Contractile effects of endothelins on isolated ampullar segment of human oviduct in luteal phase of menstrual cycle

Janković, Slobodan M.; Janković, Snežana V.; Lukic, Goran; Radonjić, Vesela; Cupara, Snežana; Stefanović, Srđjan

doi:10.1016/j.phrs.2008.10.001

Cited by 6 publications

(7 citation statements)

References 17 publications

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“…by increasing endothelin production, a known inducer of myometrial contractility. (36-39) To confirm that oxidative stress is a labor associated change, we compared AF F2-IsoP from subjects not in labor undergoing cesarean sections to laboring women at term having normal vaginal deliveries. The latter had significantly higher AF F2-IsoP concentrations (0.52 ± 0.37 ng/ml) than the former subjects (0.36 ± 0.30; p = 0.004).…”

Section: Discussionmentioning

confidence: 99%

Amniotic Fluid Eicosanoids in Preterm and Term Births: Effects of Risk Factors for Spontaneous Preterm Labor

Menon

Fortunato

Milne

et al. 2011

Obstetrics &Amp; Gynecology

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OBJECTIVE To evaluate amniotic fluid (AF) arachidonic acid metabolites using enzymatic and nonenzymatic (lipid peroxidation) pathways in spontaneous preterm birth and term births, and to estimate whether prostanoid concentrations correlate with risk factors (race, cigarette smoking, and microbial invasion of amniotic cavity) associated with preterm birth. METHODS In a case-control study, AF was collected at the time of labor or during cesarean delivery. AF samples were subjected to gas chromatography, negative ion chemical ionization, and mass spectrometry for prostaglandin (PG)E2, PGF2α, and PGD2, 6-keto-PGF1α (6-KPGF1α, thromboxane (TXB2), and F2-isoprostane (F2-IsoP). Primary analysis examined differences between prostanoid concentrations in preterm birth (n=133) compared with term births (n=189). Secondary stratified analyses (by race, cigarette smoking and microbial invasion of amniotic cavity) compared eicosanoid concentrations in three epidemiological risk factors. RESULTS AF F2-IsoP, PGE2, and PGD2 were significantly higher at term than in PTB, whereas PGF2 α was higher in PTB 6-KPGF1α and TXB2 concentrations were not different. Data stratified by race (African American or Caucasian) showed no significant disparity among prostanoid concentrations. Regardless of gestational age status, F2-IsoP was threefold higher in smokers, and other eicosanoids were also higher in smokers compared to non-smokers. Preterm birth with microbial invasion of amniotic cavity had significantly higher F2-IsoP compared to preterm birth without microbial invasion of amniotic cavity. CONCLUSIONS Most AF eicosanoid concentrations (F2-isoP PGE2 and PGD2), are higher at term than in preterm birth. The only AF eicosanoid that is not higher at term is PGF2α.

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Section: Discussionmentioning

confidence: 99%

Amniotic Fluid Eicosanoids in Preterm and Term Births: Effects of Risk Factors for Spontaneous Preterm Labor

Menon

Fortunato

Milne

et al. 2011

Obstetrics &Amp; Gynecology

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“…It is unclear whether these responses are due to differences in affinity compared with ET‐1 or other factors such as the stability of peptides in this assay. Both ET‐1 and ET‐2 induced concentration‐dependent tonic contraction of the human isolated oviduct by acting on ET A receptors with similar EC 50 values (Jankovic et al ., 2009). However, ET‐1 also caused a concentration‐dependent inhibition of rhythmic contractions of the oviduct, with a similar potency whereas surprisingly ET‐2 had no effect.…”

Section: Unexplained Et‐2 Pharmacologymentioning

confidence: 99%

Endothelin‐2, the forgotten isoform: emerging role in the cardiovascular system, ovarian development, immunology and cancer

Ling

Maguire

Davenport

2012

British J Pharmacology

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Endothelin-2 [ET-2; also known as vasoactive intestinal contractor (VIC), in rodents] differs from endothelin-1 (ET-1) by only two amino acids, and unlike the third isoform, endothelin-3 (ET-3), it has the same affinity as ET-1 for both ETA and ETB receptors. It is often assumed that ET-2 would mimic the actions of the more abundant ET-1 and current pharmacological interventions used to inhibit the ET system would also block the actions of ET-2. These assumptions have focused research on ET-1 with ET-2 studied in much less detail. Recent research suggests that our understanding of the ET family requires re-evaluation. Although ET-2 is very similar in structure as well as pharmacology to ET-1, and may co-exist in the same tissue compartments, there is converging evidence for an important and distinct ET-2 pathway. Specifically is has been demonstrated that ET-2 has a key role in ovarian physiology, with ET-2-mediated contraction proposed as a final signal facilitating ovulation. Furthermore, ET-2 may also have a pathophysiological role in heart failure, immunology and cancer. Comparison of ET-2 versus ET-1 mRNA expression suggests this may be accomplished at the level of gene expression but differences may also exist in peptide synthesis by enzymes such as endothelin converting enzymes (ECEs) and chymase, which may allow the two pathways to be distinguished pharmacologically and become separate drug targets. LINKED ARTICLESThis article is part of a themed section on Endothelin. To view the other articles in this section visit http://dx.doi. org/ 10.1111/bph.2013.168.issue-2 Abbreviations ECE-1, endothelin converting enzyme 1; ECE-2, endothelin converting enzyme 2; ELISA, enzyme-linked immunosorbent assay; HIFs, hypoxia-inducible factors; MEK1/2, mitogen-activated protein kinase kinase; MMP-2, matrix metallopeptidase 2; MMP-9, matrix metallopeptidase 9; NEP, neutral endopeptidase; PRKO, progesterone receptor knockout; PMA, phorbol 12-myristate 13 acetate; VIC, vasoactive intestinal contractor IntroductionThe existence of a peptidic endothelium-derived constricting factor was originally proposed in 1985 by Hickey et al. (Hickey et al., 1985) but was identified and named endothelin (ET) by colleagues in 1988 (Yanagisawa et al., 1988). The peptide was renamed ET-1 to reflect the subsequent discovery a year later of human genes encoding two further members of the family, ET-2 and ET-3 (Inoue et al., 1989). The ET peptides act by activating two G-protein couples receptors, ETA and ETB. (Davenport, 2002;Alexander et al., 2011) ET-3 is the only endogenous isoform that distinguishes between the two receptors, having the same affinity at ETB as ET-1 and ET-2 but, at physiological concentrations, has much lower or little affinity for ETA. The established dogma is that ET-2 has the same affinity for both ETA and ETB receptors as ET-1. It is often assumed that ET-2 would mimic the actions of the more abundant peptide, and current pharmacological interventions used to inhibit the ET system would also block the actions o...

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“…EDNs produced by the endothelial cells are known to affect the vascular smooth muscle cells via EDNRs resulting in vasoconstriction (Kawanabe and Nauli 2011). In addition to its vasoactive effect, EDN1 stimulates the contraction of the bovine oviduct (Priyadarsana et al 2004) and EDN2 also induces oviductal contraction in human and rat (Al-Alem et al 2007;Jankovic et al 2009). EDNRA activation induces the contraction of smooth muscle in several tissues (Al-Alem et al 2007;Kawanabe and Nauli 2011), while EDNRB activation induces vasodilation by increasing endothelial NO production (Tsukahara et al 1994;Tykocki et al 2009).…”

Section: Discussionmentioning

confidence: 99%

“…2007) have been reported to affect oviductal contraction and relaxation. EDN also induces the contraction of oviductal smooth muscle in several species (Priyadarsana et al 2004;Al-Alem et al 2007;Jankovic et al 2009). EDN1 contracts the oviductal smooth muscle in cow (Wijayagunawardane et al 2001), and EDN2 induces the contraction of oviduct via EDNRA in rats (Al-Alem et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Endothelin as a local regulating factor in the bovine oviduct

Yamamoto

Kohka

Kobayashi

et al. 2016

Reprod. Fertil. Dev.

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Endothelin (EDN) is a possible regulating factor of oviductal motility, which is important for the transport of gametes and embryo. To clarify the factors that control the secretion of EDN in the bovine oviduct, the expression of EDNs, EDN-converting enzymes (ECEs) and EDN receptors (EDNRs) were investigated. All isoforms of EDN (EDN1-3), ECE (ECE1 and ECE2) and EDNR (EDNRA and EDNRB) were immunolocalised in the epithelial cells of the ampulla and the isthmus. EDNRs were also immunolocalised in smooth-muscle cells. The mRNA expression of EDN2 and ECE2 was higher in cultured ampullary oviductal epithelial cells than in isthmic cells. The expression of EDN1, EDN2 and ECE2 in the ampullary tissue was highest on the day of ovulation. Oestradiol-17β increased EDN2 and ECE1 expression, while progesterone increased only ECE1 expression in cultured ampullary epithelial cells. These results indicate that EDNs are produced by epithelial cells and their target site is smooth-muscle and epithelial cells, and suggest that ovarian steroids are regulators of endothelin synthesis in ampullary oviductal epithelial cells.

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Contractile effects of endothelins on isolated ampullar segment of human oviduct in luteal phase of menstrual cycle

Cited by 6 publications

References 17 publications

Amniotic Fluid Eicosanoids in Preterm and Term Births: Effects of Risk Factors for Spontaneous Preterm Labor

Amniotic Fluid Eicosanoids in Preterm and Term Births: Effects of Risk Factors for Spontaneous Preterm Labor

Endothelin‐2, the forgotten isoform: emerging role in the cardiovascular system, ovarian development, immunology and cancer

Endothelin as a local regulating factor in the bovine oviduct

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