Contribution of molecular microbiology to the study in water pollution removal of microbial community dynamics

Dabert, Patrick; Delgenès, Jean-Philippe; Moletta, R.; Godon, Jean‐Jacques

doi:10.1023/a:1015127411408

Cited by 32 publications

(17 citation statements)

References 89 publications

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“…Due to these restrictions of all cultivation based techniques a variety of cultivation independent methods has been developed to study the ecology of PAO in activated sludge (see reviews by DABERT et al, 2002;SEVIOUR et al, 2003) and more recently in natural aquatic ecosystems.…”

Section: Cultivation Techniquesmentioning

confidence: 99%

Methods for Detection and Quantification of Polyphosphate and Polyphosphate Accumulating Microorganisms in Aquatic Sediments

Hupfer¹,

Glöss

Schmieder³

et al. 2008

International Review of Hydrobiology

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It has been speculated that the microbial P pool is highly variable in the uppermost layer of various aquatic sediments, especially when an excessive P accumulation in form of polyphosphate (Poly-P) occurs. Poly-P storage is a universal feature of many different organisms and has been technically optimised in wastewater treatment plants (WWTP) with enhanced biological phosphorus removal (EBPR). In the recent past, new insights into mechanisms of P elimination in WWTP almost exclusively depended on the development and application of novel methods like 31 P-NMR spectroscopy and molecular methods for identifying Poly-P accumulating microorganisms (PAO). The aim of the present review is to compile current methods potentially available for detection and quantification of Poly-P in sediments and to complement it with yet unpublished results to validate their application in natural sediments. The most powerful tool for reliable Poly-P quantification in sediments is the liquid 31 P-NMR technique which has been successfully used for Poly-P measurements in a variety of aquatic sediments. But the microorganisms as well as mechanisms involved in Poly-P storage and cycling are largely unknown. Therefore, we also intend to stimulate future studies focusing on these encouraging topics in sediment research via the implementation of novel methods.

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Section: Cultivation Techniquesmentioning

confidence: 99%

Methods for Detection and Quantification of Polyphosphate and Polyphosphate Accumulating Microorganisms in Aquatic Sediments

Hupfer¹,

Glöss

Schmieder³

et al. 2008

International Review of Hydrobiology

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“…Numerous classical microbiological and molecular methods are available for the identification of methanogens (8), but most do not allow in situ analysis of a system. Fluorescence in situ hybridization (FISH) has been successfully used as a simple and rapid technique suitable for the assessment of a wide range of samples from environmental and engineered systems (10).…”

mentioning

confidence: 99%

Influence of Environmental Conditions on Methanogenic Compositions in Anaerobic Biogas Reactors

Karakashev

Batstone

Angelidaki

2005

Appl Environ Microbiol

455

246

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The influence of environmental parameters on the diversity of methanogenic communities in 15 full-scale biogas plants operating under different conditions with either manure or sludge as feedstock was studied. Fluorescence in situ hybridization was used to identify dominant methanogenic members of the Archaea in the reactor samples; enriched and pure cultures were used to support the in situ identification. Dominance could be identified by a positive response by more than 90% of the total members of the Archaea to a specific groupor order-level probe. There was a clear dichotomy between the manure digesters and the sludge digesters. The manure digesters contained high levels of ammonia and of volatile fatty acids (VFA) and were dominated by members of the Methanosarcinaceae, while the sludge digesters contained low levels of ammonia and of VFA and were dominated by members of the Methanosaetaceae. The methanogenic diversity was greater in reactors operating under mesophilic temperatures. The impact of the original inoculum used for the reactor start-up was also investigated by assessment of the present population in the reactor. The inoculum population appeared to have no influence on the eventual population.Anaerobic digestion is a simple and effective biological process for the treatment of different organic wastes and the production of energy in the form of biogas (1). A number of full-scale anaerobic digesters for biogas production have been developed and installed in Denmark during the last 20 years (27). They have been designed mainly for the codigestion of manure with a smaller fraction of other waste as a supplemental substrate. Specific environmental and operating factors influence anaerobic conversion processes in these codigestion plants, and similar factors also have an influence on wastewater primary and activated sludge (WW sludge) digesters. Some of the more important factors are temperature (4), ammonia level (7), and loading rate, which affects overall process stability, generally as measured by the concentrations of volatile fatty acids (VFA) in the digester (2).Anaerobic digestion is a multistep microbial process mediated by functionally different microbial groups-saccharide and amino acid fermenters, VFA oxidizers, and methanogens (17, 21). The two functional groups of methanogens (hydrogenotrophic and aceticlastic) have been well described in terms of physiology and phylogeny (11). Hydrogenotrophic methanogenesis and aceticlastic methanogenesis are also the key processes within anaerobic digestion, as when these processes are inhibited, digestion is effectively blocked at acidogenesis. Optimization of methanogenesis is difficult, because of both low growth rates and the susceptibility of the organisms to toxins (6).Organisms described as mediating hydrogenotrophic and aceticlastic methanogenesis are found within five phylogenetic orders (12). One of the hydrogenotrophic orders, Methanopyrales, has only hyperthermophilic member species and will not be considered further. The main orders and t...

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“…Although many microorganisms can degrade azo dyes [40][41][42][43][44][45][46][47] relatively few microbial species and strains have emerged as candidates for use in bioaugmentation [22,41,[48][49][50][51]. Before individual isolates can be recommended, comprehensive research is required to understand the role of individual microorganisms and their interactions with other microflora [30,52,53]. In this paper, various types of azo dye degrading microorganisms and their potential for bioaugmentation are discussed.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Azo Dyes by Three Isolated Bacterial Strains: An Environmental Bioremedial Approach

Shah¹

2011

JMBT

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Contribution of molecular microbiology to the study in water pollution removal of microbial community dynamics

Cited by 32 publications

References 89 publications

Methods for Detection and Quantification of Polyphosphate and Polyphosphate Accumulating Microorganisms in Aquatic Sediments

Methods for Detection and Quantification of Polyphosphate and Polyphosphate Accumulating Microorganisms in Aquatic Sediments

Influence of Environmental Conditions on Methanogenic Compositions in Anaerobic Biogas Reactors

Biodegradation of Azo Dyes by Three Isolated Bacterial Strains: An Environmental Bioremedial Approach

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