Continuous volumetric imaging via an optical phase-locked ultrasound lens

Kong, Lingjie; Tang, Juliet D.; Little, Justin P.; Yu, Yongmei; Lämmermann, Tim; Lin, Charles P.; Germain, Ronald N.; Cui, Meng

doi:10.1038/nmeth.3476

Cited by 159 publications

(138 citation statements)

References 28 publications

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“…As we have demonstrated before [24][25][26]40], IMPACT works well in the ballistic regime for in vivo imaging, and the compensation wavefront can be effective for >60 minutes when imaging mouse cerebral cortex [17]. Further details of the two techniques can be found in our previous work [16,24].…”

Section: Experimental Setup and Principlesmentioning

confidence: 81%

“…The fast volumetric imaging also enabled us to capture rare events. In addition to microglia, some leukocytes circulating in the vasculature of the Cx3cr1 GFP/GFP mice also express GFP [16]. In Figs.…”

Section: Resultsmentioning

confidence: 96%

“…Novel development to achieve simultaneous large volume correction is needed. Additionally, the OPLUL induced dynamical aberrations (mainly spherical aberrations) [16], which requires fast dynamic compensation at ~450 kHz. We have proposed to cancel the dynamical aberrations with another UL [16], which however is quite complex.…”

Section: Discussionmentioning

confidence: 99%

“…They were for generating oscillating defocusing wavefront thus for fast axial scanning [16,35] and compensating wavefront distortions [24,36], respectively. In practice, the resonance of the UL may drift resulting from laser heating or fluctuations of environment temperature.…”

Section: Experimental Setup and Principlesmentioning

confidence: 99%

“…Meanwhile, the optimized trajectory based scanning methods, such as random access microscopy, are susceptible to motion artifacts [15]. Recently, we have reported a high-speed volumetric imaging system based on optical phase-locked ultrasound lens (OPLUL), where the microsecond scale axial scanning is achieved by conjugating the oscillating defocusing wavefront generated by OPLUL to the pupil plane of the objective lens [16]. We have applied this system in calcium imaging of neuron network activity in the cerebral cortex of behaving mice, and transient morphology imaging of immune cells.…”

Section: Introductionmentioning

confidence: 99%

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In vivo volumetric imaging of biological dynamics in deep tissue via wavefront engineering

Kong¹,

Tang²,

Cui³

2016

Opt. Express

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Biological systems undergo dynamical changes continuously which span multiple spatial and temporal scales. To study these complex biological dynamics in vivo, high-speed volumetric imaging that can work at large imaging depth is highly desired. However, deep tissue imaging suffers from wavefront distortion, resulting in reduced Strehl ratio and image quality. Here we combine the two wavefront engineering methods developed in our lab, namely the optical phase-locked ultrasound lens based volumetric imaging and the iterative multiphoton adaptive compensation technique, and demonstrate in vivo volumetric imaging of microglial and mitochondrial dynamics at large depth in mouse brain cortex and lymph node, respectively.

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Section: Experimental Setup and Principlesmentioning

confidence: 81%

Section: Resultsmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Experimental Setup and Principlesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In vivo volumetric imaging of biological dynamics in deep tissue via wavefront engineering

Kong¹,

Tang²,

Cui³

2016

Opt. Express

Self Cite

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Understanding the In Vivo Fate of Advanced Materials by Imaging

Garlin

et al. 2020

Adv Funct Materials

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Engineered materials are ubiquitous in biomedical applications ranging from systemic drug delivery systems to orthopedic implants, and their actions unfold across multiple time‐ and length‐scales. The efficacy and safety of biologics, nanomaterials, and macroscopic implants are all dictated by the same general principles of pharmacology as applied to small molecule drugs, comprising how the body affects materials (pharmacokinetics, PK) and conversely how materials affect the body (pharmacodynamics, PD). Imaging technologies play an increasingly insightful role in monitoring both of these processes, often simultaneously: translational macroscopic imaging modalities such as magnetic resonance imaging and positron emission tomography/computed tomography offer whole‐body quantitation of biodistribution and structural or molecular response, while ex vivo approaches and optical imaging via in vivo (intravital) microscopy reveal behaviors at subcellular resolution. In this review, the authors survey developments in imaging the in situ behavior of systemically and locally administered materials, with a particular focus on using microscopy to understand transport, target engagement, and downstream host responses at a single‐cell level. The themes of microenvironmental influence, controlled drug release, on‐target molecular action, and immune response, especially as mediated by macrophages and other myeloid cells, are examined. Finally, the future directions of how new imaging technologies may propel efficient clinical translation of next‐generation therapeutics and medical devices are proposed.

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Dynamic Multifocus Laser Writing with Acousto‐Optofluidics

Zunino

Surdo

2019

Adv Materials Technologies

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Laser writing of materials is normally performed by the sequential scanning of a single focused beam across a sample. This process is time‐consuming and it can severely limit the throughput of laser systems in key applications such as surgery, microelectronics, or manufacturing. A parallelization strategy based on ultrasound waves in a liquid to diffract light into multiple beamlets is reported. Adjusting amplitude, frequency, or phase of ultrasound allows tunable multifocus distributions with sub‐microsecond control. When combined with sample translation, the dynamic splitting of light leads to high‐throughput laser processing, as demonstrated by locally modifying the morphological and wettability properties of metals, polymers, and ceramics. The results illustrate how acousto‐optofluidic systems are universal tools for fast multifocus generation, with potential impact in fields such as imaging or optical trapping.

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Continuous volumetric imaging via an optical phase-locked ultrasound lens

Cited by 159 publications

References 28 publications

In vivo volumetric imaging of biological dynamics in deep tissue via wavefront engineering

In vivo volumetric imaging of biological dynamics in deep tissue via wavefront engineering

Understanding the In Vivo Fate of Advanced Materials by Imaging

Dynamic Multifocus Laser Writing with Acousto‐Optofluidics

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