Contribution of progesterone, follicle stimulating hormone and glucocorticoids in survival of serum-free cultured granulosa cell explants

Mussche, Sylvie; D’Herde, Katharina

doi:10.1677/joe.0.1690321

Cited by 14 publications

(10 citation statements)

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“…Gonadotrophins can induce the expression of pro-survival molecules, including bcl-2, GATA-4, FLIP and XIAP, and decrease the expression of proapoptotic molecules, such Bax, Apaf1, Fas/FasL and p53 [112,132,134]. It has been shown that FSH can mediate both progesterone-dependent and progesterone-independent survival pathways in pre-ovulatory avian GCs [135].…”

Section: Gonadotropins and Intraovarian Regulatorsmentioning

confidence: 99%

Life and death of female gametes during oogenesis and folliculogenesis

et al. 2008

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The vertebrate ovary is an extremely dynamic organ in which excessive or defective follicles are rapidly and effectively eliminated early in ontogeny and thereafter continuously throughout reproductive life. More than 99% of follicles disappear, primarily due to apoptosis of granulosa cells, and only a minute fraction of the surviving follicles successfully complete the path to ovulation. The balance between signals for cell death and survival determines the destiny of the follicles. An abnormally high rate of cell death followed by atresia can negatively affect fertility and eventually lead irreversibly to premature ovarian failure. In this review we provide a short overview of the role of programmed cell death in prenatal differentiation of the primordial germ cells and in postnatal folliculogenesis. We also discuss the issue of neo-oogenesis. Next, we highlight molecules involved in regulation of granulosa cell apoptosis. We further discuss the potential use of scores for apoptosis in granulosa cells and characteristics of follicular fluid as prognostic markers for predicting the outcome of assisted reproduction. Potential therapeutic strategies for combating premature ovarian failure are also addressed.

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Section: Gonadotropins and Intraovarian Regulatorsmentioning

confidence: 99%

Life and death of female gametes during oogenesis and folliculogenesis

et al. 2008

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“…Progesterone treatment increases granulosa cell survival in quail (22), cow (23,24), rat (18,20,25), and primates (21), including humans (19), yet the mechanism of progesterone's antiapoptotic action remains unclear. Although this progestin action has been attributed to the presence of PR in some cell models (22)(23)(24)(25), it has also been demonstrated in cells that lack PR activity (18 -21).…”

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confidence: 99%

Progestin Signaling Through mPRα in Atlantic Croaker Granulosa/Theca Cell Cocultures and Its Involvement in Progestin Inhibition of Apoptosis

Dressing

Pang²,

Dong³

et al. 2010

Endocrinology

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Although there is substantial evidence that membrane progestin receptors (mPRs) perform a critical physiological role in meiotic maturation of fish oocytes, it is unknown whether they are also intermediaries in progestin signaling in the surrounding follicular cells. Here, we show that mPRα protein is located on the plasma membranes of both granulosa and theca cells (G/T cells) isolated from Atlantic croaker ovaries and is associated with the presence of a single high affinity, limited capacity, pertussis toxin-sensitive, specific progestin [17,20β,21-trihydroxy-4-pregnen-3-one (20β-S)] membrane binding site with the characteristics of mPRα. Treatment of G/T cells with 20β-S caused rapid G protein activation and a transient, pertussis toxin-sensitive, decrease in cAMP levels, whereas the selective nuclear progesterone receptor agonist, R5020, did not cause G protein activation, consistent with previous reports on mPRα signaling. 20β-S treatment decreased serum starvation-induced cell death in both G/T cells and in seatrout mPRα-transfected MDA-MB-231 cells, whereas R5020 was ineffective. Moreover, a selective mPRα agonist, 10-ethenyl-19-norprogesterone, mimicked the protective action of 20β-S against cell death, which was lost upon knockdown of mPRα protein but not after progesterone receptor knockdown, further demonstrating an involvement of mPRα. Signaling molecules involved in inhibition of apoptosis, Erk and serine-threonine kinase, were activated in G/T cells by 20β-S, which suggests a potential mechanism for mPRα inhibition of apoptosis. This is the first study to demonstrate endogenous mPR signaling in the ovarian follicle and to suggest a novel physiological role for mPRα in mediating the antiapoptotic actions of progestins in ovarian follicle cells.

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“…Overexpression of p53 results in granulosa cell apoptosis Kim et al, 1999;Hughes and Gorospe, 1991;Wang et al, 2002;Mussche and D'Herde, 2001;Orisaka et al, 2006;Zwain and Amato, 2001). …”

Section: Gonadotropinsmentioning

confidence: 99%

Role of apoptosis-related factors in follicular atresia

Gumus¹,

Kılıç²,

Zülfikaroğlu³

2013

Int. J. Genet. Mol. Biol.

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Follicular atresia is the term used for the fate of follicles which undergo degenerative changes before rupturing during ovulation. Recent studies suggest that granulosa cell apoptosis play a major role in follicular atresia. The factors which lead the cell to apoptosis and which protect the cell death, still remain complicated and more studies are needed to elucidate the whole process. Here in this review, we aimed to simplify the factors and mechanisms taking place in granulosa cell apoptosis, to make the process more understandable.Key words: Granulosa cell, apoptosis, follicular atresia. INTRODUCTİONWhen a female human embryo is 8-week-old, she has 600,000 germ cells in her gonads and this number increases to 6 to 7 million at 20 weeks of gestation. But with the increasing rate of atresia, the number declines progressively and 1 million oocytes are present in the newborn, whereas 300,000 oocytes remain in puberty, of which approximately 400 will ovulate during the fertile lifespan (Oktem and Oktay, 2008;Gougeon, 1996;Matova and Cooley, 2001).'Follicular atresia' term is used to define antral follicles undergoing degenerative changes before rupturing during ovulation. It is initiated within the granulosa cell layer and subsequently in the theca cells Hsueh et al., 1994). In mammals, the basic mechanism of follicular atresia is apoptosis (Depalo et al., 2003). Apoptosis is a way which multicellular organisms use to eliminate unwanted cells in response to developmental signals or toxic stimuli (Quirck et al., 2004). It is regulated at the level of transcription or translation (Manabe et al., 2008). Major morphological characteristics of apoptosis are the internucleosomal DNA fragmentation, cell shrinkage, plasma membrane blebbing, and the apoptotic body formation (Schwartzman and Cidlowski, 1993).Granulosa cells possess endogenous pathways to trigger apoptosis, that are inhibited in the presence of survi-*Corresponding author. E-mail: zebru33@gmail.com. Tel: +90 (312) 2158652. Fax: +90 (312) 3124931.val factors (Quirck et al., 2004). To date, many apoptosisrelated factors have been implicated in follicular atresia, including death ligands and receptors, Bcl-2 family proteins, Nodal, caspases, growth factors, gonadotropins, and calcium. In this report, we will overview these factors one by one. Proapoptotic regulators in the cell death receptorligand systemDeath receptors constitute a subgroup within the tumor necrosis factor (TNF) receptor family. They are located on the cell surface, anchored to the cell membrane, are trimerized and have cytoplasmic death domains (DDs) which are necessary to induce apoptosis (Park et al., 2005;Ashkenazi and Dixit, 1998;Wallach et al., 1999). Fas receptor, TNF receptor, and TNF related apoptosis inducing ligand receptors (TRAILr) are the members of TNF receptor family that are found to have roles in follicular atresia in mammalian ovaries (Park et al., 2005).In most of the cases, the cell death receptor-mediated apoptosis takes place as follows:1) The cell death ligand binds to...

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Contribution of progesterone, follicle stimulating hormone and glucocorticoids in survival of serum-free cultured granulosa cell explants

Cited by 14 publications

References 60 publications

Life and death of female gametes during oogenesis and folliculogenesis

Life and death of female gametes during oogenesis and folliculogenesis

Progestin Signaling Through mPRα in Atlantic Croaker Granulosa/Theca Cell Cocultures and Its Involvement in Progestin Inhibition of Apoptosis

Role of apoptosis-related factors in follicular atresia

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