Contribution of Immunohistochemistry in the Diagnosis of Lymphomas

Abstract

Objective: Making the interest of using immunohistochemistry clear in addition to histological test in the diagnosis of lymphomas. Methods: This is a retrospective study from January 2011 to June 2013 involving 18 cases of lymphomas collected in the teaching hospitals of Treichville and Yopougon. Results: 1) 38.88% diagnosis of LMNH B large-cell in histology against 38.88% LDGCB with IHC. 2) 11.11% diagnosis of LMNH small cell in histology against 11.11% with IHC. 3) 5.55% diagnosis of T lymphoma in histology against 27.77% diagnosis of T lymphoma with IHC. 4) 5.55% diagnosis of follicular lymphoma in histology against 11.11% diagnosis of LF with IHC. 5) 11.11% diagnosis of Burkitt lymphoma in histology against 5.55% diagnosis of LB with IHC. 6) 5.55% diagnosis of medullary hypoplasia in histology against 5.55% diagnosis of Hodgkin's disease. Our results confirm the contribution of immunohistochemistry in the diagnosis of lymphomas in addition to histological test. This is striking, especially as there is 27.77% of T lymphoma with immunohistochemistry against only 5.55% with histology.

Automatic CD30 scoring method for whole slide images of primary cutaneous CD30+lymphoproliferative diseases

“…Clinical diagnosis usually requires differentiation between benign LPDs of the skin and haematopoietic malignancies 1. IHC is necessary for disease diagnosis and differentiation, especially in cases that are easy to confuse and difficult to distinguish 8 9. Efficient and accurate qualitative and quantitative evaluations of CD30 + cell proportions play vital roles in distinguishing between CD30 + LPD subtypes, making treatment plans and prognoses feasible for patients.…”

“…Immunohistochemistry (IHC) is the gold standard for the diagnosis of cutaneous lymphomas 7. Dermatologists quantitatively and qualitatively evaluate abnormal immunohistochemical cells through optical microscopy, which is clinically significant for diagnosing specific disease types and for the developmental stages of cutaneous lymphomas 8 9. However, this process is highly dependent on the expertise of dermatologists and is easily interfered with by staining and isomerism.…”