Contribution of spoligotyping and MIRU-VNTRs to characterize prevalent Mycobacterium tuberculosis genotypes infecting tuberculosis patients in Morocco

Molecular Typing of Mycobacterium Tuberculosis Complex by 24-Locus Based MIRU-VNTR Typing in Conjunction with Spoligotyping to Assess Genetic Diversity of Strains Circulating in Morocco

“…As summarized in Table 2 , 24-locus based MIRU-VNTR typing largely reduced the clustering defined by spoligotyping alone or even by spoligotyping combined with 12-locus MIRU-VNTR typing, from 76% and 48% to 14.6%, respectively. It is noteworthy that this effect was particularly noticed for large clusters initially defined by spoligotypes such as ST42 (LAM) and ST50 (Haarlerm), which are predominant in Morocco, as seen in this study as well in previous reports [ 16 , 17 ]. After analysis with 24 loci, these initial spoligotype-based clusters were reduced to a few clusters of at most three isolates.…”

“…This is the first study to explore the usefulness of standard 24-locus based MIRU-VNTR typing for molecular epidemiological study of MTBC strains from Morocco. Hitherto, MTBC population structure and tuberculosis transmission were only studied at the national level by using spoligotyping alone or in conjunction with 12-loci MIRUs [ 16 , 17 ]. In comparison to these two typing methods, the discriminatory power of 24-locus MIRU-VNTR typing has been shown to be higher in a number of other settings in Europe and elsewhere [ 4 , 34 , 35 ], and often similar to that of IS 6110 -based RFLP when comparison with the latter method were made [ 6 – 9 ].…”

“…Only a few studies have investigated MTBC genetic diversity in Morocco [ 12 – 17 ]. El Baghdadi et al .…”

“…(2012) and Chaoui et al . (2014) used spoligotyping alone or in combination with a 12-locus MIRU-VNTR typing for molecular epidemiological analyses of Moroccan isolates [ 13 , 15 – 17 ]. The purpose of the present study was therefore to assess standard 24-locus based MIRU-VNTR typing for the first time, on a panel of MTBC isolates collected from diverse geographical cities from Morocco.…”

Clonality and genetic profiles of drug‐resistant Mycobacterium tuberculosis in the Eastern Cape Province, South Africa

“…Several methods such as IS6110 restriction fragment length polymorphism (RFLP), spoligotyping, and mycobacterial interspersed repetitive-unit-variable numbers of tandem repeat (MIRU-VNTR), have been used for molecular typing of M. tuberculosis (Cavusoglu, Turhan, Akinci, & Soyler, 2006;Cerezo et al, 2012;Mokrousov et al, 2004;Sola et al, 2003). IS6110 RFLP typing is arduous, requires enormous quantities of DNA and has deprived discriminatory power on M. tuberculosis isolates with little or no IS61 l0 copy number (Chaoui, Zozio, & Lahlou, 2014), although it is known as the orientation technique for genotyping of M. tuberculosis strains (Mathema, Kurepina, Bifani, & Kreiswirth, 2006;van Soolingen, 2001). These limitations of IS6110 have been compensated by the development of PCR-based methods such as spoligotyping and MIRU-VNTR (Supply, Magdalena, Himpens, & Locht, 1997;Supply et al, 2006).…”