Contribution of Caspase-8 Genotypes to Colorectal Cancer Risk in Taiwan

Abstract

Background/Aim: The aim of this study was to examine the role of caspase-8 rs3834129 polymorphism on colorectal cancer (CRC) risk in Taiwanese CRC patients and healthy controls. Materials and Methods: The caspase-8 rs3834129 (-652 6N insertion/deletion) polymorphic genotypes were analyzed in 362 patients with CRC and the same number of age-and gender-matched healthy subjects. The interaction of caspase-8 rs3834129 genotypes with personal behaviors and clinicopathological features were also examined. Results: The percentage of variants ID and DD for caspase-8 rs3834129 genotype were 37.6 and 5.8% in CRC group and 39.0 and 6.6% in the control group, respectively (p for trend=0.7987). The allelic frequency distribution analysis showed that caspase-8 rs3834129 D allele conferred a non-significant lower susceptibility for CRC compared with I allele (OR=0.92, p=0.5063). There was no obvious link between caspase-8 rs3834129 genotype and CRC risk among ever-smokers, non-smokers, non-alcohol drinkers or alcohol drinkers. No statistically significant correlation was observed between caspase-8 rs3834129 genotypic distribution and age, gender, tumor size, location or metastasis status. Conclusion: Overall, caspase-8 rs3834129 genotypes may not serve as predictors for CRC risk or prognosis.

Association of Caspase-8 Genotypes With Bladder Cancer Risk

“…Biologically, the del genotype has been reported to destroy a stimulatory protein 1 binding element in the promoter regulatory region, leading to decreased caspase-8 transcriptional activity and eventually suppression of T lymphocyte apoptosis (45). There were also some studies reporting no association between the genotypes of caspase-8 rs3834129 with various types of cancer, for instance, the oral and colorectal cancers in the Taiwanese population (31,48). This is the first study evaluating caspase-8 rs3834129 polymorphism in bladder cancer susceptibility in the Taiwanese population, however no significant association ANTICANCER RESEARCH 39: 4767-4773 (2019) 4770 was found.…”

Contribution ofMatrix Metalloproteinase-1Genotypes to Colorectal Cancer in Taiwan

“…Collection of 362 CRC cases and 362 controls. The investigated population has been recruited as described in our previous studies (9)(10)(11)(12). Concisely, CRC cases have been recruited at the outpatient clinics of general surgery by well-trained colleagues.…”

“…MMP-1 rs1799750 genotyping methodology. The genomic DNA from peripheral blood leukocytes of all participants were extracted and stored at -80˚C as previously published (9,10). The MMP-1 rs1799750 genotyping methodology is the same as previously reported (17,19).…”

Significant Association Between the MiR146a Genotypes and Susceptibility to Childhood Acute Lymphoblastic Leukemia in Taiwan

“…DNA isolation. Genomic DNA from the peripheral blood samples of each participant was extracted, measured by the spectrophotometer, aliquoted, and stored at -20˚C prior to use or at -80˚C for a longer period as we performed routinely (24)(25)(26).…”