Contribution of native phosphorous-solubilizing bacteria of acid soils on phosphorous acquisition in peanut (Arachis hypogaea L.)

Abstract

The present investigation analyzes the in vitro P solubilization [Ca-P, Al-P, Fe(II)-P, and Fe(III)-P] efficiency of native PSB strains from acid soils of Odisha and exploitation of the same through biofertilization in peanut (Arachis hypogaea L.) growth and P acquisition. One hundred six numbers of soil samples with pH ≤ 5.50 were collected from five districts of Odisha viz., Balasore, Cuttack, Khordha, Keonjhar, and Mayurbhanj. One bacterial isolate from each district were selected and analyzed for their P solubilization efficiency in National Botanical Research Institute Phosphate broths with Ca, Al, and Fe-complexed phosphates. CTC12 and KHD08 transformed more amount of soluble P from Ca-P (CTC12 393.30 mg/L; KHD08 465.25 mg/L), Al-P (CTC12 40.00 mg/L; KHD08 34.50 mg/L), Fe(III)-P (CTC12 175.50 mg/L; KHD08 168.75 mg/L), and Fe(II)-P (CTC12 47.40 mg/L; KHD08 42.00 mg/L) after 8 days of incubation. The bioconversion of P by all the five strains in the broth medium followed the order Ca-P > Fe(III)-P > Fe(II)-P > Al-P. The identified five strains were Bacillus cereus BLS18 (KT582541), Bacillus amyloliquefaciens CTC12 (KT633845), Burkholderia cepacia KHD08 (KT717633), B. cepacia KJR03 (KT717634), and B. cepacia K1 (KM030037) and further studied for biofertilization effects on peanut. CTC12 and KHD08 enhanced the soil available P around 65 and 58% and reduced the amount of each Al about 79 and 81%, respectively, over the uninoculated control pots in the peanut rhizosphere. Moreover, all tested PSB strains could be able to successfully mobilize P from inorganic P fractions (non-occluded Al-P and Fe-P). The strains CTC12 and KHD08 increased the pod yield (114 and 113%), shoot P (92 and 94%), and kernel P (100 and 101%), respectively, over the control. However, B. amyloliquefaciens CTC12 and B. cepacia KHD08 proved to be the potent P solubilizers in promoting peanut growth and yield.

Effect of Phosphorus Solubilizers on Enzymatic Activity and Microbial Parameters in the Soil

“…It is also evident from the study that microorganisms compete with the plant roots for the orthophosphate, and accumulate the phosphorus making it temporarily unavailable to crops. It can be also explained that the inoculation of P solubilisers increase the microbial biomass phosphate which might be a reason for the reduction in MBC/P ratio in soils treated with P solubilisers as observed by Pradhan (2017). The variation observed in the MBC/P ratio may be attributed to the variation in microbial C and P in the respective treatments.…”

“…These microbes in turn can transform a small fraction of available P to MBP. This might be the reason for increase in MBP in P solubiliser treated plots (Pradhan et al, 2017). PSB was found to be superior for soil MBP and microbial activity at optimum P levels (Raghuveer et al, 2017).…”

Interactive Effects of Plant Growth-Promoting Rhizobacteria and Phosphates Sources on Growth and Phosphorus Nutrition of Soybean under Moderate Drought

“…phaseoli caused a significant improvement of seed yields and a concomitant increase uptake of macronutrients and micronutrients by common bean (Elkoca et al, 2010). From a practical consideration, the increase of fertilizer effectiveness with the inoculation of beneficial microorganisms may help to reduce the doses of P fertilizers and to increase fertilizer efficiency (Pradhan et al, 2017). In particular, P solubilizing bacteria are reported to play a significant role in increasing the P efficiency of both native and applied P and improving the growth and yield of various crops (Khan et al, 2009).…”

Influence and Role of Fungi, Bacteria, and Mixed Microbial Populations on Phosphorus Acquisition in Plants

“…Under low-phosphorus conditions, it affected the length, surface area, diameter and intracellular phosphatase activity of wheat roots, effects that are significantly correlated with better phosphorus absorption [155]. PSB can effectively increase the content of available phosphorus in soil, and promote the yield of peanut grain and the biomass of the bud [156]. PSB can provide plants with the required nutrients even at phosphorus levels ranging from 0.1 to 10 µm [157].…”