Contribution of Podoviridae and Myoviridae bacteriophages to the effectiveness of anti-staphylococcal therapeutic cocktails

Kornienko, Maria; Kuptsov, Nikita; Gorodnichev, Roman; Bespiatykh, Dmitry; Guliaev, Andrei; Letarova, Maria A.; Kulikov, Eugene E.; Veselovsky, Vladimir A.; Malakhova, M V; Letarov, Andrey V.; Ilina, Elena N.; Shitikov, Egor

doi:10.1038/s41598-020-75637-x

Cited by 50 publications

(44 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The lytic bacteriophage vB_SauM-515A1 was isolated from the commercial Staphylococcus bacteriophage cocktail (batch P332) produced by the Microgen company (Moscow, Russia) using the Staphylococcus aureus strain SA515 (spa-type t008) as a host. General characterization of this phage and the conditions used for its cultivation were reported in our previous study [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

“…The one-step growth curve of vB_SauM-515A1 was measured as described previously, but the chloroform treatment was added to release the phage progeny from the infected cells prior to their natural lysis [ 26 ]. Briefly, cells of S. aureus SA515 at the early exponential phase (OD 600 = 0.12) were infected with vB_SauM-515A1 at an MOI of 0.01 and incubated at 37 °C with shaking (220 rpm).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptional Landscape of Staphylococcus aureus Kayvirus Bacteriophage vB_SauM-515A1

Kornienko

Fisunov

Bespiatykh

et al. 2020

Viruses

Self Cite

View full text Add to dashboard Cite

The Twort-like myoviruses (Kayvirus genus) of S. aureus are promising agents for bacteriophage therapy due to a broad host range and high killing activity against clinical isolates. This work improves the current understanding of the phage infection physiology by transcriptome analysis. The expression profiles of a typical member of the Kayvirus genus (vB_SauM-515A1) were obtained at three time-points post-infection using RNA sequencing. A total of 35 transcription units comprising 238 ORFs were established. The sequences for 58 early and 12 late promoters were identified in the phage genome. The early promoters represent the strong sigma-70 promoters consensus sequence and control the host-dependent expression of 26 transcription units (81% of genes). The late promoters exclusively controlled the expression of four transcription units, while the transcription of the other five units was directed by both types of promoters. The characteristic features of late promoters were long -10 box of TGTTATATTA consensus sequence and the absence of -35 boxes. The data obtained are also of general interest, demonstrating a strategy of the phage genome expression with a broad overlap of the early and late transcription phases without any middle transcription, which is unusual for the large phage genomes (>100 kbp).

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Transcriptional Landscape of Staphylococcus aureus Kayvirus Bacteriophage vB_SauM-515A1

Kornienko

Fisunov

Bespiatykh

et al. 2020

Viruses

Self Cite

View full text Add to dashboard Cite

show abstract

“…Based on genome length, low G+C content and gene organization, TSP phage is similar to that of wellstudied S. aureus lytic phages SLPW, VB_SauP_PhiAG01.3, P66, S13, and SCH1 [38][39][40]6] which were successfully applied for the treatment of S. aureus infections. Genes involved in structure, DNA replication, packaging and lysis showed best match with other Podoviridae phages listed in supplementary Table S3 [41].…”

Section: Discussionmentioning

confidence: 99%

TSP, a virulent Podovirus can control the growth of Staphylococcus aureus till 12 hours

Tabassum¹,

Alvi²,

Asif³

et al. 2021

Preprint

View full text Add to dashboard Cite

Methicillin-resistant Staphylococcus aureus (MRSA) is a prevailing nosocomial pathogen that causes a large number of diseases in healthcare and community settings. The MRSA causes infections in different tissues of immunocompromised individuals leading to increased morbidity and mortality. It possess various virulence mechanisms to show resistance against to a lot of beta-lactam antibiotics. To tackle this emerging issue of MRSA, there is an urgent need of antibiotic alternatives and utilizing lytic bacteriophages is one of the best promising therapeutic approach. In the present study, a lytic bacteriophage TSP was isolated from hospital wastewater against MRSA. Its morphology, physiology, host specificity, burst size and lytic spectrum were determined and complete genome sequence was analyzed. TSP phage efficiently inhibit bacterial growth for up to 12 hours. TSP phage showed broad lytic spectrum against clinical isolates of MRSA (78%) and MSSA (37%). It showed stability at varying temperatures (25ºC, 37ºC) and pH (5–9), while its maximum storage stability was observed at 4ºC. It had short latent period (20min) and high burst size (103 PFU/ infected cell). TSP genome sequence and restriction analysis revealed that its genome is linear having 17,987 bp in length with an average GC content of 29.7%. The TSP genome showed 98% similarity to S aureus phages SCH1, SCH11 and vB SauP-436A1. According to comparative genomic analysis and phylogenetic tree analysis, TSP phage can be considered as a member of genus “P68viruses”. The strong lytic activity, broad host range and short latent period along with absence of any lysogenic and toxic genes make TSP a very good candidate for phage therapy against MRSA infections if prove safe during in vivo studies.

show abstract

“…(e) It should be screened for toxin genes that can affect the patient. Myoviridae, Siphoviridae and Podoviridae families are used com-monly for phage therapy[49,50]. There are approximately 800 phages against pathogens such as Escherichia, Morganella, Klebsiella, Enterococcus, Pseudomonas, Staphylococcus and Salmonella[31].…”

mentioning

confidence: 99%

Bacteriophage Therapy of Bacterial Infections: The Rediscovered Frontier

2021

View full text Add to dashboard Cite

Antibiotic-resistant infections present a serious health concern worldwide. It is estimated that there are 2.8 million antibiotic-resistant infections and 35,000 deaths in the United States every year. Such microorganisms include Acinetobacter, Enterobacterioceae, Pseudomonas, Staphylococcus and Mycobacterium. Alternative treatment methods are, thus, necessary to treat such infections. Bacteriophages are viruses of bacteria. In a lytic infection, the newly formed phage particles lyse the bacterium and continue to infect other bacteria. In the early 20th century, d’Herelle, Bruynoghe and Maisin used bacterium-specific phages to treat bacterial infections. Bacteriophages are being identified, purified and developed as pharmaceutically acceptable macromolecular “drugs,” undergoing strict quality control. Phages can be applied topically or delivered by inhalation, orally or parenterally. Some of the major drug-resistant infections that are potential targets of pharmaceutically prepared phages are Pseudomonas aeruginosa, Mycobacterium tuberculosis and Acinetobacter baumannii.

show abstract

Contribution of Podoviridae and Myoviridae bacteriophages to the effectiveness of anti-staphylococcal therapeutic cocktails

Cited by 50 publications

References 43 publications

Transcriptional Landscape of Staphylococcus aureus Kayvirus Bacteriophage vB_SauM-515A1

Transcriptional Landscape of Staphylococcus aureus Kayvirus Bacteriophage vB_SauM-515A1

TSP, a virulent Podovirus can control the growth of Staphylococcus aureus till 12 hours

Bacteriophage Therapy of Bacterial Infections: The Rediscovered Frontier

Contact Info

Product

Resources

About