Contrapuntal role of ABA: Does it mediate stress tolerance or plant growth retardation under long-term drought stress?

Sreenivasulu, Nese; Harshavardhan, Vokkaliga T.; Govind, Geetha; Seiler, Christiane; Kohli, Ajay

doi:10.1016/j.gene.2012.06.076

Cited by 269 publications

(176 citation statements)

References 128 publications

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“…They are involved in cell organization, oxidative stress, photosynthesis, and defense responses. However, some of them are known to be regulated by ABA (Ton et al, 2009;Kar, 2011;Sreenivasulu et al, 2012;Tseng et al, 2013). It is possible that many of these genes in this group may be transcriptionally activated by ABA at a later time point or higher ABA concentration than we used.…”

Section: Discussionmentioning

confidence: 99%

The Arabidopsis NAC Transcription Factor ANAC096 Cooperates with bZIP-Type Transcription Factors in Dehydration and Osmotic Stress Responses

Xu¹,

Kim²,

Hyeon³

et al. 2013

The Plant Cell

239

177

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Multiple transcription factors (TFs) play essential roles in plants under abiotic stress, but how these multiple TFs cooperate in abiotic stress responses remains largely unknown. In this study, we provide evidence that the NAC (for NAM, ATAF1/2, and CUC2) TF ANAC096 cooperates with the bZIP-type TFs ABRE binding factor and ABRE binding protein (ABF/AREB) to help plants survive under dehydration and osmotic stress conditions. ANAC096 directly interacts with ABF2 and ABF4, but not with ABF3, both in vitro and in vivo. ANAC096 and ABF2 synergistically activate RD29A transcription. Our genome-wide gene expression analysis revealed that a major proportion of abscisic acid (ABA)-responsive genes are under the transcriptional regulation of ANAC096. We found that the Arabidopsis thaliana anac096 mutant is hyposensitive to exogenous ABA and shows impaired ABA-induced stomatal closure and increased water loss under dehydration stress conditions. Furthermore, we found the anac096 abf2 abf4 triple mutant is much more sensitive to dehydration and osmotic stresses than the anac096 single mutant or the abf2 abf4 double mutant. Based on these results, we propose that ANAC096 is involved in a synergistic relationship with a subset of ABFs for the transcriptional activation of ABA-inducible genes in response to dehydration and osmotic stresses.

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Section: Discussionmentioning

confidence: 99%

The Arabidopsis NAC Transcription Factor ANAC096 Cooperates with bZIP-Type Transcription Factors in Dehydration and Osmotic Stress Responses

Xu¹,

Kim²,

Hyeon³

et al. 2013

The Plant Cell

239

177

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show abstract

“…From a physiological perspective, it is well known that ABA accumulation in response to drought reduces transpirational water loss and helps plants survive, but under long-term stress, it is at the expense of photosynthetic productivity (Yoo et al, 2010). Hence, understanding the fine regulation of stomatal functions by ABA under short-term versus long-term stress is important for devising strategies to improve WUE and drought tolerance in crops (Sreenivasulu et al, 2012). There is clear evidence from studies on isolated leaf epidermis or guard cell protoplasts for the participatory role of ABA signaling complex in affording ABA sensitivity and reduction of water loss.…”

Section: Discussionmentioning

confidence: 99%

“…Overaccumulation of ABA or constitutive activation of ABA signaling reduces WUE and thus growth (Sreenivasulu et al, 2012;Pizzio et al, 2013;Zhao et al, 2013). For example, overexpression of NCED under the control of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit gene promoter, which is highly active in photosynthetic tissues, causes leaf yellowing, reduced chlorophyll content, and reduced growth rate (Gittins et al, 2000;Tung et al, 2008).…”

Section: Importance Of Aba Homeostasis In Improving Wue Under Long-tementioning

confidence: 99%

Abscisic Acid Flux Alterations Result in Differential Abscisic Acid Signaling Responses and Impact Assimilation Efficiency in Barley under Terminal Drought Stress

et al. 2014

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Abscisic acid (ABA) is a central player in plant responses to drought stress. How variable levels of ABA under short-term versus long-term drought stress impact assimilation and growth in crops is unclear. We addressed this through comparative analysis, using two elite breeding lines of barley (Hordeum vulgare) that show senescence or stay-green phenotype under terminal drought stress and by making use of transgenic barley lines that express Arabidopsis (Arabidopsis thaliana) 9-cis-epoxycarotenoid dioxygenase (AtNCED6) coding sequence or an RNA interference (RNAi) sequence of ABA 89-hydroxylase under the control of a droughtinducible barley promoter. The high levels of ABA and its catabolites in the senescing breeding line under long-term stress were detrimental for assimilate productivity, whereas these levels were not perturbed in the stay-green type that performed better. In transgenic barley, drought-inducible AtNCED expression afforded temporal control in ABA levels such that the ABA levels rose sooner than in wild-type plants but also subsided, unlike as in the wild type , to near-basal levels upon prolonged stress treatment due to down-regulation of endogenous HvNCED genes. Suppressing of ABA catabolism with the RNA interference approach of ABA 89-hydroxylase caused ABA flux during the entire period of stress. These transgenic plants performed better than the wild type under stress to maintain a favorable instantaneous water use efficiency and better assimilation. Gene expression analysis, protein structural modeling, and protein-protein interaction analyses of the members of the PYRABACTIN RESISTANCE1/PYRABACTIN RESISTANCE1-LIKE/REGULATORY COMPONENT OF ABA RECEPTORS, TYPE 2C PROTEIN PHOSPHATASE Sucrose nonfermenting1-related protein kinase2, and ABA-INSENSITIVE5/ABA-responsive element binding factor family identified specific members that could potentially impact ABA metabolism and stress adaptation in barley.Drought compromises grain yield in cereals, especially when the stress occurs during postanthesis (Boyer and Westgate, 2004;Sreenivasulu et al., 2007).In some crops, selection for and use of stay-green types has been promoted as a means for combating drought stress susceptibility (Thomas and Howarth, 2000). However, the molecular basis of stay-green phenotype in barley (Hordeum vulgare) has not been explored in detail. As the flag leaf is the principal source organ, sustaining its photosynthetic activity under postanthesis drought stress is considered a strategy to mitigate the yield penalty. Water use efficiency (WUE) is the amount of biomass (carbon) accumulated per unit of water, hence an important trait under water-limited conditions (Condon and Richards, 1992;Rebetzke et al., 2002;Richards et al., 2002). Breeding for WUE is considered important for developing drought-tolerant crops (Blum, 1996;Richards, 1996;Richards et al., 2002). Genetic loci that control transpiration efficiency have been identified (Teulat et al., 2002;Hall et al., 2005;Juenger et al., 2005). In Arabidopsis (Arabidopsis tha...

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“…ABA is also important for root growth and architectural modifications under nitrogen deficiency [9] and drought stress [10]. Furthermore, ABA is involved in the biosynthesis of dehydrins, osmoprotectants and protective proteins [3,11,12].…”

Section: Abscisic Acidmentioning

confidence: 99%

Introductory Chapter: Hormonal Regulation in Plant Development and Stress Tolerance

El‐Esawi¹

2017

Phytohormones - Signaling Mechanisms and Crosstalk in Plant Development and Stress Responses

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Contrapuntal role of ABA: Does it mediate stress tolerance or plant growth retardation under long-term drought stress?

Cited by 269 publications

References 128 publications

The Arabidopsis NAC Transcription Factor ANAC096 Cooperates with bZIP-Type Transcription Factors in Dehydration and Osmotic Stress Responses

The Arabidopsis NAC Transcription Factor ANAC096 Cooperates with bZIP-Type Transcription Factors in Dehydration and Osmotic Stress Responses

Abscisic Acid Flux Alterations Result in Differential Abscisic Acid Signaling Responses and Impact Assimilation Efficiency in Barley under Terminal Drought Stress

Introductory Chapter: Hormonal Regulation in Plant Development and Stress Tolerance

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