Contribution of glibenclamide-sensitive, ATP-dependent K+ channel activation to acetophenone analogues-mediated in vitro pulmonary artery relaxation of rat

Seto, Sai Wang; Ho, Yick Yan; Hui, Hung Ngai; Au, Alice Lai Shan; Kwan, Yiu Wa

doi:10.1016/j.lfs.2005.05.063

Cited by 10 publications

(10 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…H 2 S can open K ATP channels in the cell membrane of aortic vascular smooth muscle, causing cytomembrane hyperpolarization. The K ATP channel is very important in the cardiovascular system [35][36][37][38][39][40][41] and H 2 S acts as an endogenous K ATP channel opener. K ATP channels are recognized for their cardioprotective role in ischemia [35] .…”

Section: Discussionmentioning

confidence: 99%

“…The K ATP channel is very important in the cardiovascular system [35][36][37][38][39][40][41] and H 2 S acts as an endogenous K ATP channel opener. K ATP channels are recognized for their cardioprotective role in ischemia [35] . Evidence suggests that Kir6.1 and SUR2B are the main K ATP channel subunits expressed in the vascular smooth muscle [42][43][44] .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The vasorelaxing effect of hydrogen sulfide on isolated rat aortic rings versus pulmonary artery rings

et al. 2011

View full text Add to dashboard Cite

Aim:To compare the vasorelaxing effects of hydrogen sulfide (H 2 S) on isolated aortic and pulmonary artery rings and to determine their action mechanisms. Methods: H 2 S-induced vasorelaxation of isolated rat aortic versus pulmonary artery rings under 95% O 2 and 5% CO 2 was analyzed. The expression of cystathinonine gamma-lyase (CSE), cystathionine beta synthase (CBS), 3-mercaptopyruvate sulfurtransferase (3MST), SUR2B and Kir6.1 was examined. Results: NaHS caused vasorelaxation of rat aortic and pulmonary artery rings in a dose-dependent manner. NaHS dilated aortic rings to a greater extent (16.4%, 38.4%, 64.1%, 84.3%, and 95.9% at concentrations of 50, 100, 200, 500, and 1000 μmol/L, respectively) than pulmonary artery rings (10.1%, 22.2%, 50.6%, 73.6%, and 84.6% at concentrations of 50, 100, 200, 500 and 1000 μmol/L, respec tively). The EC 50 of the vasorelaxant effect for aortic rings was 152.17 μmol/L, whereas the EC 50 for pulmonary artery rings was 233.65 μmol/L. The vasorelaxing effect of H 2 S was markedly blocked b y cellular and mitochondrial membrane K ATP channel blockers in aortic rings (P<0.01). In contrast, only the cellular membrane K ATP channel blocker inhibited H 2 S-induced vasorelaxation in pulmonary artery rings. SUR2B mRNA and protein expression was higher in aortic rings than in pulmonary artery rings. Cystathinonine gamma-lyase (CSE) but not cystathionine beta synthase (CBS) expression in aortic rings was higher than in pulmonary artery rings. 3-Mercapto pyruvate sulfurtransferase (3MST) mRNA was lower in aortic rings than in pulmonary artery rings. Conclusion: The vasorelaxing effect of H 2 S on isolated aortic rings was more pronounced than the effect on pulmonary artery rings at specific concentrations, which might be associated with increased expression of the K ATP channel subunit SUR2B.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The vasorelaxing effect of hydrogen sulfide on isolated rat aortic rings versus pulmonary artery rings

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Solutions (external bathing solutions and pipette solutions) and voltage protocols used for whole-cell recordings of BK Ca and K ATP channels were similar to our previous reports [24,25].…”

Section: Isolation Of Aortic Smooth Muscle Cells and Patch-clamp Elecmentioning

confidence: 96%

“…Rat aorta (endothelium denuded) smooth muscle cells were enzymatically dissociated (using collagenase and papain) with methods as reported previously by our group [23][24][25] for single-cell patch-clamp electrophysiology experiments. Solutions (external bathing solutions and pipette solutions) and voltage protocols used for whole-cell recordings of BK Ca and K ATP channels were similar to our previous reports [24,25].…”

Section: Isolation Of Aortic Smooth Muscle Cells and Patch-clamp Elecmentioning

confidence: 99%

Formononetin, an isoflavone, relaxes rat isolated aorta through endothelium-dependent and endothelium-independent pathways

Wu¹,

Wu³

et al. 2010

The Journal of Nutritional Biochemistry

View full text Add to dashboard Cite

We evaluated the vasorelaxation effects of formononetin, an isoflavone/phytoestrogen found abundantly in Astragalus mongholicus Bunge, on rat isolated aorta and the underlying mechanisms involved. Cumulative administration of formononetin, genistein, daidzein and biochanin A relaxed phenylephrine-preconstricted aorta. Formononetin and biochanin A caused a similar magnitude of relaxation whereas daidzein was least potent. Mechanical removal of endothelium, L-NAME (100 microM) and methylene blue (10 microM) suppressed formononetin-induced relaxation. Formononetin increased endothelial nitric oxide (NO) synthase (eNOS), but not inducible NO synthase, activity with an up-regulation of eNOS mRNA and p-eNOS(Ser1177) protein expression. In endothelium-denuded preparations, formononetin-induced vasorelaxation was significantly reduced by glibenclamide (3 microM) and iberiotoxin (100 nM), and a combination of glibenclamide (3 microM) plus iberiotoxin (100 nM) abolished the relaxation. In contrast, formononetin-elicited endothelium-independent relaxation was not altered by ICI 182,780 (10 microM, an estrogen receptor (ER alpha/ER beta) antagonist) or mifepristone (10 microM, a progesterone receptor antagonist). In single aortic smooth muscle cells, formononetin caused opening of iberiotoxin-sensitive Ca(2+)-activated K(+) (BK(Ca)) channels and glibenclamide-sensitive adenosine triphosphate (ATP)-dependent K(+) (K(ATP)) channels. Thus, our results suggest that formononetin caused vascular relaxation via endothelium/NO-dependent mechanism and endothelium-independent mechanism which involves the activation of BK(Ca) and K(ATP) channels.

show abstract

“…In the second and third groups, propranolol, a non-selective ␤-adrenergic receptor blocker, 1 M (Seto et al, 2006) and atropine, a muscarinic receptor blocker, 10 M (Gonzáles-Luis et al, 2007) were added for 5 min, respectively, before the tissue was precontracted again by phenylephrine.…”

Section: Experimental Protocolmentioning

confidence: 99%

Thymoquinone-induced relaxation of isolated rat pulmonary artery

Suddеk

2010

Journal of Ethnopharmacology

View full text Add to dashboard Cite

Contribution of glibenclamide-sensitive, ATP-dependent K+ channel activation to acetophenone analogues-mediated in vitro pulmonary artery relaxation of rat

Cited by 10 publications

References 21 publications

The vasorelaxing effect of hydrogen sulfide on isolated rat aortic rings versus pulmonary artery rings

The vasorelaxing effect of hydrogen sulfide on isolated rat aortic rings versus pulmonary artery rings

Formononetin, an isoflavone, relaxes rat isolated aorta through endothelium-dependent and endothelium-independent pathways

Thymoquinone-induced relaxation of isolated rat pulmonary artery

Contact Info

Product

Resources

About