Contribution of different biosynthetic pathways to species selectivity of aminoglycerophospholipids assembled into mitochondrial membranes of the yeast Saccharomyces cerevisiae

Bürgermeister, Maria; Birner-Grünberger, Ruth; Heyn, M. F.; Daum, Günther

doi:10.1016/j.bbalip.2004.09.005

Cited by 30 publications

(23 citation statements)

References 52 publications

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“…This interpretation is in line with a previous report showing that two different enzyme activities are responsible for the synthesis of alk-1-enyl-acyl-PE and diacyl-PE in rabbit heart membranes (41). Separate pools of PE consisting of different molecular species have also been reported in other mammalian cells (42) and yeast (43).…”

Section: Discussionsupporting

confidence: 81%

Phosphatidylethanolamine in Trypanosoma brucei Is Organized in Two Separate Pools and Is Synthesized Exclusively by the Kennedy Pathway

Signorell

Rauch

Jelk

et al. 2008

Journal of Biological Chemistry

104

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Phosphatidylethanolamine is a major phospholipid class of all eukaryotic cells. It can be synthesized via the CDP-ethanolamine branch of the Kennedy pathway, by decarboxylation of phosphatidylserine, or by base exchange with phosphatidylserine. The contributions of these pathways to total phosphatidylethanolamine synthesis have remained unclear. Although Trypanosoma brucei, the causative agent of human and animal trypanosomiasis, has served as a model organism to elucidate the entire reaction sequence for glycosylphosphatidylinositol biosynthesis, the pathways for the synthesis of the major phospholipid classes have received little attention. We now show that disruption of the CDP-ethanolamine branch of the Kennedy pathway using RNA interference results in dramatic changes in phosphatidylethanolamine, phosphatidylserine, and phosphatidylcholine. By targeting individual enzymes of the pathway, we demonstrate that de novo phosphatidylethanolamine synthesis in T. brucei procyclic forms is strictly dependent on the CDPethanolamine route. Interestingly, the last step in the Kennedy pathway can be mediated by two separate activities leading to two distinct pools of phosphatidylethanolamine, consisting of predominantly alk-1-enyl-acyl-or diacyl-type molecular species. In addition, we show that phosphatidylserine in T. brucei procyclic forms is synthesized exclusively by base exchange with phosphatidylethanolamine.

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Section: Discussionsupporting

confidence: 81%

Phosphatidylethanolamine in Trypanosoma brucei Is Organized in Two Separate Pools and Is Synthesized Exclusively by the Kennedy Pathway

Signorell

Rauch

Jelk

et al. 2008

Journal of Biological Chemistry

104

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“…These studies revealed that the proportions of PC produced by the two routes are similar in total cellular membranes, microsomes, and mitochondrial membranes, suggesting that PC is evenly distributed from a single pool [42,43]. Consistent with this notion, the molecular species profiles of PC in different subcellular membranes are similar [44,45].…”

Section: Two Biosynthetic Pathwayssupporting

confidence: 69%

“…PE is for the most part synthesized by mitochondrial Psd1p and by Psd2p in the Golgi/vacuole [17,23], and its conversion to PC requires intermembrane transport to the ER (reviewed in [52]. Consistent with the above notion, a recent analysis of the species distributions of the aminoglycerophospholipids in yeast subcellular fractions did not reveal species-selectivity in interorganelle phospholipid transport [45]. The preferential methylation of 32:2 PE is for the most part conferred by Cho2p with a minor contribution by Opi3p [51].…”

Section: Species-selectivity Of the Pc Biosynthetic Enzymessupporting

confidence: 58%

“…Pulse labeling with stable isotope labeled L-serine of psd1 and psd2 mutants and analysis of the labeled PE species synthesized by ESI-MS/MS could shed a light on the possibility that differences in species composition between the PE pools produced by Psd1p and Psd2p account for the preferred methylation of PE derived from Psd2p. The similarity in PE species profiles between psd1 and psd2 strains [45] seems to argue against this possibility.…”

Section: Species-selectivity Of the Pc Biosynthetic Enzymesmentioning

confidence: 84%

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Metabolism of phosphatidylcholine and its implications for lipid acyl chain composition in Saccharomyces cerevisiae

Kroon

2007

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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Phosphatidylcholine (PC) is a very abundant membrane lipid in most eukaryotes including the model organism Saccharomyces cerevisiae. Consequently, the molecular species profile of PC, i.e. the ensemble of PC molecules with acyl chains differing in number of carbon atoms and double bonds, is important in determining the physical properties of eukaryotic membranes, and should be tightly regulated. In this review current insights in the contributions of biosynthesis, turnover, and remodeling by acyl chain exchange to the maintenance of PC homeostasis at the level of the molecular species in yeast are summarized. In addition, the phospholipid class-specific changes in membrane acyl chain composition induced by PC depletion are discussed, which identify PC as key player in a novel regulatory mechanism balancing the proportions of bilayer and nonbilayer lipids in yeast.

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“…Myo-inositol and ethanolamine were direct precursors of phosphatidylinositol (PI) and phosphatidylethanolamine (PE) in S. cerevisiae, respectively (Bürgermeister et al, 2004). It was reported that phospholipids such as PI and PE played important roles during adaptation of S. cerevisiae to unfavorable environmental conditions (Mannazzu et al, 2008;Xia and Yuan, 2009).…”

Section: Inoculum Size-dependent Metabolic Regulationmentioning

confidence: 98%

Inoculum size-dependent interactive regulation of metabolism and stress response of Saccharomyces cerevisiae revealed by comparative metabolomics

Ding

Tian

Cheng

et al. 2009

Journal of Biotechnology

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Contribution of different biosynthetic pathways to species selectivity of aminoglycerophospholipids assembled into mitochondrial membranes of the yeast Saccharomyces cerevisiae

Cited by 30 publications

References 52 publications

Phosphatidylethanolamine in Trypanosoma brucei Is Organized in Two Separate Pools and Is Synthesized Exclusively by the Kennedy Pathway

Phosphatidylethanolamine in Trypanosoma brucei Is Organized in Two Separate Pools and Is Synthesized Exclusively by the Kennedy Pathway

Metabolism of phosphatidylcholine and its implications for lipid acyl chain composition in Saccharomyces cerevisiae

Inoculum size-dependent interactive regulation of metabolism and stress response of Saccharomyces cerevisiae revealed by comparative metabolomics

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