Contrasting effects of heat shock during in vitro maturation on development of in vitro‐fertilized and parthenogenetic bovine embryos

Camargo, L. S. A.; Costa, Fabiano Freire; Munk, Michele; Wohlres-Viana, S.; Serapião, R. V.; Carvalho, Bruno Campos de; Campos-Júnior, Paulo Henrique Almeida; Vieira, Alex Cabral; Nogueira, Luiz Altamiro Garcia; Viana, J. H. M.

doi:10.1111/rda.13544

Cited by 7 publications

(4 citation statements)

References 45 publications

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“…The ovarian follicles and their enclosed oocytes are highly sensitive to hyperthermia (Roth, 2017;Islam et al, 2018). (Camargo et al, 2019) stated that heat shock increases the proportion of apoptotic oocytes during in vitro maturation (Camargo et al, 2019). The findings of a recent study suggest that camel oocytes are less tolerant of the short acute heat shock, which is linked with reduced ooplasm diameter and maturation rate as well as increased incidence of chromosomal count abnormalities (Islam et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Effect of Heat Stress on Developmental Competence of In Vitro Matured Oocytes of Camelus Dromedaries with Different Qualities

Ashour¹,

El-Sayed²,

Khalifa³

et al. 2020

WVJ

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The deleterious effect of heat stress on cumulus-oocytes complexes (COCs) competence is well recognized in different livestock species. Therefore, the present study aimed to investigate the effect of physiologically relevant heat stress on the developmental competence of camel COCs during in vitro maturation (IVM). A total of 1548 COCs were divided into six groups in this study. The groups were named K1 and K2 representing good and low-quality COCs incubated at 38.5oC for 30 hours. While K3 and k4 represent good and low-quality COCs exposed to 41oC for the first 6 hours of IVM. Finally, K5 and k6 represent the groups of good and low-quality COCs exposed to 42oC for the first 6 hours of IVM. After exposure of COCs to heat stress at 41°C and 42°C during the first 6 hours of in vitro maturation, the COCs were incubated at 38.5°C for 24 hours of IVM. The in vitro matured COCs were activated to cleave using ethanol followed by 4 mM 6-DMAP and developed embryos were cultured in vitro for 7 days post parthenogenetic activation. The results of this study indicated that heat stress at 42oC significantly decreased the Pb (polar body) extrusion rate in K4 and K6, compared to other groups. Additionally, the embryo cleavage rate was significantly lower for good and low-quality oocytes exposed to heat stress (K2, K3, K4, K5, and K6), compared to good quality COCs of the control group (K1). The cleavage rate was lower for low quality (K2; 63 ± 1.28) than good quality COCs (K1; 53 ± 1.85). The percentages of oocytes that developed to the blastocyst stage were lower for K2, K3, K4, K5, and K6 than K1. Moreover, the blastocyst rate was lower for K2 (9 ± 0.22) than K1 (15 ± 0.22). The results of this study indicated that exposure of camel oocytes to heat stress for 6 hours during in vitro maturation severely reduced extrusion of polar body, cleavage, and blastocyst rates. The low-quality camel COCs were reduced developmental capacity than good quality oocytes.

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Section: Introductionmentioning

confidence: 99%

Effect of Heat Stress on Developmental Competence of In Vitro Matured Oocytes of Camelus Dromedaries with Different Qualities

Ashour¹,

El-Sayed²,

Khalifa³

et al. 2020

WVJ

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“…Heat shock proteins have been identified as diagnostic, prognostic, and therapeutic biomarkers in human cancer [11]. Heat shock protein 70 (Hsp70) is the most structurally and functionally conserved protein in this family of proteins [12]. Hsp70 is a highly conserved functional protein across species [13].…”

Section: Introductionmentioning

confidence: 99%

Resveratrol Reduces Kidney Injury in a Rat Model of Uremia and is Associated with Increased Expression of Heat Shock Protein 70 (Hsp70)

et al. 2020

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This study was funded by the Yantaishan Hospital and The Project of Yantai Science and Technology (No. 2017WS112) Background:This study aimed to investigate the effects of resveratrol on kidney function in a rat model of uremia and the expression of heat shock proteins. Material/Methods:The rat model of uremia was developed by 5/6 nephrectomy of Sprague-Dawley rats. The Hsp70 inhibitor MKT-077, a rhodacyanine dye, was used. The study groups included rats with sham surgery (the sham group), the rat model of uremia (the model group), the solvent-treated control group (the control group), the rat model treated with resveratrol group (the resveratrol group), the rat model treated with MKT-077 (the MKT-077 group), and the resveratrol+MKT-077 group. Kidney tissues were studied histologically. Renal cell apoptosis was detected by the TUNEL method. Expression of p53, Bax, and Bcl-2 mRNA and protein were detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunohistochemistry, respectively. Results:Compared with the sham group, the expression levels of heat shock proteins Hsp70, Hsp90, Hsp27, Hsp25, Hsp40, and Hsp60 in the kidney of the rat model group increased to different degrees. Compared with the model group, the Hsp70 levels in the resveratrol group were significantly increased (p<0.05). Compared with the model group, treatment with MKT-077 reduced the survival rate of rats, which was increased following resveratrol treatment. Compared with the resveratrol group, renal function in the resveratrol+MKT-077 group was significantly reduced (p<0.05). Conclusions:In a rat model of uremia, resveratrol reduced renal injury and improved both renal function and survival, which were associated with increased expression of Hsp70.

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“…Для получения партеногенов млекопитающих используют модель экстракорпорального созревания донорских ооцитов с последующей их активацией. Существуют множество типов активации яйцеклеток, в том числе химическая, физическая, в итоге приводящие к подъему концентрации интрацитоплазматического кальция, как следствие, стартуют процессы, приводящие к партеногенетическому развитию [3], [4], [8], [16]. Однако до сих пор оптимальной модели активации ооцитов коров к партеногенезу не разработано.…”

Section: постановка проблемы (Introduction)unclassified

Partenogenetic development of Bos taurus embryos from oocytes matured in different culture systems

Kuzmina¹

2020

Agrarian Bulletin of The

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Abstract. Identification of the factors determining of donor’s oocyte competence to parthenogenetic development will allow developing an effective method for obtaining parthenotes to solve fundamental problems of regulating gene activity in ontogenesis, creating homozygous embryonic stem cell lines, improving the stages of cloning technology, and modeling of in vitro oocyte maturation media. The purpose of study is to evaluate the potencies of Bos taurus oocytes matured in different culture systems to cold shock-induced parthenogenesis. Methods. For oocyte maturation, culture systems of the following composition were used: 1 – TC-199 with 10 % fetal bovine serum (FBS), 50 μg/ml estradiol, 10 μg/ml luteinizing hormone, 10 μg/ml follicle-stimulating hormone; 2 – TC-199 with 10 % estrous serum of cows; 3 – TC-199 with 50 % fluid from follicles (Ø 3–8 mm); 4 – TC-199 with 50 % protein of follicular fluid (molecular weight of 65 kDa); 5 – TC-199 with 10 % FBS, 1×106 granulosa cells/ml medium; 6 – TC-199 with 10 % FBS and walls of follicles (Ø 6–8 mm); 7 – TC-199 with 10 % FBS, 1×106 granulosa cells/ml medium and walls of follicles (Ø 6–8 mm). After 24 hours of cultivation, the oocytes were activated by cold shock (exposure time 20 minutes, temperature 0…–4 °C. Results. The proportion of embryos at the stages of late morula and blastocysts from oocytes matured in system 7 was 45 % (58/129), which was significantly higher than in other systems: 1 – 28 % (39/141), P < 0.05; 2 – 31 % (42/137), P < 0.05; 3 – 25 % (33/133), P < 0.01; 4 – 18 % (25/139), P < 0.001; 5 – 31 % (41/132), P < 0.05; 6 – 33 % (43/129). The introduction of estradiol or structural components of antral follicles into the oocytes maturation medium contributed to an increase in the proportion of parthenotes at the preimplantation stages of development, including blastocysts, and a decrease in the level of degenerated embryos. Scientific novelty. A comparative morphological analysis of the potentials for parthenogenesis of bovine oocytes matured in various culture systems and activated by cold shock was carried out for the first time. Optimal systems for in vitro maturation of female gametes were proposed. Based on the analysis of the results, we recommend before induction to parthenogenesis bovine oocytes culture in media supplemented with 50 ng/ml estradiol or structural components of antral follicles producing estradiol.

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Contrasting effects of heat shock during in vitro maturation on development of in vitro‐fertilized and parthenogenetic bovine embryos

Cited by 7 publications

References 45 publications

Effect of Heat Stress on Developmental Competence of In Vitro Matured Oocytes of Camelus Dromedaries with Different Qualities

Effect of Heat Stress on Developmental Competence of In Vitro Matured Oocytes of Camelus Dromedaries with Different Qualities

Resveratrol Reduces Kidney Injury in a Rat Model of Uremia and is Associated with Increased Expression of Heat Shock Protein 70 (Hsp70)

Partenogenetic development of Bos taurus embryos from oocytes matured in different culture systems

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