Contrast‐Based Real‐Time Assessment of Microcirculatory Changes in a Fatty Liver After Ischemia Reperfusion Injury

Kolachala, Vasantha L.; Jiang, Rong; Abramowsky, Carlos R.; Gupta, Nitika

doi:10.1097/mpg.0000000000001008

Cited by 12 publications

(6 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…blood flow velocity. Our estimate of about 25.65 μm s -1 is significantly lower compared to blood flow velocities observed by other research groups calculating capillary hemodynamic in the skin with velocities of around 192 μm s -1 [25] or maximal velocity of portal vein flow with 69.7 mm s -1 [26]. This difference may be explained by the fact that our measurements concerned blood flow in liver sinusoids which should have a significantly slower blood flow compared to dermal capillaries or the portal vein.…”

Section: Discussioncontrasting

confidence: 78%

Longitudinal imaging and femtosecond laser manipulation of the liver: How to generate and trace single-cell-resolved micro-damage in vivo

et al. 2020

View full text Add to dashboard Cite

The liver is known to possess extensive regenerative capabilities, the processes and pathways of which are not fully understood. A necessary step towards a better understanding involves the analysis of regeneration on the microscopic level in the in vivo environment. We developed an evaluation method combining longitudinal imaging analysis in vivo with simultaneous manipulation on single cell level. An abdominal imaging window was implanted in vivo in Balb/C mice for recurrent imaging after implantation. Intravenous injection of Fluorescein Isothiocyanate (FITC)-Dextran was used for labelling of vessels and Rhodamine 6G for hepatocytes. Minimal cell injury was induced via ablation with a femtosecond laser system during simultaneous visualisation of targeted cells using multiphoton microscopy. High-resolution imaging in vivo on single cell level including re-localisation of ablated regions in follow-up measurements after 2-7 days was feasible. Targeted single cell manipulation using femtosecond laser pulses at peak intensities of 3-6.6 μJ led to enhancement of FITC-Dextran in the surrounding tissue. These reactions reached their maxima 5-15 minutes after ablation and were no longer detectable after 24 hours. The procedures were well tolerated by all animals. Multiphoton microscopy in vivo, combined with a femtosecond laser system for single cell manipulation provides a refined procedure for longitudinal evaluation of liver micro-regeneration in the same region of interest. Immediate reactions after cell ablation and tissue regeneration can be analysed.

show abstract

Section: Discussioncontrasting

confidence: 78%

Longitudinal imaging and femtosecond laser manipulation of the liver: How to generate and trace single-cell-resolved micro-damage in vivo

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Otherwise, at 90 minutes after reperfusion in the control group, all of the US indices recovered to normal levels and were significantly different than the indices at 30 minutes after reperfusion. These findings illustrate that the hepatic artery may have undergone a transient spasm during hepatic IRI …”

Section: Discussionmentioning

confidence: 99%

Effect of Hepatic Artery Spasm on a Rat Model of Hepatic Ischemia‐Reperfusion Injury

Tang

et al. 2018

J of Ultrasound Medicine

View full text Add to dashboard Cite

Objectives To investigate hemodynamic changes in the hepatic artery after hepatic ischemia‐reperfusion injury (IRI) in rats via ultrasound (US) imaging and to discuss the protective effect of phentolamine (PHT) pretreatment on hepatic IRI. Methods Fifty rats were randomly divided into 3 groups: a sham operation group (n = 10), a control ischemia‐reperfusion group (n = 20), and a PHT pretreatment group (n = 20). Color Doppler flow imaging and contrast‐enhanced US examinations were performed in each group at 30 minutes (n = 10) and 90 minutes (n = 10) after reperfusion. Blood samples were obtained to analyze serum alanine aminotransferase and aspartate aminotransferase levels, and liver tissue specimens were collected for pathologic analysis. Results Using US, we found that hepatic artery resistance at 30 minutes after reperfusion in the control group was higher than that in the sham group (mean resistive index [RI] ± SD, 0.65 ± 0.09 versus 0.50 ± 0.09; P < .01), which was higher at 30 than 90 minutes (RI, 0.65 ± 0.09 versus 0.50 ± 0.08; P < .01) after reperfusion in the control group. However, the hepatic artery resistance and liver microcirculation in the PHT group were better than those in the control group at 30 minutes after reperfusion (RI, 0.54 ± 0.09 versus 0.65 ± 0.09; P < .05; time to peak, 31.94 ± 2.02 versus 48.34 ± 4.74 seconds; P < .01). Compared to the control group, the aspartate aminotransferase and alanine aminotransferase levels were significantly lower at 30 minutes after reperfusion in the PHT group (P < .05). A pathologic examination revealed a smaller hepatic artery diameter and a depressed vessel wall in the control group. Conclusions The hepatic artery can undergo a transient spasm during the hepatic IRI process, which can exacerbate liver damage. Phentolamine treatment can alleviate hepatic artery spasms, improve liver perfusion, and reduce liver injury by ameliorating the hepatic microcirculation.

show abstract

“…With increasing incidence of NAFLD, it is now being recognized that a steatotic liver when exposed to injury has a worse clinical outcome. (25)(26)(27)(28)(29)(30)(31)(32)(33)(34) Dynamics of microcirculation (35)(36)(37)(38) and ischemic injury (39) differ in steatotic liver compared to lean liver, implying that our current understanding of host response to injury in a "lean or normal" state does not necessarily apply fully to a steatotic liver. In this study, we show that CD8 1 cells are essential for the increased liver damage that occurs in steatotic liver during IRI compared with lean liver.…”

Section: Discussionmentioning

confidence: 99%

Loss of L‐selectin‐guided CD8+, but not CD4+, cells protects against ischemia reperfusion injury in a steatotic liver

et al. 2017

Self Cite

View full text Add to dashboard Cite

Background & Aims Steatotic liver responds with increased hepatocellular injury when exposed to an ischemic-reperfusion insult. Increasing evidence supports the role of immune cells as key mediators of this injury in a normal (lean) state, but data about their role in a steatotic liver are practically non-existent. The objective of the current study was to delineate contribution of specific phenotypes of T cells and adhesion molecules in exacerbated cell death in steatotic liver injury. Methods RNA sequencing was performed on isolated steatotic primary hepatocytes and T cell markers were assessed in hepatic lymphocytes after ischemia reperfusion injury (IRI) in high fat diet (HFD) fed mice. CD8−/− and CD4−/− mice along with CD8 and L-selectin antibody treated mice were fed on a HFD and hepatocellular injury was assessed by histology, propidium iodide injection and ALT after IRI. Results RNA sequencing demonstrated a strikingly differential gene profile in steatotic hepatocytes vs. lean hepatocytes. After injury, the HFD liver showed increased necrosis, infiltrating CD8+ cells, ALT and proinflammatory cytokines. Hepatic lymphocytes demonstrated increased CD8+/CD62L+(L-selectin) cells in HFD fed mice after IRI. CD8−/− mice and CD8 depleted C57BL/6 mice, demonstrated significant protection from injury, which was not seen in CD4−/− mice. L-selectin blockade also demonstrated significant hepatoprotection from IRI. L selectin ligand MECA-79 was increased in HFD fed mice undergoing IRI. Conclusion Blockade of CD8 and L-selectin but not CD4, ameliorated hepatocellular injury, confirming that CD8+ cells are critical drivers of injury in a steatotic liver. This represents a novel therapeutic target in steatotic liver injury, underlining the importance of development of therapies specific to a steatotic liver.

show abstract

Contrast‐Based Real‐Time Assessment of Microcirculatory Changes in a Fatty Liver After Ischemia Reperfusion Injury

Cited by 12 publications

References 21 publications

Longitudinal imaging and femtosecond laser manipulation of the liver: How to generate and trace single-cell-resolved micro-damage in vivo

Longitudinal imaging and femtosecond laser manipulation of the liver: How to generate and trace single-cell-resolved micro-damage in vivo

Effect of Hepatic Artery Spasm on a Rat Model of Hepatic Ischemia‐Reperfusion Injury

Loss of L‐selectin‐guided CD8+, but not CD4+, cells protects against ischemia reperfusion injury in a steatotic liver

Contact Info

Product

Resources

About