Contribution of lipid environment to NADPH oxidase activity: Influence of sterol

Souabni, Hager; Machillot, Paul; Baciou, Laura

doi:10.1016/j.biochi.2014.10.006

Cited by 15 publications

(22 citation statements)

References 62 publications

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“…It has been reported that disruption of lipid rafts resulted in the downregulation of NADPH oxidase subunits in breast cancer cells [27] and in a compromised superoxide production [28], while lipid rafts have also been shown to maintain NADPH oxidase in an inactive state [29]. We could also observe that sterolenriched membranes decrease the rate of superoxide anion produced by recombinant NADPH oxidase [30,31]. This might be explained by different types of lipid raft redox platforms as described in [32].…”

Section: Accepted Manuscriptmentioning

confidence: 45%

“…The separation of the endoplasmic reticulum (ER) and plasma membranes (PM) were performed onto a discontinuous sucrose gradient (20-60 % sucrose) as described in [30]. For further analysis, 6 the ER and PM fractions were pelleted at 30,000 x g and resuspended in 1 mM EDTA, 30% sucrose, 20 mM Tris/HCl pH 8.0 and stored at -20 °C.…”

Section: Methodsmentioning

confidence: 99%

“…The final concentrations of AA needed for maximal activation with ER and PM have been previously optimized and deduced from concentration-response curves that plot the enzyme activity vs the AA concentration [30]. No significant difference of the optimal AA concentration could be identified for ER and PM.…”

Section: Temperature Dependence Of the Nadph Oxidase Activity In Yeasmentioning

confidence: 99%

“…To build these plots, since the concentration of NADPH was 10 times larger than its K m [30], we considered that the reaction was zero-order with respect to NADPH. The rate constants at each temperature k cat , were therefore taken equal to the initial rates of formation of O 2 …”

Section: Arrhenius Plots Of Nadph Oxidase Activitiesmentioning

confidence: 99%

“…The removal of ergosterol from P. pastoris plasma membranes was obtained as described in [30]. Briefly, the membrane fractions (6 mg of protein/ml) were incubated with 20 mM methyl-β-cyclodextrin (MβCD) at 25 °C in a roller shaker for 30 min.…”

Section: Ergosterol Extraction and Insertionmentioning

confidence: 99%

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The physicochemical properties of membranes correlate with the NADPH oxidase activity

Souabni

Wien

Bizouarn

et al. 2017

Biochimica et Biophysica Acta (BBA) - General Subjects

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High temperature promotes the rate of superoxide production. The key element of this enhancement is related to membrane properties such as thickness and viscosity and not to protein structural changes. Membrane viscosity that can be driven by sterols is a paramount parameter of Ea of NADPH oxidase activity. The membrane bilayer state modulated by its sterol content may be considered locally as an enzyme regulator. This article is part of a Special Issue entitled "Science for Life" Guest Editor: Dr. Austen Angell, Dr. Salvatore Magazù and Dr. Federica Migliardo.

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Section: Accepted Manuscriptmentioning

confidence: 45%

Section: Methodsmentioning

confidence: 99%

Section: Temperature Dependence Of the Nadph Oxidase Activity In Yeasmentioning

confidence: 99%

Section: Arrhenius Plots Of Nadph Oxidase Activitiesmentioning

confidence: 99%

Section: Ergosterol Extraction and Insertionmentioning

confidence: 99%

See 3 more Smart Citations

The physicochemical properties of membranes correlate with the NADPH oxidase activity

Souabni

Wien

Bizouarn

et al. 2017

Biochimica et Biophysica Acta (BBA) - General Subjects

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Alterations in the subcellular distribution of NADPH oxidase p47^phox in hypothalamic paraventricular neurons following slow‐pressor angiotensin II hypertension in female mice with accelerated ovarian failure

Kempen

Narayan

Waters

et al. 2015

J of Comparative Neurology

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At younger ages, women have a lower risk for hypertension than men, but this sexual dimorphism declines with the onset of menopause. These differences are paralleled in rodents following “slow-pressor” angiotensin II (AngII) administration: young male and aged female mice, but not young females, develop hypertension. There is also an established sexual dimorphism both in the cardiovascular response to the neurohypophyseal hormone arginine vasopressin (AVP) and in the expression of oxidative stress. We examined the relationship between AngII-mediated hypertension and the cellular distribution of the superoxide generating NADPH oxidase (NOX) in AVP-expressing hypothalamic paraventricular nucleus (PVN) neurons in “menopausal” female mice. Dual labeling immunoelectron microscopy was used to determine if the subcellular distribution of the organizer/adapter NOX p47phox subunit is altered in PVN dendrites following AngII administered (14 days) during the “postmenopausal” stage of accelerated ovarian failure (AOF) in young female mice treated with 4-vinylcyclohexene diepoxide. Slow-pressor AngII elevated blood pressure in AOF females and induced a significant a significant increase in near plasmalemmal p47phox and a decrease in cytoplasmic p47phox in PVN AVP dendrites. These changes are opposite to those observed in AngII-induced hypertensive male mice (Coleman et al., J. Neuroscience 33: 4308-16, 2013), and may be ascribed in part to baseline differences between young females and males in the near plasmalemmal p47phox on AVP dendrites seen in the present study. These findings highlight fundamental differences in the neural substrates of oxidative stress in the PVN associated with AngII hypertension in postmenopausal females compared with males.

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Mechanism and kinetics of the oxidative damage to ergosterol induced by peroxyl radicals in lipid media: a theoretical quantum chemistry study

Medina

Iuga

Trigos

2015

J of Physical Organic Chem

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In this work, we have studied the mechanisms and kinetics of the initial damage to ergosterol induced by• OOH and• OOCH 3 peroxyl radicals in a lipid media, using quantum chemistry and computational kinetics methods. The initial damage to ergosterol induced by these radicals occurs predominantly through the hydrogen transfer mechanism (HT) from the allylic position C14 of ergosterol. For the reaction of ergosterol with• OOH, the HT-9 pathway represents~90.8% of the overall rate constant, while in the case of• OOCH 3 , the HT-14 pathway represents more than~97.2% of the overall rate constant. The calculated overall reaction rates for the initial damage to ergosterol caused by the• OOH and • OOCH 3 are 2.05 × 10 6 and 6.26 × 10 4 M À1 s À1 , respectively, indicating that the oxidative damage to ergosterol initiated by these radicals, and probably other alkyl-peroxyl radicals, could be significantly dangerous to their integrity. Taking into account the calculated values of the overall rate coefficients, we can conclude that ergosterol is more susceptible to damage produced by peroxyl radicals than cholesterol and fatty acids. This suggests that fungal cells might be more sensitive to these radicals than animal cells, coinciding with the fact that one of the targets in combating fungi is precisely ergosterol. Finally, theoretical calculations confirm the antioxidant potential of ergosterol and could help explaining the nutraceutical activity of edible and medicinal mushrooms.

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Contribution of lipid environment to NADPH oxidase activity: Influence of sterol

Cited by 15 publications

References 62 publications

The physicochemical properties of membranes correlate with the NADPH oxidase activity

The physicochemical properties of membranes correlate with the NADPH oxidase activity

Alterations in the subcellular distribution of NADPH oxidase p47^phox in hypothalamic paraventricular neurons following slow‐pressor angiotensin II hypertension in female mice with accelerated ovarian failure

Mechanism and kinetics of the oxidative damage to ergosterol induced by peroxyl radicals in lipid media: a theoretical quantum chemistry study

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Contribution of lipid environment to NADPH oxidase activity: Influence of sterol

Cited by 15 publications

References 62 publications

The physicochemical properties of membranes correlate with the NADPH oxidase activity

The physicochemical properties of membranes correlate with the NADPH oxidase activity

Alterations in the subcellular distribution of NADPH oxidase p47phox in hypothalamic paraventricular neurons following slow‐pressor angiotensin II hypertension in female mice with accelerated ovarian failure

Mechanism and kinetics of the oxidative damage to ergosterol induced by peroxyl radicals in lipid media: a theoretical quantum chemistry study

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Alterations in the subcellular distribution of NADPH oxidase p47^phox in hypothalamic paraventricular neurons following slow‐pressor angiotensin II hypertension in female mice with accelerated ovarian failure