Contribution of the Cell Wall Component Teichuronopeptide to pH Homeostasis and Alkaliphily in the Alkaliphile
            <i>Bacillus lentus</i>
            C-125

Aono, Rikizo; Ito, Masahiro; Machida, Takayoshi

doi:10.1128/jb.181.21.6600-6606.1999

Cited by 67 publications

(24 citation statements)

References 27 publications

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“…There is strong evidence that monovalent cation/proton antiporters are essential for maintaining a neutral cytoplasmic pH and, therefore, for growth under alkaline conditions (Krulwich, 1995). In addition, acidic cell wall polymers contribute to pH homeostasis (Aono et al, 1999). Again, how facultative alkaliphilic bacilli can cope with a sudden alkaline shock has never been examined in detail on a transcriptional level.…”

Section: Introductionmentioning

confidence: 99%

Alkaline shock induces the Bacillus subtilisσ^W regulon

Wiegert

Homuth

Versteeg

et al. 2001

Molecular Microbiology

185

205

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When confronted with a stress factor, bacteria react with a specific stress response, a genetically encoded programme resulting in the transiently enhanced expression of a subset of genes. One of these stress factors is a sudden increase in the external pH. As a first step to understand the response of Bacillus subtilis cells towards an alkali shock at the transcriptional level, we attempted to identify alkali‐inducible genes using the DNA macroarray technique. To define the appropriate challenging conditions, we used the ydjF gene, the orthologue of the Escherichia coli pspA, as a model gene for an alkali‐inducible gene. Hybridization of 33P‐labelled cDNA to a DNA macroarray revealed induction of more than 80 genes by a sudden increase in the external pH value from 6.3 to 8.9. It was discovered that a large subset of these genes belong to the recently described σW regulon, which was confirmed by the analysis of a sigW knockout. A comparison of B. subtilis wild type with the congenic sigW knockout also led to the discovery of new members of the σW regulon. In addition, we found several genes clearly not belonging to that regulon. This analysis represents the first report of an extracellular stimulus inducing the σW regulon.

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Section: Introductionmentioning

confidence: 99%

Alkaline shock induces the Bacillus subtilisσ^W regulon

Wiegert

Homuth

Versteeg

et al. 2001

Molecular Microbiology

185

205

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“…One of the reasons for the survival of these indigenous cement isolates at high pH might be their highly alkaline habitat. Reportedly, certain structural components of the cell wall of some alkalophiles, such as teichuronopeptide, may contribute to pH homeostasis at alkaline pH and aid bacteria to survive in alkaline environments [6]. Owing to the ability to tolerate high pH, CT5 enhanced the compressive strength of cement mortar cubes significantly and is regarded as a microbial concrete producer [2].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Two Urease-Producing and Calcifying Bacillus spp. Isolated from Cement

Achal

Mukherjee²,

Reddy³

2010

J. Microbiol. Biotechnol.

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Two bacterial strains designated as CT2 and CT5 were isolated from highly alkaline cement samples using the enrichment culture technique. On the basis of various physiological tests and 16S rRNA sequence analysis, the bacteria were identified as Bacillus species. The urease production was 575.87 U/ml and 670.71 U/ml for CT2 and CT5, respectively. Calcite constituted 27.6% and 31% of the total weight of sand samples plugged by CT2 and CT5, respectively. Scanning electron micrography analysis revealed the direct involvement of these isolates in calcite precipitation. This is the first report of the isolation and identification of Bacillus species from cement. Based on the ability of these bacteria to tolerate the extreme environment of cement, they have potential to be used in remediating the cracks and fissures in various building or concrete structures.

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“…With the exception of B. cereus ATCC 10702 at pH 11, results also suggested that the alkaline buffering capacity for both Bacillus DL5 and B. cereus ATCC 10702 may possibly be cell wallassociated. Reportedly, certain structural components of the cell wall of some alkalophiles, such as teichuronopeptide, may contribute to pH homeostasis at alkaline pH and aid bacteria to survive in alkaline environments (Aono et al 1999). Similar types of cell wall components may be present in Bacillus DL5 and B. cereus ATCC 10702 cells aiding in survival at alkaline pHs, although they were shown not to be alkalophiles.…”

Section: Discussionmentioning

confidence: 99%

Adaptation of a neutrophilic dairy-associated Bacillus cereus isolate to alkaline pH

et al. 2002

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Aims: This study identi®ed and studied the response of ®ve Bacillus strains, isolated from alkaline cleaning in place (CIP) solutions, to alkaline conditions. Methods and Results: Isolates were identi®ed as B. cereus by 16S rDNA sequencing. External and internal cell pH and buffering capacity data of a representative strain, Bacillus DL5, were compared to B. cereus ATCC 10702. Results indicated that a buffering system was induced when the pH of the growth medium increased to above pH 10, which was effective up to pH 12 and presumably cell wall associated. Volume measurements and confocal scanning laser microscope images of Bacillus DL5 cells showed that cells exhibited more pronounced stress symptoms when exposed to pH 10 than at pHs above 10. Long-term exposure of Bacillus DL5 to pH 10 or 10á5 indicated that cells grew in planktonic form and formed bio®lms at both pHs. Conclusions: Bacillus DL5 was a neutrophile with a growth pH range similar to B. cereus ATCC 10702, but tolerated alkaline pH. This may be a general trait of the B. cereus species rather than a speci®c phenomenon of isolates from alkaline ecosystems. Signi®cance and Impact of the Study: Other neutrophilic B. cereus isolates may exhibit similar responses to alkaline conditions as the isolates studied here. These results may have important implications for dairy manufacturers.

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Contribution of the Cell Wall Component Teichuronopeptide to pH Homeostasis and Alkaliphily in the Alkaliphile Bacillus lentus C-125

Cited by 67 publications

References 27 publications

Alkaline shock induces the Bacillus subtilisσ^W regulon

Alkaline shock induces the Bacillus subtilisσ^W regulon

Characterization of Two Urease-Producing and Calcifying Bacillus spp. Isolated from Cement

Adaptation of a neutrophilic dairy-associated Bacillus cereus isolate to alkaline pH

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Contribution of the Cell Wall Component Teichuronopeptide to pH Homeostasis and Alkaliphily in the Alkaliphile Bacillus lentus C-125

Cited by 67 publications

References 27 publications

Alkaline shock induces the Bacillus subtilisσW regulon

Alkaline shock induces the Bacillus subtilisσW regulon

Characterization of Two Urease-Producing and Calcifying Bacillus spp. Isolated from Cement

Adaptation of a neutrophilic dairy-associated Bacillus cereus isolate to alkaline pH

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Product

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Alkaline shock induces the Bacillus subtilisσ^W regulon

Alkaline shock induces the Bacillus subtilisσ^W regulon