Contribution of Src and Ras pathways in FGF-2 induced endothelial cell differentiation

Klint, Peter; Kanda, Shigeru; Kloog, Yoel; Claesson‐Welsh, Lena

doi:10.1038/sj.onc.1202680

Cited by 112 publications

(90 citation statements)

References 46 publications

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“…Dependent on the cell model, however, abrogation of FGF-stimulated sustained MAP kinase activity fails to affect neuronal cell differentiation in vitro (53). Similar data exist for endothelial cell differentiation; treatment of endothelial cells with the specific MEK inhibitor PD98059 attenuates acute and sustained MAP kinase activity, but still allows tube formation of the cells (54). Instead, activation of the serine/threonine kinase Raf, which is positioned immediately downstream of Ras, and activation of the cytoplasmic tyrosine kinase Src, appears to be necessary for differentiation in at least certain neuronal and endothelial cell models (53,54).…”

Section: Fgf Receptor Signal Transduction In Biological Responsessupporting

confidence: 54%

“…Similar data exist for endothelial cell differentiation; treatment of endothelial cells with the specific MEK inhibitor PD98059 attenuates acute and sustained MAP kinase activity, but still allows tube formation of the cells (54). Instead, activation of the serine/threonine kinase Raf, which is positioned immediately downstream of Ras, and activation of the cytoplasmic tyrosine kinase Src, appears to be necessary for differentiation in at least certain neuronal and endothelial cell models (53,54). Axonal growth of neuronal cells has moreover been shown to be stimulated by cell adhesion, via cell adhesion molecule-mediated activation of FGF receptors (100,101).…”

Section: Fgf Receptor Signal Transduction In Biological Responsesmentioning

confidence: 56%

“…Moreover, Src is a substrate for focal adhesion kinase (52) and could thereby play a role in migratory events. Src kinase activity appears to be critical in differentiation of neuronal cells (53) and endothelial cells (54). Such data are based both on the ectopic expression of dominantnegative Src, and of the transforming variant v-Src, as well as on the use of an apparently specific Src kinase inhibitor, PP1 (55).…”

Section: Sh2-domain Protein Interactions With Fgf Receptorsmentioning

confidence: 99%

“…neurite outgrowth of the rat phaeochromocytoma cell line PC12 (56) and in proliferation, but not in differentiation, i.e. formation of vessel-like structures in three-dimensional collagen gels, of a murine brain endothelial cell line (54). FRS2 has been proposed to correspond to the classical major FGFR-1 substrate denoted p90.…”

Section: Sh2-domain Protein Interactions With Fgf Receptorsmentioning

confidence: 99%

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Signal transduction by fibroblast growth factor receptors

Claesson‐Welsh¹

1999

Front Biosci

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Section: Fgf Receptor Signal Transduction In Biological Responsessupporting

confidence: 54%

Section: Fgf Receptor Signal Transduction In Biological Responsesmentioning

confidence: 56%

Section: Sh2-domain Protein Interactions With Fgf Receptorsmentioning

confidence: 99%

Section: Sh2-domain Protein Interactions With Fgf Receptorsmentioning

confidence: 99%

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Signal transduction by fibroblast growth factor receptors

Claesson‐Welsh¹

1999

Front Biosci

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“…The balance between kinases and phosphatases (Berndt, 1999;Biethahn et al, 1999;Saxena and Mustelin, 2000;Ballif and Blenis, 2001) as well as other mechanisms (Keyse and Ginsburg, 1993) regulate the activation of the MAPK family members. Dysregulation of the Ras/Raf/MEK/ERK pathway plays a major role in cancer pathogenesis and has been associated with a multitude of pathological conditions, including oncogenic transformation and tumorigenesis (Klint et al, 1999;Sebolt-Leopold, 2000;Lee and McCubrey, 2002).…”

Section: Introductionmentioning

confidence: 99%

Dual-specificity phosphatase Pyst2-L is constitutively highly expressed in myeloid leukemia and other malignant cells

et al. 2003

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Northern blotting confirmed previous results indicating that the mitogen-activated protein kinase (MAPK) phosphatase Pyst2-L was highly expressed in leukocytes obtained from acute myeloid leukemia (AML) patients. High levels of Pyst2-L mRNA were expressed in bone marrow (BM) and peripheral leukocytes from nine AML and acute lymphoblastic leukemia (ALL) patients. BM from healthy individuals expressed very low levels of Pyst2-L. Whereas high levels of Pyst2-L mRNA and protein were detected in several leukemia cell lines, Pyst2-L mRNA was detected neither in 33/34 samples of normal peripheral blood mononuclear cells (PBMC) nor in leukocyte fractions enriched with CD34 þ cells. Certain solid tumor and lymphoblastoid cell lines expressed high levels of Pyst2-L mRNA. In view of the association of Pyst2-L to MAPK signaling cascades, we tested if cell activation, a process involving MAPK signaling, influences Pyst2-L expression. Indeed, activation of T cells and endothelial cells increased Pyst2-L in these cells. Furthermore, TPA, a known MAPK activator, induces the expression of both Pyst2-L mRNA as well as the Pyst2-L protein in leukemia cells. This induction was partially inhibited by PD098059, an Mek1/2-specific inhibitor. Based on the results of this and previous studies, we hypothesize that the high levels of Pyst2-L detected in the active state of AML and ALL diseases and in other types of cancer reflect an altered MAPK signaling pathway in such malignant processes. This alteration may be the result of a failed attempt to counter the constitutive activation of MAPK in transformed cells or alternatively, may represent the activated state of such cells.

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Green Fluorescent Protein in the Study of Neuronal Signaling Pathways

2001

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In recent years, techniques have been established for transiently co-transfecting cells with cDNA of the jellyfish green fluorescent protein (GFP), a reporter gene that encodes a non-toxic marker. This approach can be applied to primary neurons where it has become especially useful for the study of neuronal second messenger pathways. This unit describes procedures for transfecting neurons in primary culture: transfection with GFP DNA, including co-transfecting with separate GFP and gene-of-interest constructs, transfecting with a single construct containing the gene of interest fused to a GFP gene, and transfecting with a single construct containing separate gene-of-interest and GFP cassettes. Also included is a method for the rapid, large-scale preparation of a nearly homogeneous population of neurons from rat cerebellum. The Commentary provides several examples of how this approach can be applied to specific biological questions on neuronal signaling pathways.

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Contribution of Src and Ras pathways in FGF-2 induced endothelial cell differentiation

Cited by 112 publications

References 46 publications

Signal transduction by fibroblast growth factor receptors

Signal transduction by fibroblast growth factor receptors

Dual-specificity phosphatase Pyst2-L is constitutively highly expressed in myeloid leukemia and other malignant cells

Green Fluorescent Protein in the Study of Neuronal Signaling Pathways

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