Contribution of biofilm formation genetic locus, <i>pgaABCD</i>, to antibiotic resistance development in gut microbiome

Lin, Dachuan; Chen, Kaichao; Guo, Jiubiao; Ye, Lianwei; Li, Ruichao; Chan, Edward Wai Chi; Chen, Sheng

doi:10.1080/19490976.2020.1842992

Cited by 13 publications

(6 citation statements)

References 36 publications

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“…The potential biological mechanisms on how the electroacupuncture treatment could inhibit the peripheral and central nerve system inflammatory response by balancing the gut microbiota, including brain-gut microbiome-based neuroimmune [ 86 , 87 ], RNA modification [ 88 , 89 ], and genetic basis [ 90 , 91 ]. There were three important aspects in this process.…”

Section: Discussionmentioning

confidence: 99%

Electroacupuncture could balance the gut microbiota and improve the learning and memory abilities of Alzheimer’s disease animal model

et al. 2021

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Alzheimer’s disease (AD), as one of most common dementia, mainly affects older people from the worldwide. In this study, we intended to explore the possible mechanism of improving cognitive function and protecting the neuron effect by electroacupuncture. Method: We applied senescence-accelerated mouse prone 8 (SAMP8) mice as AD animal model, used Morris water maze, HE staining, 16S rDNA amplicon sequencing of gut microbiota and ELISA to demonstrate our hypothesis. Results: electroacupuncture improved the learning and memory abilities in SAMP8 mice (P<0.05) and could protect the frontal lobe cortex and hippocampus of SAMP8 mice; electroacupuncture significantly decreased the expression of IL-1β (P<0.01), IL-6 (P<0.01) and TNF-α (P<0.01 in hippocampus, P<0.05 in serum) in serum and hippocampus; electroacupuncture balanced the quantity and composition of gut microbiome, especially of the relative abundance in Delta-proteobacteria (P<0.05) and Epsilon-proteobacteria (P<0.05). Conclusion: electroacupuncture treatment could inhibit the peripheral and central nerve system inflammatory response by balancing the gut microbiota.

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Section: Discussionmentioning

confidence: 99%

Electroacupuncture could balance the gut microbiota and improve the learning and memory abilities of Alzheimer’s disease animal model

et al. 2021

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“…Group of monobactam antibiotics aztreonam) showed the highest activity towards biofilm producers. Rubab and Oh 28 concluded that Shiga toxin-producing E. coli (STEC) exhibited the highest resistance to erythromycin; Lin et al 29 suggested that biofilm-producing genes in E. coli are responsible for higher resistance against antibiotic drugs; they observed that strains that carry pgaABCD and hipAB exhibited higher resistance. Current findings suggest that high antibiotic resistance in biofilm-producing E. coli leads to many bacterial water-borne diseases.…”

Section: Discussionmentioning

confidence: 99%

Isolation and identification of Biofilm-producing E. coli from drinking water.

Fatima

Mirani

Siddiqui

et al. 2022

Int. j. endorsing health sci. res.

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Background: The increasing rate of water-borne diseases in Karachi demands the characterization of associated pathogens. Nowadays, water-borne infections become more resistant to treatment due to biofilm formation and excessive use of antibiotics. Biofilm formation in water deteriorates the quality of water. This study identified and characterized biofilm-producing E. coli in drinking water. Methodology: Water samples were analyzed for heterotrophic plate count and total coliforms, fecal coliforms, and E. coli. The Kirby disk bar diffusion method was used to investigate antibiotic susceptibility in biofilm-producing E. coli. The Congo red and tube ring methods were used to identify biofilm producers. The effect of biofilm formation on the hydrophobicity of E. coli was performed by the BATH method. The soft agar method determines the colony spreading ability of biofilm and non-biofilm producers. Molecular characterization of virulence genes of E. coli was performed by a PCR. Scanning electron microscopy for biofilm construction was conducted. Results: The total 120 water samples were tested for heterotrophic plate count and total coliforms, fecal coliforms, and E. coli. 78% were unfit in this study, and 21.66% were fit. 38 E. coli strains were found in water samples. According to findings, the hydrophobicity of biofilm-producing isolates increased with the incubation period. Colony-forming unit drops one logarithm in the biofilm state compared to the planktonic stage. Biofilm producers were more resistant to antibiotics. The virulence genes, pet, lt, and stx2, were used for the molecular characterization. Conclusion: The presence of biofilm-producing E. coli in drinking water is alarming, and it indicates inappropriate treatment of the water supply system. To prevent rapid water-borne diseases, adequate actions are required to control drinking-water biofilm producers.

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“…To characterize this optrA -positive isolate, we used Illumina Hiseq platform to sequence the whole genome of E. faecium SZ21B15. Genomic DNA extraction, library preparation, and sequencing were performed as previously described ( 8 , 9 ). A total of 224 contigs (>200 bp) (GenBank accession on.…”

Section: Observationmentioning

confidence: 99%

Bile Carriage of optrA -Positive Enterococcus faecium in a Patient with Choledocholith

et al. 2023

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Oxazolidinone are effective antimicrobial agents for the treatment of infections caused by multidrug-resistant Gram-positive bacteria, including vancomycin-resistant enterococci. The emergence and global spread of transferable oxazolidinone resistance genes such as optrA is worrisome. Enterococcus spp. can become causes of hospital-associated infections and are also widely distributed in the gastrointestinal tracts of animals and the natural environment.

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Contribution of biofilm formation genetic locus, pgaABCD, to antibiotic resistance development in gut microbiome

Cited by 13 publications

References 36 publications

Electroacupuncture could balance the gut microbiota and improve the learning and memory abilities of Alzheimer’s disease animal model

Electroacupuncture could balance the gut microbiota and improve the learning and memory abilities of Alzheimer’s disease animal model

Isolation and identification of Biofilm-producing E. coli from drinking water.

Bile Carriage of optrA -Positive Enterococcus faecium in a Patient with Choledocholith

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