Contribution of CE to the Analysis of Protein or Peptide Biomarkers

Mesbah, Kiarach; Verpillot, Romain; l’Escaille, François de; Falmagne, Jean Bernard; Taverna, Myriam

doi:10.1007/978-1-62703-296-4_13

Cited by 6 publications

(5 citation statements)

References 41 publications

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“…Protein electrophoresis is an important laboratory technique, which is conventionally used for the screening of protein abnormalities in serum as well as in other biological fluids such as urine and cerebrospinal fluid (CSF) (1)(2). The recent development of CE techniques suitable for routine laboratory use has represented a major breakthrough in the separation and quantification of protein fractions, since this technique allows to obtain highly accurate results, contextually saving precious human and economical resources (5).…”

Section: Discussionmentioning

confidence: 99%

“…Suggestive variations in the relative concentration of protein fractions also allow reliable screening and monitoring of pathological dis orders associated with nephrotic syndrome, inflammatory and hepatic diseases (1,2). Capillary electrophoresis (CE) is a relatively innovative technique, whose diffusion in clinical laboratories has remarkably increased over the past decade (3).…”

Section: Introductionmentioning

confidence: 99%

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Analytical Evaluation of the Novel Helena V8 Capillary Electrophoresis System

Lippi¹,

Battistelli²,

Vernocchi³

et al. 2013

Journal of Medical Biochemistry

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SummaryBackground: Capillary electrophoresis (CE) is used for the screening of protein abnormalities in serum and other biological fluids. Since the Helena V8 CE system has been recently commercialized, the aim of this study was to assess its analytical performance under routine laboratory conditions. Methods: The evaluation of Helena V8 (Medical Systems S.p.A., Genova, Italy) for protein fractions (i.e., albumin, alpha-1, alpha-2, beta-1, beta-2, gamma, albumin/globulin ratio [A/G]) and monoclonal component (MC) quantification included imprecision studies, linearity, and comparison with Sebia Capillarys (Sebia, Evry Cedex, France), which was considered the reference analyzer. Results: The imprecision of Helena V8 on all parameters was comprised between 0.8% and 15.2%; the linearity was excellent up to an MC value of 14.5% (r=1.00). The comparison studies showed excellent Spearman's correlations with Sebia Capillarys, with coefficients comprised between 0.84 and 0.99 (all p<0.001). The mean bias of Helena V8 versus Sebia Capillarys was -0.02% for G/A, -0.2% for albumin, 2.1% for alpha-1, 0.1% for alpha-2, 0.3% for beta-1, -0.2% for beta-2, -2.2% for gamma and 1 g/L for MC. Conclusions: The results of this evaluation attest that Helena V8 is a suitable alternative for routine separation and quantification of protein fractions and monoclonal component.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analytical Evaluation of the Novel Helena V8 Capillary Electrophoresis System

Lippi¹,

Battistelli²,

Vernocchi³

et al. 2013

Journal of Medical Biochemistry

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“…The interest on the use of CE for the analysis of peptides and proteins has continued in the last years . Most of the methodological developments in CE for the analysis of peptides and proteins are directed to prevent their adsorption on the inner capillary wall, and to increase their sensitivity.…”

Section: Peptides and Proteinsmentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

et al. 2015

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This review work presents and discusses the main applications of capillary electromigration methods in food analysis and Foodomics. Papers that were published during the period February 2013-February 2015 are included following the previous review by Garcia-Cañas et al. (Electrophoresis, 2014, 35, 147-169). Analysis by CE of a large variety of food-related molecules with different chemical properties, including amino acids, hazardous amines, peptides, proteins, phenols, polyphenols, lipids, carbohydrates, DNAs, vitamins, toxins, contaminants, pesticides, residues, food additives, as well as small organic and inorganic compounds. This work includes recent results on food quality and safety, nutritional value, storage, bioactivity, as well as applications of CE for monitoring food processing. The use, among other CE developments, of microchips, CE-MS, and chiral CE in food analysis and Foodomics is also discussed.

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“…CE‐LIF detection represents one of the most sensitive techniques for the quantitative analysis of proteins and peptides. In the field of clinical diagnosis and clinical chemical analysis, this technique offers also a good separation power and is therefore particularly adapted to analyze biological samples, where micro‐ and nanomolar protein concentrations are common . However, many peptides and proteins do not exhibit a sufficient native fluorescence due to the lack or low quantity of amino acids exhibiting UV‐absorbing moieties (i.e., Trp, Tyr, and/or Phe residues).…”

Section: Introductionmentioning

confidence: 99%

Derivatization strategies for CE‐LIF analysis of biomarkers: Toward a clinical diagnostic of familial transthyretin amyloidosis

et al. 2014

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We report three derivatization strategies for CE analysis with LIF detection (CE-LIF) of two synthetic peptides mimicking the wild and mutated fragments of interest for the diagnosis of familial transthyretin amyloidosis. The precapillary derivatization of the peptides with three optical tags, 5-carboxytetramethylrhodamin succinimidyl ester (TAMRA-SE), naphtalene-2,3-dicarboxyaldehyde (NDA), and 3-(2-furoyl)quinoline-2-carboxyaldehyde (FQ) has been investigated by CE-LIF detection and MS. Results provide evidence that high reaction yields have been reached whereas the multitagging phenomenon has occurred for both NDA and TAMRA-SE labeling procedures. The derivatization and electrokinetic separation of a mixture of the two peptides of interest for the pathology diagnosis (22-aa peptides that differ only from one amino acid) were achieved using both approaches. The highest resolution with a value of 2.5 was obtained with TAMRA-SE labeled derivatives whereas NDA gave the best detection sensitivity (LOD of 2.5 μM). The validation of the developed methods showed a good linearity (R ≥ 0.997) between the peak area of the labeled derivatives and the peptide concentration for both NDA and FQ labeling procedures. The intraday RSDs of A and the migration times were less than 3.8 and 2.2%, respectively.

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Contribution of CE to the Analysis of Protein or Peptide Biomarkers

Cited by 6 publications

References 41 publications

Analytical Evaluation of the Novel Helena V8 Capillary Electrophoresis System

Analytical Evaluation of the Novel Helena V8 Capillary Electrophoresis System

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

Derivatization strategies for CE‐LIF analysis of biomarkers: Toward a clinical diagnostic of familial transthyretin amyloidosis

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