Contractile reactivity of human myometrium in isolated non-pregnant uteri

Abstract

Continuous E2 treatment, with the addition of OT for the last 3 h of the 27 h perfusion period, led to the most pronounced uterotonic effects in the presented experimental condition.

Perfused Non-Pregnant Swine Uteri: A Model for Evaluating Transport Mechanisms to the Side Bearing the Dominant Follicle in Humans

“…The results presented here are in accordance with those first described by Bulletti et al [21] and those reported by Richter et al [23] in extracorporeal perfused human uteri. Both groups of authors described a characteristic increase in IUP after perfusion with estrogens.…”

“…Stimulation with highdose of 17β-estradiol increased myometrial OTR density, with maximum levels found in the uterine fundus [11]. In particular, pretreatment with 17β-estradiol before oxytocin stimulation resulted in a larger and more prolonged uterine response to oxytocin application [23], while progesterone stimulation directly resulted in a reduction of both the frequency and duration of uterine contractility, as reported by Bulletti et al [21].…”

“…Previous investigations validated the feasibility of this perfusion model of isolated uteri for various purposes [11,12,[18][19][20][21][22][23]. According to previously reported in vitro experiments, there is a possible clinical relationship between uterine contractility and steroid hormones [24,25].…”

“…Richter et al [11] reported that OTR expression is up-regulated by 17β-estradiol and oxytocin in nonpregnant uteri. Furthermore, the receptorm e d i a t e d r e a c t i v i t y o f t h e n o n -p r e g n a n t myometrium is modulated in a characteristic way by treatment with 17β-estradiol, oxytocin, or a combination of the two [23]. Stimulation with highdose of 17β-estradiol increased myometrial OTR density, with maximum levels found in the uterine fundus [11].…”

Differences in muscarinic-receptor agonist–, oxytocin-, and prostaglandin-induced uterine contractions

“…Previous investigations have validated the feasibility of this perfusion model of isolated uteri for various purposes (25)(26)(27)(28)(29)(30)(31). This perfusion model is able to keep the uterus in a functional condition and is suitable for the study of physiologic questions (32)(33)(34).…”