Contractile Function in Nerve Allografted Primates

Fish, J.; Bain, Jerald; McKee, Nancy H.; Mackinnon, Susan E.; Plyley, M. J.

doi:10.1249/00005768-198904001-00398

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(2 citation statements)

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“…Only host immunosuppression with cyclosporin A was quantitatively assessed and demonstrated to prevent rejection and result in regeneration equivalent to autografts in rat [12][13][14][15][16][17][18] and primate models. [19][20][21][22] Pretreatment by freeze-thawing was similarly found to prevent rejection and allow similar regeneration to autografts as assessed by qualitative histology. 27,[29][30][31][32][33] In a rabbit model, Sanders and Young 25 and Gutmann and Sanders 23,24 stored nerves for 7, 14, or 21 days in Ringer's solution at 2°C prior to grafting.…”

Section: Discussionmentioning

confidence: 91%

“…Regeneration across nerve allografts in recipients immunosuppressed with cyclosporin A was equivalent to autografts in both rat [12][13][14][15][16][17][18] and primate models. [19][20][21][22] In the future, a necessary adjunct to clinical nerve allografting is the ability to store harvested nerves during transport. Furthermore, prolonged storage would increase access and allow time for testing of donor tissue and facilitate elective planning and less costly reconstructive procedures.…”

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confidence: 99%

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Regeneration across cold preserved peripheral nerve allografts

et al. 1999

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The feasibility of peripheral nerve allograft pretreatment utilizing cold storage (5 degrees C in the University of Wisconsin Cold Storage Solution) or freeze-thawing to prevent rejection was investigated. Regeneration across cold-stored (3 or 5 weeks) or freeze-thawed (FT), 3.0-cm sciatic nerve allografts were compared to fresh auto- and allografts in an inbred rat model. At 16-week post-engraftment, only FT allografts appeared similar to autografts on gross inspection; FT grafts were neither shrunken nor adherent to the surrounding tissue as seen in the other allograft groups. Qualitatively, the pattern of regeneration in the graft segments of the fresh allograft and to a lesser extent of pretreated allografts was inferior to that of autografts as evidenced by a disruption in the perineurium, more extrafascicular axons, smaller and fewer myelinated axons, increased intrafascicular collagen deposition, and the persistence of perineurial cell compartmentation and perivascular infiltrates. Distal to these grafts, the regeneration became more homogenous between groups, although areas of ongoing Wallerian degeneration, new regeneration as well as compartmentation, were more prevalent in fresh and pretreated allografts. Although the number of myelinated fibres was equivalent to autografts, the fibre diameters, the number of large diameter fibres, and the G-ratio were significantly decreased in the allograft groups, which, in part, accounted for the significant decrease in conduction velocity in the 3-week stored and fresh allograft, and the slight decrease in the 5-week stored and FT allograft groups. There was a small return in the Sciatic Function Index towards normal, but no consistent differences between groups were found. Prolonged cold storage and freeze-thawing of nerve allografts resulted in regeneration that was better than fresh allografts, but inferior to autografts. With the concomitant use of host immunosuppression or other immunotherapies, these storage techniques can provide a means of transporting nerve allografts between medical centres and for converting urgent into elective procedures.

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