Contribution of Bone Marrow–derived Cells to Reparative Dentinogenesis Using Bone Marrow Transplantation Model

Frozoni, Marcos; Marques, Marcelo Rocha; Hamasaki, Sílvia Kaoru; Mohara, Nelson Tsutomu; Soares, Adriana de Jesus; Zaia, Alexandre Augusto

doi:10.1016/j.joen.2019.12.003

Cited by 8 publications

(4 citation statements)

References 38 publications

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“…In addition, it is a terminal differentiation marker of odontoblasts [35]. Recently, Frozoni used BMMSCs in pulp cap in a mouse model and they found that GFP labelled-BMMSCs were directly utilised and GFP-labelled DSP positive odontoblasts were observed in the root canal 7 weeks later [36]. This study may shed some light on how to explain their observations.…”

Section: Discussionmentioning

confidence: 86%

Exosomes Derived from Stem Cells from the Apical Papilla Promote Dentine-Pulp Complex Regeneration by Inducing Specific Dentinogenesis

XueYing

Jiang

et al. 2020

Stem Cells International

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Regenerative endodontic procedures (REPs) are a new option for the treatment of dental pulp or periapical diseases in permanent teeth with open apices. Histologically, the new tissues formed in the root canal after REPs are mainly cementum- or bone-like mineralised tissues, but not the real dentine-pulp complex. Therefore, how to promote dentine-pulp complex regeneration and improve the clinical effects of REPs has become a prominent research topic. Stem cells from apical papilla (SCAP) are derived from the dental papilla that can differentiate into primary odontoblasts and dental pulp cells that produce root dentine and dental pulp. Exosomes are the key regulator for the paracrine activity of stem cells and can influence the function of recipient cells. In this study, SCAP-derived exosomes (SCAP-Exo) were introduced into the root fragment containing bone marrow mesenchymal stem cells (BMMSCs) and transplanted subcutaneously into immunodeficient mice. We observed that dental pulp-like tissues were present and the newly formed dentine was deposited onto the existing dentine in the root canal. Afterwards, the effects of SCAP-Exo on the dentinogenesis of BMMSCs were elucidated in vitro. We found that the gene and protein expression of dentine sialophosphoprotein and mineralised nodule formation in BMMSCs treated with SCAP-Exo were significantly increased. In summary, SCAP-Exo were endocytosed by BMMSCs and obviously improved their specific dentinogenesis. The use of exosomes derived from dental stem cells could comprise a potential therapeutic approach for dentine-pulp complex regeneration in REPs.

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Section: Discussionmentioning

confidence: 86%

Exosomes Derived from Stem Cells from the Apical Papilla Promote Dentine-Pulp Complex Regeneration by Inducing Specific Dentinogenesis

XueYing

Jiang

et al. 2020

Stem Cells International

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“…They mainly focused on the mineralizing potential and the interfacial interaction of the materials with host subcutaneous tissues, which are not specific to clinical use and were excluded. Four studies focused on the effect of ethylene diamine tetracetic acid (EDTA; Liu et al, 2021), bone marrow‐derived cells (Frozoni et al, 2020), a triple antibiotic paste (Quispe‐Salcedo et al, 2020) or sclerostin (Frozoni et al, 2020) for endodontic use were also excluded. Furthermore, three studies which assessed the involvement of glucose supply during pulpal wound healing (Tohma et al, 2020), evaluated the bone tissue reaction of hydraulic cements in femur of rats (Quintana et al, 2019) or focused on the validation of an in vivo animal for furcal perforation (Silva et al, 2009) are not within scope of this review.…”

Section: Resultsmentioning

confidence: 99%

Present status and future directions: Hydraulic materials for endodontic use

Camilleri

Atmeh

et al. 2022

Int Endodontic J

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Background Hydraulic materials are used in Endodontics due to their hydration characteristics namely the formation of calcium hydroxide when mixing with water and also because of their hydraulic properties. These materials are presented in various consistencies and delivery methods. They are composed primarily of tricalcium and dicalcium silicate, and also include a radiopacifier, additives and an aqueous or a non‐aqueous vehicle. Only materials whose primary reaction is with water can be classified as hydraulic. Objectives Review of the classification of hydraulic materials by Camilleri and the literature pertaining to specific uses of hydraulic cements in endodontics namely intra‐coronal, intra‐radicular and extra‐radicular. Review of the literature on the material properties linked to specific uses providing the current status of these materials after which future trends and gaps in knowledge could be identified. Methods The literature was reviewed using PUBMED, and for each clinical use, the in vitro properties such as physical, chemical, biological and antimicrobial characteristics and clinical data were extracted and evaluated. Results A large number of publications were retrieved for each clinical use and these were grouped depending on the property type being investigated. Conclusions The hydraulic cements have made a difference in clinical outcomes. The main shortcoming is the poor testing methodologies employed which provide very limited information and also inhibits adequate clinical translation. Furthermore, the clinical protocols need to be updated to enable the materials to be employed effectively.

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“…Использование этой группы материалов в эндодонтии демонстрирует высокую эффективность применения: при покрытии пульпы, пульпотомии, пломбировании апикальной трети корневых каналов, закрытии перфораций в корневых каналах, регенеративном эндодонтическом лечении. Существуют многочисленные исследования in vitro и in vivo, описывающие и подтверждающие физико-химические и биологические свойства этой группы биокерамики [10,26,32,39,67,70,87]. Минерал триоксид агрегат (MTA) был первым биокерамическим материалом, успешно применяемым в эндодонтии [44,47].…”

Section: таблица 1 механизм действия лекарственных средств на пульпу ...unclassified

Современные Лекарственные Средства Для Эндодонтического Лечения Зубов У Детей

Шаковец¹,

Романова²

2021

Стоматология. Эстетика. Инновации

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За последнее десятилетие подходы к эндодонтическому лечению зубов у детей претерпели значительные изменения. Приоритетным направлением являются методы, способствующие регенерации пульпы и/или ее максимальному сохранению. Для этой цели разрабатываются лекарственные средства, обладающие подобными свойствами.Цель. Провести обзор литературы по современным методам эндодонтического лечения зубов у детей и используемым лекарственным средствам.Материалы и методы. Проведен обзор 98 научных статей, посвященных современным методам эндодонтического лечения зубов у детей и описанию достоинств и недостатков используемых при этом лекарственных средств. Представлена классификация лекарственных средств по механизму действия на пульпу зуба. Подробно описаны материалы на основе биокерамики, их состав и способы применения. Over the past decade, endodontic treatment in children has undergone significant changes. Priority methods are those that promote pulp regeneration and / or maximum preservation. For this purpose, medicaments with similar properties are being developed.Purpose: to review the literature on modern endodontic treatment methods in children and modern medicaments.Materials and methods. A review of 98 scientific articles devoted to modern pulp therapy methods and a description of the advantages and disadvantages of medicaments used in their implementation is carried out. The classification of materials according to the mechanism of action on the tooth pulp is presented. Bioceramics based materials, their composition and methods of application are described in detail.

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Contribution of Bone Marrow–derived Cells to Reparative Dentinogenesis Using Bone Marrow Transplantation Model

Cited by 8 publications

References 38 publications

Exosomes Derived from Stem Cells from the Apical Papilla Promote Dentine-Pulp Complex Regeneration by Inducing Specific Dentinogenesis

Exosomes Derived from Stem Cells from the Apical Papilla Promote Dentine-Pulp Complex Regeneration by Inducing Specific Dentinogenesis

Present status and future directions: Hydraulic materials for endodontic use

Современные Лекарственные Средства Для Эндодонтического Лечения Зубов У Детей

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