Contribution of Drosophila DEG/ENaC Genes to Salt Taste

Liu, Lei; Leonard, A. Soren; Motto, David G.; Feller, Margaret A.; Price, Margaret P.; Johnson, Wayne; Welsh, Michael J.

doi:10.1016/s0896-6273(03)00394-5

Cited by 166 publications

(210 citation statements)

References 67 publications

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“…2 B), and the behavioral and electrophysiological responses to water were not different among all DGs␣ strains examined in this study. It is known that salt responses in larvae require amiloride-sensitive channels encoded by ppk11 and ppk19 (Liu et al, 2003), and the low and high concentrations of salt responses do not require G␥1 in adult flies (Ishimoto et al, 2005). These findings together with our results suggest that DGs␣ in non-Gr5a GRNs serves for other signaling than taste or that the non-Gr5a GRNs containing DGs␣ are mechanosensory neurons.…”

Section: Discussionsupporting

confidence: 73%

Gs Is Involved in Sugar Perception in Drosophila melanogaster

Ueno

Kohatsu

Clay

et al. 2006

Journal of Neuroscience

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In Drosophila melanogaster, gustatory receptor genes (Grs) encode G-protein-coupled receptors (GPCRs) in gustatory receptor neurons (GRNs) and some olfactory receptor neurons. One of the Gr genes, Gr5a, encodes a sugar receptor that is expressed in a subset of GRNs and has been most extensively studied both molecularly and physiologically, but the G-protein ␣ subunit (G␣) that is coupled to this sugar receptor remains unknown. Here, we propose that Gs is the G␣ that is responsible for Gr5a-mediated sugar-taste transduction, based on the following findings: First, immunoreactivities against Gs were detected in a subset of GRNs including all Gr5a-expressing neurons. Second, trehalose-intake is reduced in flies heterozygous for null mutations in DGs␣, a homolog of mammalian Gs, and trehalose-induced electrical activities in sugar-sensitive GRNs were depressed in those flies. Furthermore, expression of wild-type DGs␣ in sugar-sensitive GRNs in heterozygotic DGs␣ mutant flies rescued those impairments. Third, expression of double-stranded RNA for DGs␣ in sugarsensitive GRNs depressed both behavioral and electrophysiological responses to trehalose. Together, these findings indicate that DGs␣ is involved in trehalose perception. We suggest that sugar-taste signals are processed through the Gs␣-mediating signal transduction pathway in sugar-sensitive GRNs in Drosophila.

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Section: Discussionsupporting

confidence: 73%

Gs Is Involved in Sugar Perception in Drosophila melanogaster

Ueno

Kohatsu

Clay

et al. 2006

Journal of Neuroscience

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“…In addition, at least three members of the TRP channels, TRP1, Painless, and TRPL are expressed in bitter neurons in the labellum where they are involved in detection of aversive compounds (Al-Anzi et al, 2006;Kim et al, 2010;Zhang et al, 2013b). Also, several ppk channels are expressed in taste neurons where they are required for relevant taste modalities such as low-salt detection (Liu et al, 2003b) and intraspecific chemical communication in larvae (Mast et al, 2014), and water perception (Cameron et al, 2010) and chemical communication during courtship in adults Lu et al, 2012;Starostina et al, 2012;Thistle et al, 2012;Toda et al, 2012;Vijayan et al, 2014). Finally, DmXR, a receptor homologous to metabotropic glutamate receptors that has lost the ability to bind glutamate (Mitri et al, 2004), may also act as a taste receptor.…”

Section: Chemoreceptors and Gustatory Detectionmentioning

confidence: 99%

“…Some of the proteins listed here have not been confirmed as bona fide receptors yet (see main text for details). #In larvae, low-salt solutions seem to be detected by ppk11 and ppk19 (Liu et al, 2003b). *In larvae, GR43a is the only sugar receptor (Mishra et al, 2013).…”

Section: Male Pheromonementioning

confidence: 99%

Chemicals and chemoreceptors: ecologically relevant signals driving behavior in Drosophila

2015

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Insects encounter a vast repertoire of chemicals in their natural environment, which can signal positive stimuli like the presence of a food source, a potential mate, or a suitable oviposition site as well as negative stimuli such as competitors, predators, or toxic substances reflecting danger. The presence of specialized chemoreceptors like taste and olfactory receptors allows animals to detect chemicals at short and long distances and accordingly, trigger proper behaviors toward these stimuli. Since the first description of olfactory and taste receptors in Drosophila melanogaster 15 years ago, our knowledge on the identity, properties, and function of specific chemoreceptors has increased exponentially. In the last years, multidisciplinary approaches combining genetic tools with electrophysiological techniques, behavioral recording, evolutionary analysis, and chemical ecology studies are shedding light on our understanding on the ecological relevance of specific chemoreceptors for the survival of Drosophila in their natural environment. In this review we discuss the current knowledge on chemoreceptors of both the olfactory and taste systems of the fruitfly. We focus on the relevance of particular receptors for the detection of ecologically relevant cues such as pheromones, food sources, and toxic compounds, and we comment on the behavioral changes that the detection of these chemicals induce in the fly. In particular, we give an updated outlook of the chemical communication displayed during one of the most important behaviors for fly survival, the courtship behavior. Finally, the ecological relevance of specific chemicals can vary depending on the niche occupied by the individual. In that regard, in this review we also highlight the contrast between adult and larval systems and we propose that these differences could reflect distinctive requirements depending on the change of ecological niche occupied by Drosophila along its life cycle.

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“…Pickpocket (ppk), the first subunit to be identified, is involved in locomotive behaviour in fruit fly larvae. Other ppk genes have been implicated in fluid re-distribution (Adams et al, 1998;Liu et al, 2003a), gustatory salt perception (Liu et al, 2003b), and male response to female pheromones . Preliminary analysis of the pea aphid genome has indicated that there are between 23 and 26 ppk genes present with conserved structural features, but low overall sequence similarity.…”

Section: Sodium Channel 60ementioning

confidence: 99%

Identification of ion channel genes in the Acyrthosiphon pisum genome

Dale

Jones

Tamborindeguy

et al. 2010

Insect Molecular Biology

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Abstracti mb_975 141..154Aphids are major pests of crops, causing hundreds of millions of dollars worth of damage annually. Ion channel proteins are often the targets of modern insecticides and mutations in ion channel genes can lead to resistance to many leading classes of insecticides. The sequencing of the pea aphid, Acyrthosiphon pisum, genome has now allowed detailed in silico analysis of the aphid ion channels. The study has revealed significant differences in the composition of the ion channel families between the aphid and other insects. For example A. pisum does not appear to contain a homologue of the nACh receptor alpha 5 gene whilst the calcium channel beta subunit has been duplicated. These variations could result in differences in function or sensitivity to insecticides. The genome sequence will allow the study of aphid ion channels to be accelerated, leading to a better understanding of the function of these economically important channels. The potential for identifying novel insecticide targets within the aphid is now a step closer.

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Contribution of Drosophila DEG/ENaC Genes to Salt Taste

Cited by 166 publications

References 67 publications

Gs Is Involved in Sugar Perception in Drosophila melanogaster

Gs Is Involved in Sugar Perception in Drosophila melanogaster

Chemicals and chemoreceptors: ecologically relevant signals driving behavior in Drosophila

Identification of ion channel genes in the Acyrthosiphon pisum genome

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