Contribution of
            <i>kasA</i>
            Analysis to Detection of Isoniazid-Resistant
            <i>Mycobacterium tuberculosis</i>
            in Singapore

Lee, Ann S. G.; Lim, Irene H. K.; Tang, Lynn L. H.; Telenti, Amalio; Wong, Serre-Yu

doi:10.1128/aac.43.8.2087

Cited by 114 publications

(41 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These strains might have additional mutations in other codons of the amplified region of katG gene or outside this investigated region or in other genes involved in INH resistance. Furthermore, INH resistance could be due to unknown mechanisms (Lee et al, 1999).…”

Section: Resultsmentioning

confidence: 99%

Molecular analysis of multidrug resistantMycobacterium tuberculosis isolates from Morocco

Sabouni¹,

Kourout

Chaoui

et al. 2008

Ann. Microbiol.

View full text Add to dashboard Cite

Tuberculosis remains a global threat to public health. Considerable efforts have been made to combat this disease. However, the emergence of Mycobacterium tuberculosis (Mtb) strains resistant to the major anti-tuberculosis drugs especially multidrug resistant (MDR) strains poses a deadly threat to control programs. The present study aims to identify the most common mutations within multidrug-resistant M. tuberculosis Moroccan isolates in order to use them as molecular markers for early and rapid detection of multidrug resistant strains. For that, all M. tuberculosis isolates received during 2002-2003 in the National Reference Laboratory of Tuberculosis in Morocco were subject to drug susceptibility tests for rifampicin and isoniazid and to a PCR probe method to detect specific mutation. Sequencing was performed for all genotypic rifampicin resistant isolates and also for four genotypic isoniazid resistant isolates randomly selected. Out of 187 M. tuberculosis positive cultures, 46 (24.6%) were phenotypically resistant to both rifampicin and isoniazid. Nucleotide mutations in rpoB531, rpoB526, rpoB516, katG315 and inh-15 codons associated with resistance to rifampin (RIF) and isoniazid (INH) were found respectively in 37/46 (80.4%) and 43/46 (93.5%) isolates. Genotypic multi drug resistance was then confirmed in 74% (34/46) isolates. The mutations at codon 315 of katG gene and at codons 531, 526 and 516 of rpoB gene are frequently found in MDR isolates which confirm their strong implication in the development of multidrug resistant tuberculosis. We concluded that these mutations are useful as molecular markers for detection of multidrug resistant isolates but are not yet sufficient to fully predict M. tuberculosis multidrug resistance.

show abstract

Section: Resultsmentioning

confidence: 99%

Molecular analysis of multidrug resistantMycobacterium tuberculosis isolates from Morocco

Sabouni¹,

Kourout

Chaoui

et al. 2008

Ann. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Only 33.4 % of mechanisms of resistance to INH have been known. Other researchers have been reported that mutations in promoters of ahpC and kasA gene are important within 12-24 and 10-14 %, respectively [14][15][16]18]. Therefore, these unknown cases of resistance to INH could be detected with assessment of ahpC and kasA gene promoters.…”

Section: Methods Sensitivity For Identification Of Inh-resistant Mtbmentioning

confidence: 96%

“…In addition, mutations in the regulatory region of the inhA gene have been reported in up to 32 % of INH-resistant isolates [14][15][16][17]. Mutations in other genomic regions, such as the promoter of ahpC gene and the kasA gene, have been reported in 12-24 and 10-14 % of INHresistant strains, respectively [14][15][16]18].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of Point Mutation Detection in Mycobacterium tuberculosis with Isoniazid Resistance Using Real-Time PCR and TaqMan Probe Assay

Riahi

Derakhshan

Mosavat

et al. 2014

Appl Biochem Biotechnol

View full text Add to dashboard Cite

Rapid methods for diagnosis of Mycobacterium tuberculosis (Mtb) drug resistance and choosing appropriate antibiotic treatment are pivotal. Thirty isoniazid (INH)-resistant and 30 INHsusceptible Mtb isolates were evaluated using minimum inhibitory concentration (MIC) method followed by multiplex real-time PCR (RT-PCR). Amplification refractory mutation system (ARMS) for detection of mutation in 315 codon of katG gene and single-nucleotide polymorphism (SNP) for detection of mutation in −15 (C>T) in the regulatory zone of mabA-inhA were carried out using the TaqMan method. Primers and probe were used for IS6110 region of Mtb as an internal amplification control. The sensitivity and specificity of the RT-PCR TaqMan probe for detection of Mtb complex were 100 %. Detection of INH-resistant Mtb using the ARMS method for KatG had 69 % sensitivity and 100 % specificity. The sensitivity and specificity of SNP in mabA-inhA fragment for detection of INH-resistant Mtb were 53 and 100 %, respectively. Furthermore, considering both regions, the sensitivity of RT-PCR has increased to 75 %. This study revealed that the qPCR-TaqMan method can be used as a standard tool for diagnosis of Mtb. Moreover, ARMS and SNP RT-PCR TaqMan methods can be used as rapid screening methods for detection of INH-resistant Mtb.

show abstract

“…Therefore, further expansion must be made of the results of non-mutating strains as research for inhA mutations, point mutations in the aphC-oxyR intergenic region (Wilson and Collins, 1996;Piatek et al, 2000), or mutations in the kasA gene (Mdluli et al, 1998;Lee et al, 1999).…”

mentioning

confidence: 99%

Genotypic and phenotypic characteristics of tunisian isoniazid-resistant Mycobacterium tuberculosis strains

Soudani¹,

Hadjfredj²,

Zribi³

et al. 2011

J Microbiol.

View full text Add to dashboard Cite

Forty three isoniazid (INH)-resistant Mycobacterium tuberculosis isolates were characterized on the basis of the most common INH associated mutations, katG315 and mabA -15C→T, and phenotypic properties (i.e. MIC of INH, resistance associated pattern, and catalase activity). Typing for resistance mutations was performed by Multiplex Allele-Specific PCR and sequencing reaction. Mutations at either codon were detected in 67.5% of isolates: katG315 in 37.2, mabA -15C→T in 27.9 and both of them in 2.4%, respectively. katG sequencing showed a G insertion at codon 325 detected in 2 strains and leading to amino acid change T326D which has not been previously reported. Distribution of each mutation, among the investigated strains, showed that katG S315T was associated with multiple-drug profile, high-level INH resistance and loss or decreased catalase activity; whereas the mabA -15C→T was more prevalent in mono-INH resistant isolates, but it was not only associated with a low-level INH resistance. It seems that determination of catalase activity aids in the detection of isolates for which MICs are high and could, in conjunction with molecular methods, provide rapid detection of most clinical INH-resistant strains.

show abstract

Contribution of kasA Analysis to Detection of Isoniazid-Resistant Mycobacterium tuberculosis in Singapore

Cited by 114 publications

References 16 publications

Molecular analysis of multidrug resistantMycobacterium tuberculosis isolates from Morocco

Molecular analysis of multidrug resistantMycobacterium tuberculosis isolates from Morocco

Evaluation of Point Mutation Detection in Mycobacterium tuberculosis with Isoniazid Resistance Using Real-Time PCR and TaqMan Probe Assay

Genotypic and phenotypic characteristics of tunisian isoniazid-resistant Mycobacterium tuberculosis strains

Contact Info

Product

Resources

About