Contrasting activation energies of respiration and nutrient uptake drive lower ecosystem-level uptake at higher temperatures

Abstract

Abstract. Heterotrophic microbes play key roles in regulating fluxes of energy and nutrients, which are increasingly affected by globally changing environmental conditions such as warming and nutrient enrichment. While the effects of temperature and nutrients on microbial mineralization of carbon have been studied in some detail, much less attention has been given to how these factors are altering uptake rates of nutrients. We used laboratory experiments to simultaneously evaluate the temperature dependence of soluble reactive phosphorus (SRP) uptake and respiration by leaf litter-associated microbial communities from temperate headwater streams. Additionally, we evaluated the influence of the initial concentration of SRP on the temperature dependence of P uptake. Finally, we used simple simulation models to extrapolate our results and estimate the effect of warming and P availability on cumulative gross uptake at the ecosystem level. We found that the temperature dependence of P uptake was lower than that of respiration (0.48 vs. 1.02 eV). Further, the temperature dependence of P uptake increased with the initial concentration of SRP supplied, ranging from 0.12–0.48 eV over a 11–212 µg L-1 gradient in initial concentrations. Finally, despite our laboratory experiments showing increases in mass-specific rates of gross P uptake with temperature, our simulation models found declines in cumulative P uptake with warming because the increased rates of respiration at warmer temperatures more rapidly depleted benthic carbon substrates and consequently reduced the biomass of the benthic microbial community. Thus, even though mass-specific rates of P uptake were higher at the warmer temperatures, cumulative ecosystem-level P uptake was lower over the residence time of a pulsed input of organic carbon. Our results highlight the need to consider the combined effects of warming, nutrient availability, and resource availability/magnitude on carbon processing as important controls of nutrient processing.

Mechanistic constraints on the trade-off between photosynthesis and respiration in response to warming

“…The proposed mechanism relating energetic imbalances, differential thermal dependencies, and cell size is also consistent with experimental evidence that the thermal dependency of nutrient uptake is lower than that of both carbon fixation and growth (32,33). Similarly, isotope incorporation studies and enzyme assays showed that the thermal dependency of nitrate uptake by the diatom Thalassiosira pseudonana matched the thermal dependency of nitrate reductase activity, which was lower than that of growth (34).…”