Contractile activation and measurements of intracellular Ca2+ concentration in cane toad twitch fibres in the presence of 2,3‐butanedione monoxime

Lyster, D. J. K.; Stephenson, D. George

doi:10.1113/expphysiol.1995.sp003866

Cited by 8 publications

(5 citation statements)

References 22 publications

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“…Additional evidence is provided by the results reported here showing the independence of the static stiffness from BDM concentration up to 10 mM. BDM has been shown to have several effects on muscle contraction, the most important of which are a direct inhibition of actomyosin interaction and a reduction of calcium released upon stimulation (Fryer et al, 1988;Horiuti et al, 1988;Higuchi and Takemori, 1989;Lyster and Stephenson, 1995;Maylie and Hui, 1991;Tripathy et al, 1999). However, these effects are strongly species-dependent.…”

Section: Discussionsupporting

confidence: 60%

“…After a test of fiber viability and a measure of the isometric tetanic tension (P 0 ) in normal Ringer's solution, all the experiments were made in Ringer's with 2,3-butanedione monoxime (BDM) added at concentration between 6 and 10 mM. The use of BDM was necessary to inhibit crossbridge formation (Horiuti et al, 1988;Higuchi and Takemori, 1989;Lyster and Stephenson, 1995) because the relatively large and fast stretches necessary to measure the static stiffness quickly damaged fibers developing normal tetanic force (in normal Ringer's solution). In addition, crossbridge inhibition also reduced cross-bridge contribution to the force transient evoked by the stretch, thus isolating components arising from other mechanical structures of the sarcomere.…”

Section: Methodsmentioning

confidence: 99%

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A Non-Cross-Bridge Stiffness in Activated Frog Muscle Fibers

Bagni

Cecchi

Colombini

et al. 2002

Biophysical Journal

108

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Force responses to fast ramp stretches of various amplitude and velocity, applied during tetanic contractions, were measured in single intact fibers from frog tibialis anterior muscle. Experiments were performed at 14 degrees C at approximately 2.1 microm sarcomere length on fibers bathed in Ringer's solution containing various concentrations of 2,3-butanedione monoxime (BDM) to greatly reduce the isometric tension. The fast tension transient produced by the stretch was followed by a period, lasting until relaxation, during which the tension remained constant to a value that greatly exceeded the isometric tension. The excess of tension was termed "static tension," and the ratio between the force and the accompanying sarcomere length change was termed "static stiffness." The static stiffness was independent of the active tension developed by the fiber, and independent of stretch amplitude and stretching velocity in the whole range tested; it increased with sarcomere length in the range 2.1-2.8 microm, to decrease again at longer lengths. Static stiffness increased well ahead of tension during the tetanus rise, and fell ahead of tension during relaxation. These results suggest that activation increased the stiffness of some sarcomeric structure(s) outside the cross-bridges.

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Section: Discussionsupporting

confidence: 60%

Section: Methodsmentioning

confidence: 99%

A Non-Cross-Bridge Stiffness in Activated Frog Muscle Fibers

Bagni

Cecchi

Colombini

et al. 2002

Biophysical Journal

108

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“…BDM is believed to depress, reversibly, actively generated tension both by reducing calcium release and by suppressing crossbridge cycling. The mode of action appears to be different in different muscles and in those of different species (Lyster & Stephenson, 1995). BDM does not appear to influence the tension response of relaxed rat skeletal muscle to stretch (Mutungi & Ranatunga, 1996b).…”

Section: Discussionmentioning

confidence: 85%

A cross‐bridge mechanism can explain the thixotropic short‐range elastic component of relaxed frog skeletal muscle

Campbell

Lakie

1998

The Journal of Physiology

122

130

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The precise control of posture and movement imposes severe constraints on the human neuromuscular system. In particular, to maintain stability, the motor control system must continually react to proprioceptive feedback as the body is perturbed by unpredictable external forces. In this type of control mechanism there is an inevitable time delay (due to e.g. nerve conduction and electromechanical coupling) between the sensing of the perturbation and the completion of the corrective movement. The delays in the feedback loop are destabilizing and may result in tremulous movement (McMahon, 1984). A small resistance to movement inherent in the muscle fibres themselves would provide Journal of Physiology (1998) 1. The passive tension and sarcomere length of relaxed frog skeletal muscle fibres were measured in response to imposed length stretches. The tension response to a constantvelocity stretch exhibited a clear discontinuity. Tension rose more rapidly during the initial • 0·4 % LÑ of the stretch than during the latter stages (where LÑ is the resting length of the fibre). This initial tension response is attributed to the short-range elastic component (SREC). 2. The use of paired triangular stretches revealed that the maximum tension produced during the SREC response of the second stretch was significantly reduced by the first stretch. This history-dependent behaviour of the SREC reflects thixotropic stiffness changes that have been previously described in relaxed muscle. 3. The biphasic nature of the SREC tension response to movement was most apparent during the first imposed length change after a period at a fixed length, irrespective of the direction of movement. 4. If a relaxed muscle was subjected to an imposed triangular length change so that the muscle was initially stretched and subsequently shortened back to its original fibre length, the resting tension at the end of the stretch was reduced relative to its initial pre-stretch value. Following the end of the stretch, tension slowly increased towards its initial value but the tension recovery was not accompanied by a contemporaneous increase in sarcomere length. This finding suggests that the resting tension of a relaxed muscle fibre is not entirely due to passive elasticity. The results are compatible with the suggestion that a portion of the resting tension -the filamentary resting tension (FRT) -is produced by a low level of active force generation. 5. If a second identical stretch was imposed on the muscle at a time when the resting tension was reduced by the previous stretch, the maximal tension produced during the second stretch was the same as that produced during the first, despite the second stretch commencing from a lower initial resting tension. 6. In experiments using paired triangular length changes, an inter-stretch interval of zero did not produce a substantially greater thixotropic reduction in the second stretch elastic limit force than an inter-stretch interval in the range 0·5-1 s. 7. A theoretical model was developed in which the SREC and ...

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“…1994). A recent study by Lyster & Stephenson (1995) has shown that the effects of BDM on the cane toad muscle appear to be intermediate between those of mammalian and frog muscles.…”

mentioning

confidence: 98%

“…1988, Horiuti et al . 1988, Maylie & Hui 1991, Lyster & Stephenson 1995). There is also evidence that these actions of BDM may vary from one animal species to another.…”

mentioning

confidence: 99%

2,3‐Butanedione monoxime increases speed of relaxation in single muscle fibres of frog

Sun¹,

Lou²,

Edman³

2001

Acta Physiologica Scandinavica

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The effects of 2,3-butanedione monoxime (BDM) on intracellular Ca2+ transient and cross-bridge function were studied in frog single fibres from the anterior tibialis muscle of Rana temporaria (sarcomere length, 2.2 microm; temperature, 2-4 degrees C). The fluorescent dye fluo-3 was used to monitor the intracellular free calcium concentration ([Ca2+]i) during isometric contractions. BDM (1-5 mM) reduced the amplitude of the Ca2+ transient during twitches, but this effect was too small to explain the marked inhibition of BDM on twitch force. [Ca2+]i reached at the end of 1-s tetanic stimulation was not significantly affected by BDM (1.0 and 1.8 mM) while the maximum tetanic tension was substantially reduced. The rate of relaxation during isometric tetanus was increased by BDM whereas the rate of decay of the Ca2+ transient was reduced in the presence of BDM. The results strongly suggest that BDM, under the experimental conditions used, mainly affects the contractile machinery resulting in altered performance of the cross-bridges. These effects of BDM were evaluated in terms of the cross-bridge model of Huxley (1957) which was fitted to the experimental force-velocity data in the presence and absence of BDM

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Contractile activation and measurements of intracellular Ca2+ concentration in cane toad twitch fibres in the presence of 2,3‐butanedione monoxime

Cited by 8 publications

References 22 publications

A Non-Cross-Bridge Stiffness in Activated Frog Muscle Fibers

A Non-Cross-Bridge Stiffness in Activated Frog Muscle Fibers

A cross‐bridge mechanism can explain the thixotropic short‐range elastic component of relaxed frog skeletal muscle

2,3‐Butanedione monoxime increases speed of relaxation in single muscle fibres of frog

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