2015
DOI: 10.1080/21505594.2015.1049806
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Contribution of nuclease to the pathogenesis of Aeromonas hydrophila

Abstract: These authors contributed equally to this work. Keywords: Aeromonas hydrophila, nuclease, pathogenesisAeromonas hydrophila is a gram-negative bacterium that is widely distributed in aquatic environments and can cause septicemia in both fish and humans. However, the underlying mechanisms leading to severe infection are not well understood. In this study, an A. hydrophila nuclease (ahn) deletion mutant was constructed to investigate its contribution to pathogenesis. This mutant did not differ from the wild-type … Show more

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Cited by 29 publications
(20 citation statements)
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“…Primers and plasmids used in this experiment are listed in Tables 1 and 2. The procedures for mutant construction were described previously [22]. The Primers P1/P2 and P3/P4 were used in amplifications of upstream or downstream flanking fragments of crp via PCR respectively.…”
Section: Construction and Characterization Of Crp Mutantsmentioning
confidence: 99%
See 1 more Smart Citation
“…Primers and plasmids used in this experiment are listed in Tables 1 and 2. The procedures for mutant construction were described previously [22]. The Primers P1/P2 and P3/P4 were used in amplifications of upstream or downstream flanking fragments of crp via PCR respectively.…”
Section: Construction and Characterization Of Crp Mutantsmentioning
confidence: 99%
“…To determine the persistent carrier state of J118 and ∆crp strains in host, the competitive assay was performed as previously described [22]. J118 and ∆crp strains grown to mid-exponential phase in LB were harvested by centrifugation, washed and resuspended in PBS to a bacterial density of 5.0 × 10 6 CFU/mL, respectively.…”
Section: Resistance Against Host Clearance Of E Piscicidamentioning
confidence: 99%
“…Many proteins have proven to be important factors for the pathogenicity of A. hydrophila . In one study, a nuclease ( ahn ) deletion mutant was shown to attenuate the virulence of A. hydrophila in both fish and mice [27]. Jiang et al reported that the histone deacetylase is an important regulatory protein contributing to the pathogenicity of A. hydrophila [30].…”
Section: Discussionmentioning
confidence: 99%
“…The competitive assay was performed as described [27]. Overnight cultures of the WT and A.H.Δalr mutant were sub-cultured to OD 600 = 0.5.…”
Section: Methodsmentioning
confidence: 99%
“…Con relación a la A. hydrophila, esta es un bacilo Gram negativo que produce una variedad de enfermedades en algunas especies animales, desde acuáticos hasta terrestres, e incluso a los humanos (Ji et al, 2015); también, es causante de gastroenteritis e infecciones cutáneas y respiratorias (Zepeda-Velázquez, 2015), infecciones oculares, peritonitis, bacteriemia, meningitis, síndrome urémico hemolítico y fascitis necrotizante en humanos (Citterio;Biavasco, 2015); además, se considera un patógeno oportunista que provoca gran mortalidad en peces causando septicemia (Harikrishnan;Balasundaram, 2005).…”
Section: Introductionunclassified