Contribution of spermatozoal centrosomes to the microtubule-organizing centre in Antarctic minke whale (<i>Balaenoptera bonaerensis</i>)

Kobayashi, Toshihiro; Amemiya, Kazue; Takeuchi, Kana; Tsujioka, T.; Tominaga, Keiichiro; Hirabayashi, Masumi; Ishikawa, Hajime; Fukui, Yutaka; Hochi, Shinichi

doi:10.1017/s0967199406003522

Cited by 4 publications

(5 citation statements)

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“…To assess MTOC function in fertilized oocytes, FD-ICSI, control ICSI and IVF oocytes were subjected to immunostaining [32]. For FD-ICSI and control ICSI, injected oocytes were activated with ionomycin and ethanol at a 4-h interval and further cultured in TCM199+5% FBS for 3 h at 38.5 C under 5% CO2 in air.…”

Section: Mtoc Function In Freeze-dried Bull Spermmentioning

confidence: 99%

“…The length of the comet tail (pixel) was measured from the center of gravity of the head, and the DNA content in the comet tail was determined with the relative fluorescent intensity and defined as % DNA liberated. The DNA fragmentation index (Moment) was calculated as the length of the comet tail × the % DNA liberated.To assess MTOC function in fertilized oocytes, FD-ICSI, control ICSI and IVF oocytes were subjected to immunostaining [32]. For FD-ICSI and control ICSI, injected oocytes were activated with ionomycin and ethanol at a 4-h interval and further cultured in TCM199+5% FBS for 3 h at 38.5 C under 5% CO2 in air.…”

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confidence: 99%

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Procedure for Bovine ICSI, not Sperm Freeze-drying, Impairs the Function of the Microtubule-organizing Center

Hara

Abdalla

Morita

et al. 2011

Journal of Reproduction and Development

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Abstract. This study was designed to investigate whether freeze-dried (FD) bull spermatozoa maintained the function of the microtubule-organizing center (MTOC) after rehydration and intracytoplasmic sperm injection (ICSI). In a preliminary attempt, the cleavage and blastocyst formation rates in FD-ICSI zygotes (36 and 1%, respectively) were found to be considerably lower than those in control ICSI zygotes (67 and 21%, respectively) or in IVF zygotes (78 and 43%, respectively). An alkaline comet assay indicated that the DNA fragmentation index (length of comet tail × % DNA liberated) was not significantly different between fresh and FD spermatozoa. In the main experiment, formation of sperm-asters in the FD-ICSI oocytes 7 h postinsemination occurred at a similar rate when compared with the control ICSI oocytes (41 vs. 49%). Among the oocytes exhibiting sperm aster formation, the extent of microtubule network assembly was comparable between the FD-ICSI and control ICSI groups. However, the MTOC of the ICSI oocytes was not as functional as that of IVF oocytes in terms of the aster formation rate (97%) and the fluorescent intensity of the microtubule network (2.0 folds). These results suggest that the freeze-drying process per se had no adverse effect on maintaining the MTOC function in bull spermatozoa. Key words: Bovine Intracytoplasmic sperm injection (ICSI), Freeze-drying, Microtubule-organizing center (MTOC), Sperm-aster (J. Reprod. Dev. 57: [428][429][430][431][432] 2011) centrosome is composed of a pair of centrioles surrounded by pericentriolar materials such as γ-tubulin, centrin and pericentrin. Since an interphase network of microtubules and the mitotic bipolar spindle are nucleated from the centrosome, the centrosome is considered to be the microtubule-organizing center (MTOC) [1]. During fertilization in most mammalian species, the centrosome brought into an oocyte by a spermatozoon plays a critical role in assembly of the microtubule network that brings both male and female pronuclei to the center of the newly formed zygote, as reported in humans [2], rhesus monkeys [3], rabbits [4], pigs [5] and cattle [6]. Interestingly, paternal inheritance of the MTOC does not occur in the mouse [7] and rat [8], and the microtubule network developed from multiple cytoplasmic asters, instead of a single sperm-aster, is involved in the migration of pronuclei [9].Freeze-drying has been proposed as an alternative method to preserve mammalian spermatozoa [10], although freeze-dried (FD) spermatozoa after rehydration lose their motility and application of intracytoplasmic sperm injection (ICSI) technique is necessary. Rodent spermatozoa can be stored practically at refrigeration temperatures [11][12][13]. However, in large domestic species, in vitro production of blastocysts derived from FD-ICSI is still considered to be a challenging endeavor [14][15][16]. We have recently reported that the freeze-drying protocol slightly reduced the ability of bull spermatozoa to induce calcium oscillations [17] and that it had no a...

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Section: Mtoc Function In Freeze-dried Bull Spermmentioning

confidence: 99%

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confidence: 99%

Procedure for Bovine ICSI, not Sperm Freeze-drying, Impairs the Function of the Microtubule-organizing Center

Hara

Abdalla

Morita

et al. 2011

Journal of Reproduction and Development

Self Cite

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“…Assembly of the microtubule network is promoted by oocyte activation. The ratio of aster diameter to oocyte diameter is 0.57 in injected and activated oocytes (Kobayashi et al ., 2006).…”

Section: Interspecies Fertilization Of Whale Spermmentioning

confidence: 99%

Recent progress in reproduction of whale oocytes

Zheng

2011

Zygote

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Whale oocytes recovered from follicles can be matured in vitro. Whale sperm and mature oocytes can be used for in vitro fertilization (IVF), and IVF embryos have the ability to develop to morula stage. Whale sperm injected into bovine or mouse oocytes can activate the oocytes and form pronucleus. Interspecies somatic cell nuclear transfer embryos have been reconstructed with whale somatic cell nucleus and enucleated bovine or porcine oocytes, and interspecies cloned embryos can develop in vitro. This paper reviews recent progress in maturation, fertilization and development of whale oocytes.

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“…This seems very promising especially when bovine embryos have been already produced with ICSI using freeze-dried spermatozoa, heat-dried sperm heads or frozen-thawed oocytes (Keskintepe et al, 2002, Rho et al, 2004Lee & Niwa, 2006). Interspecies microfertilization using bovine oocytes can also be used to study key processes during sperm-oocyte fusion, oocyte activation and fertilization (Li et al, 2003;Kobayashi et al, 2006). This has a special relevance in human fertility, as bovine ICSI has been suggested to be an appropriate model to assess human sperm oocyte activation ability (Terada et al, 2004).…”

Section: In Vitro Assisted Fertilizationmentioning

confidence: 99%

<b>Assisted Reproductive Technologies in Cattle: Applications in Livestock Production, Biomedical Research and Conservation Biology</b>

Velazquez

2008

Annu Rev Biomed Sci

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Velazquez MA. Assisted Reproductive Technologies in Cattle: Applications in Livestock Production, Biomedical Research and Conservation Biology. ARBS Annu Rev Biomed Sci 2008;10:36-62. In cattle, assisted reproductive technologies (ART) can be defined as techniques that manipulate reproductive-related events and/or structures to achieve pregnancy with the final goal of producing healthy offspring in bovine females. The present review includes manipulation of female reproductive tract physiology, artificial insemination, multiple ovulation and embryo transfer, in vitro production of embryos, in vitro assisted fertilization, cloning, transgenesis, xenografting-germ cell transplantation, preimplantation genetic diagnosis and sperm sexing. This review shows that several ART are being currently applied commercially in the cattle industry with acceptable results. On the other hand, others have low efficiency in producing cattle offspring and are predominantly applied in experimental settings. Several of these ART can cause detrimental effects at the prenatal and postnatal period and therefore they need to be improved. However, even if these bovine-related biotechnologies are properly improved, they might be more useful in the conservation of endangered ungulates, production of pharmaceuticals, or as experimental models for human reproduction.

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Contribution of spermatozoal centrosomes to the microtubule-organizing centre in Antarctic minke whale (Balaenoptera bonaerensis)

Cited by 4 publications

References 38 publications

Procedure for Bovine ICSI, not Sperm Freeze-drying, Impairs the Function of the Microtubule-organizing Center

Procedure for Bovine ICSI, not Sperm Freeze-drying, Impairs the Function of the Microtubule-organizing Center

Recent progress in reproduction of whale oocytes

<b>Assisted Reproductive Technologies in Cattle: Applications in Livestock Production, Biomedical Research and Conservation Biology</b>

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