Contraceptive gossypol blocks cell-to-cell communication in human and rat cells

Hervé, Jean-Claude; Pluciennik, F.; Bastide, Bruno; Cronier, Laurent; Verrecchia, Franck; Malassiné, André; Joffre, M.; Délèze, J.

doi:10.1016/0014-2999(96)00476-1

Cited by 34 publications

(19 citation statements)

References 44 publications

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“…In one study, evidence indicated that gossypol may alter amplitude-and frequency-encoded information derived from cell signaling events which could result in altered homeostasis [78]. Also, 1 irreversibly impaired the intercellular communication between homologous pairs of various cultured cells from man or rat [79]. In a similar investigation, 1 inhibited gap junctional intercellular communication in some cell lines, e.g., the Leydig cell, which is the component of a target organ system for toxicity [80].…”

Section: Cell Communicationmentioning

confidence: 93%

Mechanism of Drug and Toxic Actions of Gossypol: Focus on Reactive Oxygen Species and Electron Transfer

Kovacic¹

2003

CMC

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Gossypol, a constituent of cottonseeds, displays various drug properties, including antifertility and anticancer. Toxicity is shown against the reproductive system, heart, liver, and membranes. The compound exhibits pro- and anti-oxidant behavior. Electron transfer (ET) functionalities, present in gossypol and its metabolites, comprise conjugated dicarbonyl, a quinone derivative, Shiff bases, and metal complexes. The parent possesses a reduction potential favorable for in vivo ET. Considerable evidence points to oxidative stress (OS), formation of reactive oxygen species (ROS), and DNA scission, characteristics of redox cycling by ET in biosystems. Mechanistic aspects are addressed with OS-ROS-ET as the guiding theme, in addition to other modes of action resulting in a multifaceted scenario.

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Section: Cell Communicationmentioning

confidence: 93%

Mechanism of Drug and Toxic Actions of Gossypol: Focus on Reactive Oxygen Species and Electron Transfer

Kovacic¹

2003

CMC

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“…1-oleoyl-2-acetyl-sn-glycerol, testosterone, 17β-estradiol, gossypol, H7) were similarly applied. Gossypol (5 µM), for example, elicited a complete electrical uncoupling between ventricular myocytes within only 4 min whereas a small but measurable dye diffusion still persisted after 15 min of exposure [22]. Whole-cell conditions might render the cells much more fragile than the non-invasive gapFRAP method.…”

Section: Resultsmentioning

confidence: 98%

The metabolic inhibitor antimycin A can disrupt cell-to-cell communication by an ATP- and Ca 2+ -independent mechanism

Plaisance

Duthe

Sarrouilhe

et al. 2003

Pfl�gers Archiv European Journal of Physiology

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In cardiac myocytes of new-born rats, the degree of intercellular communication through gap junctional channels closely depends on the metabolic state of the cells. In contrast, in stably transfected HeLa cells expressing rat cardiac connexin43 (Cx43, the main channel-forming protein present in ventricular myocytes), a major part of junctional communication persisted in ATP-depleted conditions, in the presence of a metabolic inhibitor (KCN) or of a broad spectrum inhibitor of protein kinases (H7). However, another metabolic inhibitor, antimycin A, which like cyanide inhibits electron transfer in the respiratory chain, totally interrupted cell-to-cell communication between Cx43-HeLa cells, even in whole-cell conditions, when ATP (5 mM) was present. Antimycin A caused a modest increase in cytosolic calcium concentration; however, junctional uncoupling still occurred when this rise was prevented. Conditions of ischemic insult (e.g. ischemia or chemical hypoxia) frequently cause the activation of protein kinases, particularly of Src and MAP kinases, and such activations are known to markedly disrupt gap junctional communication. Antimycin-induced junctional uncoupling occurred even in the presence of inhibitors of these kinases. Antimycin A appears able to cause junctional uncoupling either through the ATP depletion it induces as a metabolic poison or via a direct action on gap junction constituents.

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“…Pemberian ekstrak biji kapas berkaitan dengan lingkungan folikel yang dapat memengaruhi fertilitas betina (Sirard et al, 2006). Beberapa studi menyatakan kerusakan akibat gosipol juga disebabkan oleh hambatan sekresi adrenocorticotropic hormone (ACTH), hambatan pembentukan cyclic adenosine monophosphate (cAMP) pada sel-sel luteal (Lin et al, 1994b), gangguan komunikasi interseluler (Hervé et al, 1996), gangguan metabolisme energi seluler, gangguan transpor ion melalui membran, penurunan steroidogenesis yaitu estrogen dan progesteron (Gadelha et al, 2014b).…”

Section: Hasil Dan Pembahasanunclassified

Pengendalian Folikulogenesis Ovarium dengan Pemberian Ekstrak Biji Kapas

Ramadhani

Supriatna

Karja

et al. 2017

Jurnal Sain Veteriner

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Gosipol is a substances contained in extracted cotton seed which is thought to have the antifertility ability therefore it is often used as a herbal contraceptive. The aim of this study were to assess the folliculogenesis in mice after administrated with cottonseed extract. 60 female mice strain DDY which was 14-15 weeks old and 30-35 g body weight were divided into five groups and given cottonseed extract each 0; 1,5; 2,1 and 2,7 g/kg BW for 5, 10, 15, 24, and 24 + 10 days (without cottonseed treatment). At the end of the treatment period, mice was euthanasia to observe follicular development histomorphology (each three mice of each treatment). Mice estrous status were evaluated based on the description of the vaginal smear cells with Giemsa staining. The results showed that the number of developing follicles was low (P < 0.05) compared with control after 5 days cottonseed extract administration at dose 2,7 g/kg BW that were 23 ± 3,6. At dose 1,5 and 2,1 g/kg BW the number of follicles was low after 24 days that were 25 ± 10,4 and 27 ± 3,5. Recovery effects of follicle number after cottonseed extract administration for 24 days was the best at a dose of 1,5 g/kg BW. Prolonge of estrous cycle occured in mice which were administrated the cottonseed extract of at all dose treatment. In conclusion, although the decrease in the number of developing follicles and prolonge of estrous cycles occurred after cottonseed extract administration, but these effects are reversible after the administration ended.Keywords: antifertility, cotton seed, folliculogenesis, gossypol AbstrakGosipol adalah zat yang terkandung dalam ekstrak biji kapas dan diduga mempunyai kemampuan antifertilitas sehingga sering digunakan sebagai bahan herbal kontrasepsi. Penelitian ini bertujuan mengkaji gambaran folikulogenesis pada mencit yang diberi ekstrak biji kapas. Sebanyak 60 ekor induk mencit betina strain DDY berumur 14-15 minggu dengan bobot badan 30-35 g dibagi menjadi 4 kelompok dan diberi ekstrak biji kapas masing-masing 0; 1,5; 2,1 dan 2,7 g/kg BB selama 5, 10, 15, 24 dan 24 + 10 hari (tanpa pemberian ekstrak biji kapas). Pada setiap akhir waktu pengamatan, mencit di-euthanasia untuk mengamati histomorfologi perkembangan folikel (masing-masing tiga ekor dari tiap perlakuan). Status estrus mencit dievaluasi berdasarkan gambaran sel dari ulas vagina dengan pewarnaan Giemsa. Hasil penelitian menunjukkan bahwa jumlah folikel rendah (P < 0,05) dibandingkan dengan kontrol setelah 5 hari pemberian ekstrak biji kapas dengan dosis 2,7 g/kg BB yaitu 23 ± 3,6. Pada dosis 1,5 dan 2,1 g/kg BB jumlah folikel menjadi rendah setelah 24 hari yaitu 25 ± 10,4; 27 ± 3,5. Efek pemulihan jumlah folikel setelah pemberian ekstrak biji kapas selama 24 hari paling baik pada dosis 1,5 g/kg BB. Pemanjangan siklus estrus terjadi pada mencit yang diberi ekstrak bji kapas pada semua dosis perlakuan. Berdasarkan hasil tersebut dapat disimpulkan bahwa walaupun terjadi penurunan jumlah folikel yang berkembang dan terjadi pemanjangan siklus estrus setelah pemberian ...

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Contraceptive gossypol blocks cell-to-cell communication in human and rat cells

Cited by 34 publications

References 44 publications

Mechanism of Drug and Toxic Actions of Gossypol: Focus on Reactive Oxygen Species and Electron Transfer

Mechanism of Drug and Toxic Actions of Gossypol: Focus on Reactive Oxygen Species and Electron Transfer

The metabolic inhibitor antimycin A can disrupt cell-to-cell communication by an ATP- and Ca 2+ -independent mechanism

Pengendalian Folikulogenesis Ovarium dengan Pemberian Ekstrak Biji Kapas

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