Contribution of Lysinibacillus sphaericus hemolysin and chitin-binding protein in entomopathogenic activity against insecticide resistant Aedes aegypti

Rojas-Pinzón, Paula Andrea; Dussán, Jenny

doi:10.1007/s11274-017-2348-9

Cited by 12 publications

(8 citation statements)

References 39 publications

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“…aegypti , which increased susceptibility to infection by DENV-2 [ 28 ]; Lysinibacillus , whose species Lysinibacillus sphaericus produces a toxin used as a larvicide in the control of Culex and Anopheles spp. [ 44 ]; Serratia , which is described as having anti- Plasmodium activity by stimulating the activation of the Toll receptor pathway in An. stephensi [ 45 ] and in Ae.…”

Section: Resultsmentioning

confidence: 99%

The influence of culture-dependent native microbiota in Zika virus infection in Aedes aegypti

et al. 2022

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Background Emerging and re-emerging vector-borne diseases (VBDs) pose a recurring threat to tropical countries, mainly due to the abundance and distribution of the Aedes aegypti mosquito, which is a vector of the Zika, dengue, chikungunya, and yellow fever arboviruses. Methods Female 3–5 day-old Ae. aegypti were distributed into two experimental groups: group I—survey of cultivable bacteria; sucrose group: fed only on sucrose, i.e., non-blood-fed (UF); blood-fed group: (i) fed with non-infected blood (BF); (ii) fed with blood infected with the Zika virus (BZIKV); (iii) pretreated with penicillin/streptomycin (pen/strep), and fed with non-infected blood (TBF); (iv) pretreated with pen/strep and fed blood infected with ZIKV, i.e., gravid with developed ovaries, (TGZIKV); group II—experimental co-infections: bacteria genera isolated from the group fed on sucrose, i.e., non-blood-fed (UF). Results Using the cultivable method and the same mosquito colony and ZIKV strain described by in a previous work, our results reveled 11 isolates (Acinetobacter, Aeromonas, Cedecea, Cellulosimicrobium, Elizabethkingia, Enterobacter, Lysinibacillus, Pantoea, Pseudomonas, Serratia, and Staphylococcus). Enterobacter was present in all evaluated groups (i.e., UF, BF, BZIKV, TBF, and TGZIKV), whereas Elizabethkingia was present in the UF, BZIKV, and TBF groups. Pseudomonas was present in the BZIKV and TBF groups, whereas Staphylococcus was present in the TBF and TGZIKV groups. The only genera of bacteria that were found to be present in only one group were Aeromonas, Lysinibacillus, and Serratia (UF); Cedacea, Pantoea and Acinetobacter (BF); and Cellulosimicrobium (BZIKV). The mosquitoes co-infected with ZIKV plus the isolates group fed on sucrose (UF) showed interference in the outcome of infection. Conclusions We demonstrate that the distinct feeding aspects assessed herein influence the composition of bacterial diversity. In the co-infection, among ZIKV, Ae. aegypti and the bacterial isolates, the ZIKV/Lysinibacillus–Ae. aegypti had the lowest number of viral copies in the head-SG, which means that it negatively affects vector competence. However, when the saliva was analyzed after forced feeding, no virus was detected in the mosquito groups ZIKV/Lysinibacillus–Lu. longipalpis and Ae. aegypti; the combination of ZIKV/Serratia may interfere in salivation. This indicates that the combinations do not produce viable viruses and may have great potential as a method of biological control. Graphical Abstract

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Section: Resultsmentioning

confidence: 99%

The influence of culture-dependent native microbiota in Zika virus infection in Aedes aegypti

et al. 2022

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“…Although mosquitoes lack acquired immunity, they display a series of immune responses against possible pathogens (Wang, Chang, Wang, Zheng, & Zou, ). Recently Rojas‐Pinzón and Dussán () proposed a model of what might be happening inside the larvae once L. sphaericus was ingested, suggesting that, at 24 hr, the larvae recognizes the bacterium as a potential pathogen and, as such, displays its cellular immune response (Rojas‐Pinzón & Dussán, ). However, by then, the bacterial load is high and the larva has already been weakened enough for it to die.…”

Section: Discussionmentioning

confidence: 99%

“…Based on the outcomes of previous studies where two mixed L. sphaericus strains resulted to be highly toxigenic against A. aegypti larvae (Rojas‐Pinzon & Dussán, ; Rojas‐Pinzón & Dussán, ; Rojas‐Pinzón, Silva‐Fernández, & Dussán, ), we evaluated the susceptibility of three A. aegypti populations from endemic regions to these two strains: L. sphaericus III(3)7 and L. sphaericus 2362. The strain III(3)7 was originally isolated from soil samples in an oak forest near Bogotá D.C., Colombia, and belongs to the CIMIC culture collection.…”

Section: Methodsmentioning

confidence: 99%

“…Despite studies describing the presence of mosquitocidal toxins (Mtx) in L. sphaericus vegetative cells (Liu, Porter, Wee, & Thanabalu, ; Thanabalu & Porter, ), it is only recently that the use of vegetative cells to control A. aegypti larvae has been discovered (Rojas‐Pinzon & Dussán, ; Santana‐Martínez, Silva, & Dussán, ). Along with the Mtx proteins, additional proteins that play an important role in toxicity were discovered (Gómez‐Garzón, Hernández‐Santana, & Dussán, ; Rojas‐Pinzón & Dussán, ), demonstrating, the biocontrol potential of L. sphaericus against A. aegypti larvae. In addition, the specificity of L. sphaericus against culicids (Berry et al, ; Charles, ) and its ability to persist and recycle in polluted environments are key factors that promotes its use against mosquito vectors (Berry, ).…”

Section: Introductionmentioning

confidence: 99%

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Susceptibility of three Colombian Aedes aegypti Linnaeus (Diptera: Culicidae) populations to a Lysinibacillus sphaericus formulation

Santana-Martínez

Rojas-Pinzón

Dussán

2019

J Applied Entomology

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The increased incidence of vector‐borne diseases and insecticide resistance in mosquitos constitute public health concerns in the tropics. Biological control is an effective alternative in the management of Aedes aegypti Linnaeus populations. Lysinibacillus sphaericus a bacterium proved to be harmless for non‐target organisms has shown promising entomopathogenic activity. In Colombia, the control of A. aegypti using L. sphaericus has not been contemplated as part of vector control programmes. To assess the susceptibility of three A. aegypti populations to a L. sphaericus formulation consisting of vegetative cells of 2362 and III(3)7 strains, simulated‐field bioassays were conducted in the municipalities of Ricaurte in Cundinamarca and Tauramena in Casanare, as well as in the municipal inspection of San Joaquin, Cundinamarca. Sixty larvae were deposited in a test device, which contained nine litres of chlorine‐free tap water and 100 ml of the bacterial formulation (109 CFU/ml). Six replicates were set‐up for treatment with bacteria and for the control. Larvae mortality was recorded at 24 and 48 hr. The three A. aegypti populations were susceptible to L. sphaericus formulation with a mortality rate higher than 90% at 48 hr. The formulation of L. sphaericus comprised of vegetative cells of 2362 and III(3)7 strains showed promising results that could guarantee the implementation of this formulation to control of A. aegypti populations.

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“…Aktivitas entomopatogen dari L. sphaericus disebabkan oleh keberadaan toxin yaitu Mosquitocidal toxin (Mtx protein), Binary toxin (BinA-BinB), Crystal toxin (Cry48/Cry49), S-layer protein, dan Hemolysin (Allievi et al, 2014;Jones et al, 2007;Lozano et al, 2011;Rojas-Pinzón & Dussán, 2017). Namun, bakteri ini memiliki variasi tingkat toksisitas dan tidak semua strain L. sphaericus memiliki protein toksin tersebut.…”

Section: Pendahuluanunclassified

Draft Genome of Lysinibacillus sphaericus Isolate 229C Pathogenic to Vector Mosquitoes

Viersanova

Purwanto

2021

JBT

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Lysinibacillus sphaericus is widely known as a bioinsecticide agent because it shows entomopathogenic activity against vector mosquitoes, especially of Culex and Anopheles spp. This bacterium known to have variations in toxicity. Its binary toxins, which is known to have a high toxicity, has a very low genetic variation, so that resistance problems has been reported. Therefore, exploration continues to be carried out to find a new effective and potential toxin to deal with the resistance problems. This study aims to analyze the genome of isolate 229C L. sphaericus, to identify the species of isolate 229C based on the 16S rRNA gene, and to identify toxin characteristics of the 229C isolate based on the results of genome sequence analysis. The 229C isolate was previously obtained from soil sample in Indonesia and showed a high pathogenicity against C. quenquefasciatus. Molecular identification was carried out with the 16S rRNA gene analysis. While draft genome and toxin analysis performed by conducting whole genome sequencing using Illumina Hiseq 2000, 250 bp pair-end protocol. The sequenced data then analized using freely available bioinformatics tools.The results of the molecular identification showed that the closest related species of isolate 229C was L. sphaericus. The isolate 229C has a genome size of 4.65 Mbp and G+C content of 36.83%. Toxin analysis showed that this isolate did not contain Mosquitocidal toxin (Etx/Mtx), binary toxin (Bin protein), crystal toxin (Cry48/Cry49 protein), nor Sphaericolysin genes. However, there are s-layer protein and hemolysin genes that also known to be associated with the toxicity of L. sphaericus to mosquitoes and possibly, are the answer to the problem of resistance to binary toxins. This result opens the opportunity for an analysis of the effectiveness of S-layer protein and Hemolysin against resistance population mosquitoes.

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Contribution of Lysinibacillus sphaericus hemolysin and chitin-binding protein in entomopathogenic activity against insecticide resistant Aedes aegypti

Cited by 12 publications

References 39 publications

The influence of culture-dependent native microbiota in Zika virus infection in Aedes aegypti

The influence of culture-dependent native microbiota in Zika virus infection in Aedes aegypti

Susceptibility of three Colombian Aedes aegypti Linnaeus (Diptera: Culicidae) populations to a Lysinibacillus sphaericus formulation

Draft Genome of Lysinibacillus sphaericus Isolate 229C Pathogenic to Vector Mosquitoes

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