Contrast-enhanced ultrasound analysis of tissue perfusion in tumor-bearing mice following treatment with endostatin combined with radiotherapy

Ge, Wei; Yang, Zheng; Tao, Zezhang

doi:10.3892/etm.2014.1594

Cited by 6 publications

(6 citation statements)

References 20 publications

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“…Future investigations will focus on the correlation with urinary functionality evaluated by means of transurethral cystometry. Ultrasound could also be employed to assess the changes in vascularization using the contrast enhanced ultrasound (CEUS) technique (24)(25)(26), but ad hoc development of Tables Table 1. Results: the descriptive statistics refer to the mean bladder wall thickness normalized to the baseline value and is expressed in arbitrary units. P-values are reported as result of the Mann-Whitney-Wilcoxon test between groups.…”

Section: Resultsmentioning

confidence: 99%

A non-invasive ultrasound imaging method to measure acute radiation-induced bladder wall thickening in rats

Spinelli

Bresolin

Zuppone

et al. 2020

Preprint

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Background. Methods for the non-invasive quantification of changes in bladder wall thickness (BWT) as potential predictors of radiation cystitis (RC) in pre-clinical research would be desirable. The use of ultrasound (US) for this aim seems promising, but is still relatively unexplored. A method using US for BWT quantification in rats was developed and applied to measure early radiation-induced BWT changes. Methods. Two groups (n=9 each) of female Fischer rats were treated with a single radiation dose of 25-30 and 35-40Gy respectively, using an image-guided micro-irradiator; six untreated rats were monitored as a control group. Empty, half-filled and fully-filled bladder volumes were determined for four non-irradiated rats by measuring axes from US 3D-images and applying the ellipsoid formula. Mean BWT was estimated for both ventral and dorsal bladder sides through the measurement of the bladder wall area along a segment of 4mm in the central sagittal scan, in order to minimize operator-dependence on the measurement position. US acquisitions of all fully-filled rat bladders were also acquired immediately before, and 4 and 28 days after irradiation. Mean BWTs normalized to the baseline value and corrected for filling were then used to evaluate acute bladder wall thickening and to quantify the dose-effect. Results. The relationship between mean BWT and bladder volume in unirradiated rats showed that for a bladder volume >1.5mL the BWT is almost constant and equal to 0.30mm with variations within ±15%. The average ratios between BWT-post and BWT-pre irradiation showed a dose-effect relationship. Bladder wall thickening was observed for the 25-30 Gy and 35-40 Gy groups in 2/9 (22%) and 5/9 (56%) cases at day 4 and in 4/9 (44%) and 8/9 (89%) cases at day 28, respectively. The two groups showed significantly different BWTs both relative to the control group (p<0.0001) and between them (p=0.022). The BWT increment was on average 1.32±0.41, and was 1.30±0.21 after 25-30Gy and 1.47±0.29 and 1.90±0.83 after 35-40Gy at days 4 and 28 respectively. Conclusions. The feasibility of using US on a preclinical rat model to detect BWT changes after bladder irradiation was demonstrated, and a clear dose-effect relationship was quantified. Although preliminary, these results are promising in addressing the potential role of this non-invasive approach in quantifying RC.

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Section: Resultsmentioning

confidence: 99%

A non-invasive ultrasound imaging method to measure acute radiation-induced bladder wall thickening in rats

Spinelli

Bresolin

Zuppone

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…For example, in studies where ischemia was investigated in large parts of a tissue, the perfusion of the tissue (blood volume reaching a specific area) was measured via imaging techniques such as ultra-sound [43,44]. For applications that investigated small tissue areas, such as in the studies reported here, histological evaluation is the most common method of assessment of angiogenesis.…”

Section: Discussionmentioning

confidence: 98%

Multiple tissue response modifiers to promote angiogenesis and prevent the foreign body reaction around subcutaneous implants

Kastellorizios

Papadimitrakopoulos

Burgess

2015

Journal of Controlled Release

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“…Future investigations will focus on the correlation with urinary functionality evaluated by means of transurethral cystometry and also on the possible effect of isoflurane on rat bladder function by using different anesthesia agents like urethane [ 24 ]. Ultrasound could also be employed to assess the changes in vascularization using the contrast enhanced ultrasound (CEUS) technique [ 25 – 27 ], but ad hoc development of the animal model will be necessary. Finally a larger and blinded study including non-treated and radiated rats will be performed in order to demonstrate the reliability of the ultrasound BWT measurement method described in this work.…”

Section: Discussionmentioning

confidence: 99%

A non-invasive ultrasound imaging method to measure acute radiation-induced bladder wall thickening in rats

et al. 2020

View full text Add to dashboard Cite

Background Methods for the non-invasive quantification of changes in bladder wall thickness as potential predictors of radiation cystitis in pre-clinical research would be desirable. The use of ultrasound for this aim seems promising, but is still relatively unexplored. A method using ultrasound for bladder wall thickness quantification in rats was developed and applied to measure early radiation-induced bladder wall thickness changes. Methods Two groups (n = 9 each) of female Fischer rats were treated with a single radiation dose of 25–30 and 35–40 Gy respectively, using an image-guided micro-irradiator; six untreated rats were monitored as a control group. Empty, half-filled and fully-filled bladder volumes were determined for four non-irradiated rats by measuring axes from ultrasound 3D-images and applying the ellipsoid formula. Mean bladder wall thickness was estimated for both ventral and dorsal bladder sides through the measurement of the bladder wall area along a segment of 4 mm in the central sagittal scan, in order to minimize operator-dependence on the measurement position. Ultrasound acquisitions of all fully-filled rat bladders were also acquired immediately before, and 4 and 28 days after irradiation. Mean bladder wall thickness normalized to the baseline value and corrected for filling were then used to evaluate acute bladder wall thickening and to quantify the dose–effect. Results The relationship between mean bladder wall thickness and volume in unirradiated rats showed that for a bladder volume > 1.5 mL the bladder wall thickness is almost constant and equal to 0.30 mm with variations within ± 15%. The average ratios between post and pre irradiation showed a dose–effect relationship. Bladder wall thickening was observed for the 25–30 Gy and 35–40 Gy groups in 2/9 (22%) and 5/9 (56%) cases at day 4 and in 4/9 (44%) and 8/9 (89%) cases at day 28, respectively. The two groups showed significantly different bladder wall thickness both relative to the control group (p < 0.0001) and between them (p = 0.022). The bladder wall thickness increment was on average 1.32 ± 0.41, and was 1.30 ± 0.21 after 25–30 Gy and 1.47 ± 0.29 and 1.90 ± 0.83 after 35–40 Gy at days 4 and 28 respectively. Conclusions The feasibility of using ultrasound on a preclinical rat model to detect bladder wall thickness changes after bladder irradiation was demonstrated, and a clear dose–effect relationship was quantified. Although preliminary, these results are promising in addressing the potential role of this non-invasive approach in quantifying radiation cystitis.

show abstract

Contrast-enhanced ultrasound analysis of tissue perfusion in tumor-bearing mice following treatment with endostatin combined with radiotherapy

Cited by 6 publications

References 20 publications

A non-invasive ultrasound imaging method to measure acute radiation-induced bladder wall thickening in rats

A non-invasive ultrasound imaging method to measure acute radiation-induced bladder wall thickening in rats

Multiple tissue response modifiers to promote angiogenesis and prevent the foreign body reaction around subcutaneous implants

A non-invasive ultrasound imaging method to measure acute radiation-induced bladder wall thickening in rats

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