Contribution of the coelomic epithelial cells specific to the left testis in the chicken embryo

Omotehara, Takuya; Minami, Kiichi; Mantani, Youhei; Umemura, Yuria; Nishida, Miho; Hirano, Tetsushi; Yoshioka, Hidefumi; Kitagawa, Hiroshi; Yokoyama, Toshifumi; Hoshi, Nobuhiko

doi:10.1002/dvdy.24469

Cited by 8 publications

(6 citation statements)

References 43 publications

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“…Immunohistochemistry was performed according to a previous study with some modifications for seq‐IHC . The sections were deparaffinized and hydrated in xylene, ethanol, and distilled and deionized water.…”

Section: Methodsmentioning

confidence: 99%

Connection between seminiferous tubules and epididymal duct is originally induced before sex differentiation in a sex‐independent manner

Omotehara

Kuramasu

et al. 2020

Developmental Dynamics

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Background: Spermatozoa in mammals develop in seminiferous tubules in a testis and are transported through the male reproductive tract. Their developmental origins are, however, different from each other; the seminiferous tubules are testicular (gonadal) structure but the subsequent ducts stem from the mesonephros. Although some mechanisms should function for the connection between these ducts, there are few reports on them. In the present study, basic information such as timing, localization, and cell types involved in the connection was obtained by sequential immunohistochemistry. Results: At the time when the undifferentiated gonad differentiates into the testis or ovary, Adrenal-4 binding protein/steroidogenic factor-1 (Ad4BP/SF-1)-positive gonadal cells were noted in the mesonephric tubules (MT) in both sexes. At an earlier stage, although Ad4BP/SF-1-positive coelomic epithelial cells were adjacent to the MT, a basal membrane around them was not observed. Conclusions:The connection between the testis cords and MT is suggested to be induced between Ad4BP/SF-1-positive gonadal cells and the MT before sex differentiation in a sex-independent manner. K E Y W O R D S efferent duct, male reproductive tract, sequential immunohistochemistry, testis development

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Section: Methodsmentioning

confidence: 99%

Connection between seminiferous tubules and epididymal duct is originally induced before sex differentiation in a sex‐independent manner

Omotehara

Kuramasu

et al. 2020

Developmental Dynamics

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“…A commonly used DMEM contains phenol red, which has an estrogenic effect [3]. Gonadal culture experiments using DMEM without phenol red have been reported [28]. Our gonadal culture system showed no significant difference between the use of culture medium with phenol red and that without it (Fig.…”

Section: Resultsmentioning

confidence: 64%

Establishment of an organ culture system to induce Sertoli cell differentiation from undifferentiated mouse gonads

Hasegawa

Yokoyama

Umemura

et al. 2020

The Journal of Veterinary Medical Science

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“…In chicken, the surface epithelium gives rise to non-steroidogenic interstitial cells, not the supporting cell lineage as in mouse ( Estermann et al, 2020 ; Sekido and Lovell-Badge, 2007 ). Prior to gonadal sex differentiation in chicken, the gonadal epithelial layer is thicker in the left gonad than the right gonad (in both sexes) ( Omotehara et al, 2017 ; Guioli et al, 2014 ). During sex differentiation, this asymmetry becomes less marked in males ( Guioli et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

“…Prior to gonadal sex differentiation in chicken, gonadal epithelial layer is thicker in the left gonad then the right gonad (in both sexes) (Omotehara et al, 2017, Guioli et al, 2014. During sex differentiation, this asymmetry becomes less marked in males (Guioli et al, 2014).…”

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confidence: 99%

The homeobox gene TGIF1 is required for chicken ovarian cortical development and generation of the juxtacortical medulla

et al. 2021

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During early embryogenesis in amniotic vertebrates, the gonads differentiate into either ovaries or testes. The first cell lineage to differentiate gives rise to the supporting cells; Sertoli cells in males and pre-granulosa cells in females. These key cell types direct the differentiation of the other cell types in the gonad, including steroidogenic cells. The gonadal surface epithelium and the interstitial cell populations are less well studied, and little is known about their sexual differentiation programs. Here, we show the requirement of the homeobox transcription factor gene TGIF1 for ovarian development in the chicken embryo. TGIF1 is expressed in the two principal ovarian somatic cell populations, the cortex and the pre-granulosa cells of the medulla. TGIF1 expression is associated with an ovarian phenotype in estrogen-mediated sex reversal experiments. Targeted mis-expression and gene knockdown indicate that TGIF1 is required, but not sufficient, for proper ovarian cortex formation. In addition, TGIF1 is identified as the first known regulator of juxtacortical medulla development. These findings provide new insights into chicken ovarian differentiation and development, specifically cortical and juxtacortical medulla formation.

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Contribution of the coelomic epithelial cells specific to the left testis in the chicken embryo

Abstract: Some cortical cells expressing DMRT1 were suggested to contribute to the Sertoli cells in the testis cord only after the onset of testicular development and only in the left testis. Developmental Dynamics 246:148-156, 2017. © 2016 Wiley Periodicals, Inc.

Cited by 8 publications

References 43 publications

Connection between seminiferous tubules and epididymal duct is originally induced before sex differentiation in a sex‐independent manner

Connection between seminiferous tubules and epididymal duct is originally induced before sex differentiation in a sex‐independent manner

Establishment of an organ culture system to induce Sertoli cell differentiation from undifferentiated mouse gonads

The homeobox gene TGIF1 is required for chicken ovarian cortical development and generation of the juxtacortical medulla

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