Contribution of 1p, 19q, 9p and 10q Automated Analysis by FISH to the Diagnosis and Prognosis of Oligodendroglial Tumors According to WHO 2016 Guidelines

Michaud, Karine; Tayrac, Marie de; D’Astous, Myreille; Duval, Céline; Paquet, Claudie; Samassékou, Oumar; Gould, Peter; Saïkali, Stéphan

doi:10.1371/journal.pone.0168728

Cited by 8 publications

(9 citation statements)

References 46 publications

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“…The major aim of this study was to continue our previous work [ 1 ] on the prognostic value of chromosome 9p status in anaplastic oligodendrogliomas (OIII) and to confirm the reliability of the FISH technique using a standard FISH platform, an easily available commercial probe and an automated software analysis package with a previously established algorithm [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Impact of 9p deletion and p16, Cyclin D1, and Myc hyperexpression on the outcome of anaplastic oligodendrogliomas

et al. 2018

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ObjectiveTo study the presence of 9p deletion and p16, cyclin D1 and Myc expression and their respective diagnostic and prognostic interest in oligodendrogliomas.MethodsWe analyzed a retrospective series of 40 consecutive anaplastic oligodendrogliomas (OIII) from a single institution and compared them to a control series of 10 low grade oligodendrogliomas (OII). Automated FISH analysis of chromosome 9p status and immunohistochemistry for p16, cyclin D1 and Myc was performed for all cases and correlated with clinical and histological data, event free survival (EFS) and overall survival (OS).ResultsChromosome 9p deletion was observed in 55% of OIII (22/40) but not in OII. Deletion was highly correlated to EFS (median = 29 versus 53 months, p<0.0001) and OS (median = 48 versus 83 months, p<0.0001) in both the total cohort and the OIII population. In 9p non-deleted oligodendrogliomas, p16 hyperexpression correlated with a shorter OS (p = 0.02 in OII and p = 0.0001 in OIII) whereas lack of p16 expression was correlated to a shorter EFS and OS in 9p deleted OIII (p = 0.001 and p = 0.0002 respectively). Expression of Cyclin D1 was significantly higher in OIII (median expression 45% versus 14% for OII, p = 0.0006) and was correlated with MIB-1 expression (p<0.0001), vascular proliferation (p = 0.002), tumor necrosis (p = 0.04) and a shorter EFS in the total cohort (p = 0.05). Hyperexpression of Myc was correlated to grade (median expression 27% in OII versus 35% in OIII, p = 0.03), and to a shorter EFS in 9p non-deleted OIII (p = 0.01).ConclusionChromosome 9p deletion identifies a subset of OIII with significantly worse prognosis. The combination of 9p status and p16 expression level identifies two distinct OIII populations with divergent prognosis. Hyperexpression of Bcl1 and Myc appears highly linked to anaplasia but the prognostic value is unclear and should be investigated further.

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Section: Introductionmentioning

confidence: 99%

Impact of 9p deletion and p16, Cyclin D1, and Myc hyperexpression on the outcome of anaplastic oligodendrogliomas

et al. 2018

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“…It is known that 30-70 % of OAs are characterized by LOH 1p and LOH 19q mutations, i.e. genetic features of oligodendroglioma [7,9]. At the same time, 30 % of OAs are associated with gene TP 53 or LOH 17p mutations -signs of astrocytoma [4,19].…”

Section: Discussionmentioning

confidence: 99%

“…At the present, OA as an independent tumor type is confirmed by the achievements of immunohistochemistry and genetics [6][7][8]. However, even the results of these studies cannot explain OA origin and its histological features [4,6,9]. The long-term neurosurgical experience has shown that OA treatment remains one of still unclarified problems in oncology [10,11].…”

Section: N3mentioning

confidence: 95%

“…It is known that the deep expansion of OA, especially in the central brain structures, in combination with the histological features and infiltrative nature of the tumor, adversely impact the treatment results [12]. Insufficient knowledge of OAs topography limits the surgical treatment potential or leads to unreasoned total resection with damage of eloquent cortical areas and central brain structures [9,12,13]. This fact can cause neurological deficiency and irreversible disorders, which significantly affect the results of OAs treatment in general, worsening the early results and quality of life and life expectancy [13,14].…”

Section: N3mentioning

confidence: 99%

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Heterogeneity of oligoastrocytoma: morphology, surgery, and survival in the series of 163 patients. Retrospective study

Kliuchka

Rozumenko

et al. 2018

Ukr Neurosurg J

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Introduction. The current approach to oligoastrocytoma (OA) treatment includes surgery taking into account the anatomical and physiological accessibility using various radio-and chemotherapy protocols. Nevertheless, it should be recognized that influence of OA histological structure on the surgery of these tumors has not conclusively established.Objective. To improve diagnostic and surgical tactics in oligoastrocytoma by comparing determined clinical-histological patterns. Materials and methods.Retrospective clinical-morphological comparison and analysis of treatment results in 163 patients with OA were performed taking into account histological structure. OAII (WHO) was diagnosed in 32 patients (19.6 %), 131 patients (80.4 %) developed OAIII (WHO). Results. In 52 OA cases (32 %) oligodendroglial component (oOA) prevailed, in 48 OA cases (29 %) astrocytic component (aOA) prevailed, in 63 OA cases (39 %) there was relatively equal cells distribution of both components (оаОА). The surgical treatment of 163 patients included the following: gross total removal, 60 patients (31.4 %); subtotal removal, 98 patients (60.2 %); and partial removal, 4 patients (2.4 %). A total of 106 (65.0 %) patients presented with Karnofsky Performance Status Scale (KPS) ≥ 80. One hundred and fifty-three patients (93.9 %) experienced postsurgery KPS ≥ 80. There was no perioperative death. All 163 patients received radiation therapy and 87 patients (53.4 %) received chemotherapy as well. A significant difference (p< 0.05) in overall survival (OS) was found between surgery results of OA histological groups. Clinical and MRI/CT findings significantly correlated with histological types of OA as well as results of treatment: the overall survival in patients with oOA was 100.5 ± 4.6 months, aOA -48.2 ± 4.5 months, oaOA -76.6 ± 4.9 months, averaging 49.9 ± 2.4 months. Conclusions.Diagnosing, surgery, and survival of OA are determined by the uniqueness of the histological structure of these tumors, the interaction of their components, topography and expansion direction. The key to successful results is a differential approach to planning diagnostic tactics, method of removal and management in late post-op period.

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“…As there are contradictive reports that the 2016-WHO defined genetic entities in some cases do not circumscribe with morphological features as proposed by WHO system [40]; therefore, WHO-2016 system needs to be validated rigorously for analysing defined genetic entities concordance with phenotypic/pathologic analysis. While considering ODs; the segmental chromosomal loss of 1p and 19q by fluorescence in situ hybridization (FISH) [293,294], may contribute to diagnosis and prognosis of ODs in accordance with WHO-2016 guidelines. In pertinent to examining segmental chromosomal losses by FISH and NGS, it has been proposed that NGS is superior to FISH in distinguishing segmental chromosomal losses from whole-arm deletions [295].…”

Section: Future Perspectivesmentioning

confidence: 99%

Differential mRNA and miRNA expression in oligodendrogliomas of different grades of malignancy

Nawaz¹

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All praise to Allah Almighty, the most beneficent, the most merciful, Who honoured man with intellectual power and spiritual insight to understand, discover and to conquer wondrous mysteries. Countless salutations upon the Holy Prophet Muhammad (peace be upon him), for enlightening humanity with the essence of faith and guiding the mankind to the true path of life. I would like to express my profound gratitude to all those who helped me in different ways during my PhD. In particular I am rather indebted to thank; Luciano Neder: PhD supervisor, for giving me opportunity to work in his group. His kind supervision, guidance, and personal interests in my research work made this all possible. What most profoundly I recognize is that, he gave me freedom, letting me doing things independently with my own ideas and decisions. I took advantage of this freedom for writing fellowship applications independently, travel grants for participating in conferences and writing up scientific articles. Collectively this all made my way to academic growth as an independent researcher. I have really enjoyed our chats and gossips not only about science, but about everything in life; family, culture and social norms across the borders. Many thanks for your support through difficult circumstances that had arisen throughout my stay at Ribeirao Preto. I really had an amazing time with you. Hadi Valadi: my co-supervisor, who proved to be one of the productive additions in my scientific career. It was a great experience being at Gothenburg and enjoying exosomes science in his lab at Sahlgrenska Academy. He provided me the facilities of the lab in a proper, suitable and helping manner and this was the first time I gained very authoritative knowledge about newly described area of exosomes research. This is safe to say that, it will come in very useful way in my future career. Last, but not least, my affiliations with him recognize his patronizing concerns towards scientific goals. There was so much to learn from you. I thank Tao Jin at the Department of Rheumatology and Inflammation Research at Sahlgrenska Academy for his cooperation and support. And "wow" all those guys at department lunch room with whom I have had company during lunch time.

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Contribution of 1p, 19q, 9p and 10q Automated Analysis by FISH to the Diagnosis and Prognosis of Oligodendroglial Tumors According to WHO 2016 Guidelines

Cited by 8 publications

References 46 publications

Impact of 9p deletion and p16, Cyclin D1, and Myc hyperexpression on the outcome of anaplastic oligodendrogliomas

Impact of 9p deletion and p16, Cyclin D1, and Myc hyperexpression on the outcome of anaplastic oligodendrogliomas

Heterogeneity of oligoastrocytoma: morphology, surgery, and survival in the series of 163 patients. Retrospective study

Differential mRNA and miRNA expression in oligodendrogliomas of different grades of malignancy

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