Contribution au dosage de la choline dans le sérum sanguin

Wachsmuth, H; Koeckhoven, L. Van

doi:10.1016/0009-8981(59)90131-7

Cited by 4 publications

(2 citation statements)

References 7 publications

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“…Naturally occurring quaternary ammonium compounds, such as choline chloride and other choline derivatives, have been determined in animal tissues (Wacsmuth and Van Koeckhoven, 1959;Vogel and Debusses, 1962) by colorimetric methods using iodine (Hayashi et al, 1962), dye indicators (Auerbach, 1943), or ammonium reineckate as a reagent (Salwin et al, 1958). These methods, in addition to their nonspecificity, lacked the sensitivity desired for a residue method.…”

mentioning

confidence: 99%

Plant-growth regulant. Determination of chlorocholine chloride residues in wheat grain, straw, and green wheat foliage

Mooney¹,

Pasarela²

1967

J. Agric. Food Chem.

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A method is presented for the chromatographic separation and subsequent colorimetric measurement of chlorocholine chloride (CCC) at residue levels in wheat grain and plants at various stages of growth. Chlorocholine chloride is removed from tissue background by adsorption chromatography on aluminum oxide, and measured colorimetrically as a dipicrylamine-chlorocholine chloride complex at 415 mg. The average recoveries of CCC from wheat grain, green wheat foliage, and corresponding wheat straw were 76, 86.4, and 90%, respectively, with average control values of 0.10, 0.16, and 0.27 p.p.m. A rate-of-disappearance study at a treatment level of 4 pounds of CCC per acre of wheat showed a biological half life for CCC of 13 days. Gycocel[chlorocholine chloride (CCC), 2-chloroethyl trimethylammonium chloride] is a plant growth regulant. The physiological and economic importance Dipicrylamine Reagent (2,2 ',4,4',6, (Eastman Chemical Co.). Prepare a 0.01 % solution in purified methylene chloride.Aluminum Oxide. Fisher Scientific Co., Catalog No. A-540, adsorption alumina for chromatographic analysis.Amberlite LA-2 Liquid Anion Exchange Resin (Rohm and Haas Co., Philadelphia, Pa.). Prepare a 10% solution in carbon tetrachloride.Cycocel (2-Chloroethyl Trimethyl ammonium Chloride) Standard (American Cyanamid Co., Princeton, N. J.). Prepare standard solutions in methanol containing 10 and 1.0 µg. of CCC per ml. of solution.

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confidence: 99%

Plant-growth regulant. Determination of chlorocholine chloride residues in wheat grain, straw, and green wheat foliage

Mooney¹,

Pasarela²

1967

J. Agric. Food Chem.

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“…The second group of methods implements the precipitation of choline with potassium triiodide, and either the determination of liberated iodine (Stanȇk, 1905;Sharpe, 1923;Hayashi, Unemoto & Miyaki, 1962), or the redissolution and subsequent photometric determination of choline triiodide (Appleton et al, 1953). The third group of methods is based on the reaction of choline with phosphormolybdic acid (Wheeldon & Collins, 1958;Wachsmuth & Van Koeckhoven, 1959). The fourth group of methods uses enzymatic oxidation of choline by choline oxidase, and the subsequent determination of generated hydrogen peroxide (Rahimi & Joseph, 2019).…”

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confidence: 99%

Spectrophotometric determination of L-α-glycerylphosphorylcholine in pharmaceutical formulations and industrial equipment cleaning rinse water with the WAKO Phospholipids C assay kit

Nikolaychuk¹

2023

PeerJ Analytical Chemistry

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A simple spectrophotometric method for the determination of L-α-glycerylphosphorylcholine in pharmaceutical formulations and industrial equipment cleaning rinse water using the enzyme glycerophosphocholine phosphodiesterase and the WAKO Phospholipids C assay kit was proposed. The method is based on the enzymatic hydrolysis of α-GPC to choline by glycerophosphocholine phosphodiesterase, the reaction of choline with the components of the assay kit, and the colourimetric determination of the formed product. The calibration graph is linear in the range from 1 to 40 mg/l of α-GPC, the molar attenuation coefficient is 1,110 m2/mol, the limit of detection is 1 mg/l, the limit of quantification is 3.3 mg/l, the method is selective with respect to the common excipients, shows a good accuracy (the relative uncertainty does not exceed 7%) and precision (the relative standard deviation does not exceed 5.5%), does not require lengthy sample preparation and sophisticated laboratory equipment and is suitable for the routine analysis of pharmaceutical formulations and industrial equipment cleaning rinse water.

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Progress in biochemical investigations. I. Nitrogen compounds: 1959

Natelson

1960

Microchemical Journal

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Contribution au dosage de la choline dans le sérum sanguin

Cited by 4 publications

References 7 publications

Plant-growth regulant. Determination of chlorocholine chloride residues in wheat grain, straw, and green wheat foliage

Plant-growth regulant. Determination of chlorocholine chloride residues in wheat grain, straw, and green wheat foliage

Spectrophotometric determination of L-α-glycerylphosphorylcholine in pharmaceutical formulations and industrial equipment cleaning rinse water with the WAKO Phospholipids C assay kit

Progress in biochemical investigations. I. Nitrogen compounds: 1959

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