Contribution of M-opsin-based color vision to refractive development in mice

Ji, Shunmei; Mao, Xiuyu; Zhang, Yifan; Lin, Yong Y.; Dai, Jinhui

doi:10.1016/j.exer.2021.108669

Cited by 6 publications

(3 citation statements)

References 55 publications

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“…A custom-made eccentric infrared photorefractor 24 and a spectral-domain optical coherence tomography (SD-OCT) system (TEL210C1; Thorlabs Inc.) were used to do the measurements as reported. 25 Using the heated tip of the 3D printing pen, we could engage the junction point, leading to its disconnection and enabling us to remove the spectacle. Before SD-OCT measurement, the mice were anesthetized with pentobarbital solution (70 mg/kg i.p.).…”

Section: Methodsmentioning

confidence: 99%

“…As described previously, 25 total protein extracts from retinas were prepared in RIPA lysis buffer (P0013B; Beyotime, China). Then, the BCA Protein Assay Kit (23235; ThermoFisher, USA) was used to measure the concentration.…”

Section: Methodsmentioning

confidence: 99%

“…As previously reported, 25 total RNA was extracted and reverse-transcribed into cDNA. The SYBR Green quantitative real-time PCR (qRT-PCR) kit (AG11701; Accurate Biotechnology [Hunan] Co., Ltd, China) and QuantStudio 5 real-tme PCR system (ThermoFisher, USA) were used to quantify the PCR-amplification products.…”

Section: Methodsmentioning

confidence: 99%

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Integrative Transcriptome and Proteome Analyses Elucidate the Mechanism of Lens-Induced Myopia in Mice

Ji,

Ye,

Yuan

et al. 2023

Invest. Ophthalmol. Vis. Sci.

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Purpose The purpose of this study was to investigate the underlying molecular mechanism of lens-induced myopia (LIM) through transcriptome and proteome analyses with a modified mouse myopia model. Methods Four-week-old C57BL/6J mice were treated with a homemade newly designed –25 diopter (D) lens mounting by a 3D printing pen before right eyes for 4 weeks. Refraction (RE) and axial dimensions were measured every 2 weeks. Retinas were analyzed by RNA-sequencing and data-independent acquisition liquid chromatography tandem mass spectrometry. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, and STRING databases were used to identify significantly affected pathways in transcriptomic and proteomic data sets. Western blot was used to detect the expression of specific proteins. Results The modified model was accessible and efficient. Mice displayed a significant myopic shift (approximately 8 D) following 4 weeks’ of lens treatment. Through transcriptomics and proteomics analysis, we elucidated 175 differently expressed genes (DEGs) and 646 differentially expressed proteins (DEPs) between binoculus. The transcriptomic and proteomic data showed a low correlation. Going over the mRNA protein matches, insulin like growth factor 2 mRNA binding protein 1 (Igf2bp1) was found to be a convincing biomarker of LIM, which was confirmed by Western blot. RNA-seq and proteome profiling confirmed that these two “omics” data sets complemented one another in KEGG pathways annovation. Among these, metabolic and human diseases pathways were considered to be correlated with the LIM forming process. Conclusions The newly constructed LIM model provides a useful tool for future myopia research. Combining transcriptomic and proteomic analysis may potentially brighten the prospects of novel therapeutic targets for patients with myopia.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%