improvements in growth plate thickness, surface, and volume compared to the RDC group. Diets containing the described SDC blend might contribute to an adequate bone formation during catch-up growth thus increasing peak bone mass, which could be linked to reduced fracture risk later in life in individuals undergoing transient undernutrition during early life.

show abstract

Section: Discussionmentioning

confidence: 99%

Dietary Complex and Slow Digestive Carbohydrates Promote Bone Mass and Improve Bone Microarchitecture during Catch-Up Growth in Rats

Bueno-Vargas¹,

Manzano²,

Pérez-Castillo³

et al. 2022

Nutrients

View full text Add to dashboard Cite

show abstract

“…Then they were injected with fresh streptozotocin solution (STZ) at a dose of 35 mg/kg [ 33 , 34 ]. After 7 days, blood glucose of the caudal vein in the fasting state was measured, and the blood glucose concentration of the rats greater than 16.7 mmol/L was defined as type 2 diabetes mellitus rats [ 35 , 36 ]. A total of 55 rats were successfully modeled, which were randomly divided into 5 groups: 10 rats in T2DM group, 11 rats in VER 4 group, 11 rats in VER 12 group, 11 rats in VER 24 group, and 12 rats in VER 48 group.…”

Section: Methodsmentioning

confidence: 99%

“…The rat model of type 2 diabetes was induced by STZ in combination with high-fat and high-carbohydrate chow. After modeling, the phenotypes of the rat model of type 2 diabetes were insulin resistance and insufficient insulin secretion, increased blood glucose, polyuria, weight loss, fatigue and vision loss [ 35 , 37 , 38 ].…”

Section: Methodsmentioning

confidence: 99%

“…Wherein, L is the span of fulcrum, ΔF / Δx is the slope of the force-displacement curve, I is the moment of inertia of the cross-section at the fracture site of the femur sample, I = π ( D 4 − d 4 )/ 64 , and the cross-section shape of the middle segment of the femur is simplified as a circular ring [ 35 ], D and d are the outer and inner diameters of cortical bone at the fracture position, respectively.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effect of verapamil on bone mass, microstructure and mechanical properties in type 2 diabetes mellitus rats

Gong

et al. 2022

BMC Musculoskelet Disord

Self Cite

View full text Add to dashboard Cite

Background Verapamil was mainly used to treat hypertension, cardiovascular disease, inflammation and improve blood glucose in patients with diabetes, but its effects on bone mass, microstructure and mechanical properties were unclear. This study described the effects of verapamil on bone mass, microstructure, macro and nano mechanical properties in type 2 diabetic rats. Methods Rat models of type 2 diabetes were treated with verapamil at doses of 4, 12, 24 and 48 mg/kg/day by gavage respectively, twice a day. After 12 weeks, all rats were sacrificed under general anesthesia. Blood glucose, blood lipid, renal function and biochemical markers of bone metabolism were obtained by serum analysis, Micro-CT scanning was used to assess the microstructure parameters of cancellous bone of femoral head, three-point bending test was used to measure maximum load and elastic modulus of femoral shaft, and nano-indentation tests were used to measure indentation moduli and hardnesses of longitudinal cortical bone in femoral shaft, longitudinal and transverse cancellous bones in femoral head. Results Compared with T2DM group, transverse indentation moduli of cancellous bones in VER 24 group, longitudinal and transverse indentation moduli and hardnesses of cancellous bones in VER 48 group were significantly increased (p < 0.05). Furthermore, the effects of verapamil on blood glucoses, microstructures and mechanical properties in type 2 diabetic rats were dependent on drug dose. Starting from verapamil dose of 12 mg/kg/day, with dose increasing, the concentrations of P1NP, BMD, BV/TV, Tb. Th, Tb. N, maximum loads, elastic moduli, indentation moduli and hardnesses of femurs in rats in treatment group increased gradually, the concentrations of CTX-1 decreased gradually, but these parameters did not return to the level of the corresponding parameters of normal rats. Verapamil (48 mg/kg/day) had the best therapeutic effect. Conclusion Verapamil treatment (24, 48 mg/kg/day) significantly affected nano mechanical properties of the femurs, and tended to improve bone microstructures and macro mechanical properties of the femurs, which provided guidance for the selection of verapamil dose in the treatment of type 2 diabetic patients.

show abstract

“…In early T1D, CSII treatment maintained the normal structure and strength, while delayed CSII treatment only partially restored the structure and strength of femoral cortical and cancellous bones in mice [ 25 ]. For T2D, in a previous study of our group, 4-week and 8-week CSII treatments are found to improve the microstructure, mineral composition and macro-nano mechanical properties of the femur of T2D rats by affecting bone metabolism, bone formation and bone resorption, and 8-week CSII treatment is more effective than 4-week CSII treatment [ 26 ]. T2D patients have different fracture probabilities at different sites [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of continuous subcutaneous insulin infusion on the microstructures, mechanical properties and bone mineral compositions of lumbar spines in type 2 diabetic rats

Gong

Hou

et al. 2022

BMC Musculoskelet Disord

Self Cite

View full text Add to dashboard Cite

Background Continuous subcutaneous insulin infusion (CSII) for the treatment of type 2 diabetes (T2D) can improve the structure and strength of femur of rats, but the effect of CSII treatment on the lumbar spine of T2D rats is unknown. The purpose of this study is to investigate the effects of CSII on the microstructure, multi-scale mechanical properties and bone mineral composition of the lumbar spine in T2D rats. Methods Seventy 6-week-old male Sprague–Dawley (SD) rats were divided into two batches, each including Control, T2D, CSII and Placebo groups, and the duration of insulin treatment was 4-week and 8-week, respectively. At the end of the experiment, the rats were sacrificed to take their lumbar spine. Microstructure, bone mineral composition and nanoscopic-mesoscopic-apparentand-macroscopic mechanical properties were evaluated through micro-computed tomography (micro-CT), Raman spectroscopy, nanoindentation test, nonlinear finite element analysis and compression test. Results It was found that 4 weeks later, T2D significantly decreased trabecular thickness (Tb.Th), nanoscopic-apparent and partial mesoscopic mechanical parameters of lumbar spine (P < 0.05), and significantly increased bone mineral composition parameters of cortical bone (P < 0.05). It was shown that CSII significantly improved nanoscopic-apparent mechanical parameters (P < 0.05). In addition, 8 weeks later, T2D significantly decreased bone mineral density (BMD), bone volume fraction (BV/TV) and macroscopic mechanical parameters (P < 0.05), and significantly increased bone mineral composition parameters of cancellous bone (P < 0.05). CSII treatment significantly improved partial mesoscopic-macroscopic mechanical parameters and some cortical bone mineral composition parameters (P < 0.05). Conclusions CSII treatment can significantly improve the nanoscopic-mesoscopic-apparent-macroscopic mechanical properties of the lumbar spine in T2D rats, as well as the bone structure and bone mineral composition of the lumbar vertebrae, but it will take longer treatment time to restore the normal level. In addition, T2D and CSII treatment affected bone mineral composition of cortical bone earlier than cancellous bone of lumbar spine in rat. Our study can provide evidence for clinical prevention and treatment of T2D-related bone diseases.

show abstract

Continuous subcutaneous insulin infusion ameliorates bone structures and mechanical properties in type 2 diabetic rats by regulating bone remodeling

Cited by 10 publications

References 58 publications

Dietary Complex and Slow Digestive Carbohydrates Promote Bone Mass and Improve Bone Microarchitecture during Catch-Up Growth in Rats

Dietary Complex and Slow Digestive Carbohydrates Promote Bone Mass and Improve Bone Microarchitecture during Catch-Up Growth in Rats

Effect of verapamil on bone mass, microstructure and mechanical properties in type 2 diabetes mellitus rats

Effects of continuous subcutaneous insulin infusion on the microstructures, mechanical properties and bone mineral compositions of lumbar spines in type 2 diabetic rats

Contact Info

Product

Resources

About