Contribution of symbiotic microbiota to adaptation of Drosophila melanogaster to an unfavorable growth medium

Панченко, П. Л.; Kornilova, M. B.; Perfilieva, K. S.; Марков, А. В.

doi:10.1134/s1062359017040100

Cited by 9 publications

(16 citation statements)

References 39 publications

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“…Next, we confirmed that pre-inoculation of the high salt medium with homogenized salt-tolerant flies (Fs1, Fs2) improved the reproduction of control (naïve) flies on this medium compared to pre-inoculation by homogenized flies from the control lines (Fn1, Fn2). Thus we show that the results obtained previously [43, 44] are replicable in four other fly lines, reared in different conditions (see above).…”

Section: Introductionsupporting

confidence: 86%

“…We obtained this result in four laboratory lines (two salt-tolerant and two control ones) reared in population cages with overlapping generations. We also found remarkable differences in the abundance and taxonomic composition of yeasts between salt-tolerant and control Drosophila homogenates [43, 44]. These results agree with the assumption that the increased tolerance of D .…”

Section: Introductionsupporting

confidence: 84%

“…We used the same testing technique previously in the course of our experiment [25–27, 43, 44]. We performed three tests on the reproductive efficiency (sections 1, 2 and 4 of the Results); 5 to 10 test vials were used for each fly line/substrate combination.…”

Section: Methodsmentioning

confidence: 99%

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Yeasts affect tolerance of Drosophila melanogaster to food substrate with high NaCl concentration

et al. 2019

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The ability of model animal species, such as Drosophila melanogaster, to adapt quickly to various adverse conditions has been shown in many experimental evolution studies. It is usually assumed by default that such adaptation is due to changes in the gene pool of the studied population of macroorganisms. At the same time, it is known that microbiome can influence biological processes in macroorganisms. In order to assess the possible impact of microbiome on adaptation, we performed an evolutionary experiment in which some D. melanogaster lines were reared on a food substrate with high NaCl concentration while the others were reared on the standard (favourable) substrate. We evaluated the reproductive efficiency of experimental lines on the high salt substrate three years after the experiment started. Our tests confirmed that the lines reared on the salty substrate became more tolerant to high NaCl concentration. Moreover, we found that pre-inoculation of the high salt medium with homogenized salt-tolerant flies tended to improve reproductive efficiency of naïve flies on this medium (compared to pre-inoculation with homogenized control flies). The analysis of yeast microbiome in fly homogenates revealed significant differences in number and species richness of yeasts between salt-tolerant and control lines. We also found that some individual yeast lines extracted from the salt-tolerant flies improved reproductive efficiency of naïve flies on salty substrate (compared to baker’s yeast and no yeast controls), whereas the effect of the yeast lines extracted from the control flies tended to be smaller. The yeast Starmerella bacillaris extracted from the salt-tolerant flies showed the strongest positive effect. This yeast is abundant in all salt-tolerant lines, and very rare or absent in all control lines. The results are consistent with the hypothesis that some components of the yeast microbiome of D. melanogaster contribute to to flies’ tolerance to food substrate with high NaCl concentration.

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Section: Introductionsupporting

confidence: 86%

Section: Introductionsupporting

confidence: 84%

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Yeasts affect tolerance of Drosophila melanogaster to food substrate with high NaCl concentration

et al. 2019

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“…[37] Genes and metabolites that are produced by the microbiome, may provide the host with capabilities that can favor its survival under specific conditions of stress. [38][39][40][41][42][43][44] These contributions of the microbiota can be rapidly adjusted, either by changes in species composition (due to differential amplification of specific bacterial species) or by selection of variant bacteria. Owing to the large numbers of rapidly proliferating bacteria, every host encounters a large number of bacterial mutations.…”

Section: The Holobiont As a Symbiotic Organizationmentioning

confidence: 99%

“…Recent estimates further suggest that gut bacteria generate heat equivalent of about 70% of the total heat produced by an average person . Genes and metabolites that are produced by the microbiome, may provide the host with capabilities that can favor its survival under specific conditions of stress . These contributions of the microbiota can be rapidly adjusted, either by changes in species composition (due to differential amplification of specific bacterial species) or by selection of variant bacteria.…”

Section: Introductionmentioning

confidence: 99%

Microbiome‐Germline Interactions and Their Transgenerational Implications

Elgart¹,

Soen²

2017

BioEssays

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It is becoming increasingly clear that most, if not all, animals and plants are associated with a diverse array of resident gut microbiota. This symbiosis is regulated by host-microbiome interactions which influence the development, homeostasis, adaptation and evolution of the host. Recent evidence indicated that these interactions can also affect the host germline and have a potential of supporting transgenerational effects, including inheritance of acquired characteristics. Taken together, the influence of gut bacteria on the host soma and germline could potentially give rise to emergent phenotypes, which may be partially inherited by three distinguishable modes of transgenerational influence of gut bacteria: 1) "soma-to-soma" 2) "soma-to-germline" and 3) "soma-germline-soma". Here, we discuss these possibilities in light of evidence supporting bacterial-mediated modes of transgenerational inheritance.

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