Contribution of the Endocervical Cytobrush Sample to the Diagnosis of Cervical Lesions

Luzzatto, Rui; Boon, Mathilde E.

doi:10.1159/000333972

Cited by 21 publications

(12 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2,3,7,8 Although few smears in this study were inadequate due to the absence of squamous cells, there is concern that the cytobrush may not adequately sample the squamous cells of the ectocervix and miss dysplasia or carcinoma in this region. 7,8 It is recommended that the ectocervix adjacent to the transformation zone, the transformation zone and the endocervix be sampled. 2 The most cost-effective method of sampling appears to be the combination of a cytobrush with an extended tip spatula.…”

Section: Discussionmentioning

confidence: 88%

A comparison of cervical smear adequacy using either the cytobrush or the Ayre spatula: a practice audit

O'Mahony

Banach

Igumbor

2006

South African Family Practice

View full text Add to dashboard Cite

BackgroundTo obtain an adequate cervical (Papanicolaou) smear, the transformation zone, including the squamocolumnar junction, should be sampled as carcinoma develops preferentially in this transformation zone. The Ayre spatula has been widely used but is not very effective. Other sampling devices have been developed to improve efficiency, including the cytobrush. The purpose of this study was to compare the adequacy of cervical smears taken with the Ayre spatula as opposed to the cytobrush. MethodsThis was a retrospective analytical study. One sampler, an experienced general practitioner, took the smears in the period 1990 to 2004. Initially, the Ayre spatula was used to consecutively sample the cervix and thereafter, a cytobrush alone was used. Two groups were thus formed for comparison. The presence of endocervical cells was accepted as an indicator of an adequate smear. A Cusco speculum was used to visualize the cervix. The sample was smeared onto a slide and fixated with an alcohol aerosol spray. ResultsA total of 4561 smears were taken and 247 had no endocervical cells. A further 34 smears were classified as unsatisfactory due to the presence of degeneration (19), insufficient squamous cells (7), inflammatory exudate (4), excess blood (3) and/or a thick slide (1).The cytobrush group was similar demographically to the Ayre spatula group: 1981 (99%) and 2490 (98%) respectively were non pregnant; 67 (3%) and 110 (4%) were nulliparous, 1008 (50%) and 1370 (54%) were para 1 -5, and 931 (46%) and 1075 (42%) were para 6 or more; 0 (0%) and 2 (0.1%) were aged between 10 -19 years, 1496 (75%) and 2012 (78%) between 20 -49, and 510 (25%) and 541 (21%) were aged 50 years or more. Of 2006 smears taken with a cytobrush, 1955 (97.5%) contained endocervical cells compared with 2325 (91%) of 2555 smears taken with an Ayre spatula. The difference was significant with an Odds Ratio of 4.56 (95% Confidence Interval 3.42-6.42). ConclusionThe cytobrush is significantly more efficacious than the Ayre spatula in obtaining adequate cervical smears. Use of the cytobrush will ensure less repeat smears with a consequent reduction in workload for samplers and laboratories. Although very few smears lacked sufficient squamous cells (an indicator of adequate ectocervical sampling), current best practice is that the cytobrush be used together with a wooden spatula to ensure adequate sampling of both the endocervical and ectocervical components of the transformation zone. SA Fam Pract 2006;48(9):15The full version of this article is available at: www.safpj.co.za P This article has been peer reviewed

show abstract

Section: Discussionmentioning

confidence: 88%

A comparison of cervical smear adequacy using either the cytobrush or the Ayre spatula: a practice audit

O'Mahony

Banach

Igumbor

2006

South African Family Practice

View full text Add to dashboard Cite

show abstract

“…Vários trabalhos demonstraram que a associação da escova cytobrush com a espátula de Ayre tem bom desempenho para o diagnóstico citológico da neoplasia de colo uterino 3,[8][9][10] . Luzzatto e Boon 11 analisaram separadamente a contribuição das amostras endocervical e ectocervical de cada exame para a detecção das lesões pré-neoplásicas do colo uterino. Concluíram que o diagnóstico de NIC 3 foi obtido em 98% das amostras endocervicais e em 62% das amostras ectocervicais, ao passo que 21% dos diagnósticos de NIC 1 e NIC 2 foram feitos exclusivamente a partir da amostra da ectocérvice.…”

Section: Discussionunclassified

Desempenho das Amostras do Canal Cervical e do Fundo de Saco no Diagnóstico da Neoplasia do Colo Uterino

Zeferino¹,

Catharino²,

Araujo³

et al. 2000

Rev. Bras. Ginecol. Obstet.

View full text Add to dashboard Cite

Resultados: os percentuais de exames alterados dos grupos 2 (2,6%) e 3 (2,4%), incluindo todas as lesões escamosas e glandulares, foram significativamente maiores que no grupo 1 (2%). Os percentuais de diagnóstico da lesão intra-epitelial escamosa de baixo grau (LIE-BG) não foram estatisticamente diferentes entre os três grupos (1,27; 1,25 e 1,07%). Por outro lado, os percentuais de diagnóstico da lesão intra-epitelial escamosa de alto grau (LIE-AG) foram significativamente maiores nos grupos 2 (0,81%) e 3 (0,77%) do que no grupo 1 (0,54%

show abstract

“…1 ). Dies ist ein wichtiger Aspekt bei der Bewertung des ThinPrep-Verfahrens, denn das Vorhandensein endozervikaler Zellen gilt als Marker für eine ausreichende Repräsentativität der Zellprobe und ist mit der Zuverlässigkeit des Nachweises von Dysplasien korreliert, da dysplastische Zellveränderungen im Bereich der Transformationszone entstehen, welche ganz oder teilweise innerhalb des Zervikalkanals liegen kann [13,14] . Diese Überlegung ist jedoch nicht unumstritten.…”

Section: Comparaison Du Frottis De Dépistage Cervical Conventionnel Aunclassified

Vergleich der ThinPrep®-Dünnschichtzytologie mit der konventionellen zervikalen Exfoliativzytologie in einem Risikokollektiv unter Verwendung der Münchener Nomenklatur II

Lellé

Cordes

Regidor

et al. 2007

Gynäkol Geburtshilfliche Rundsch

View full text Add to dashboard Cite

Contribution of the Endocervical Cytobrush Sample to the Diagnosis of Cervical Lesions

Cited by 21 publications

References 6 publications

A comparison of cervical smear adequacy using either the cytobrush or the Ayre spatula: a practice audit

A comparison of cervical smear adequacy using either the cytobrush or the Ayre spatula: a practice audit

Desempenho das Amostras do Canal Cervical e do Fundo de Saco no Diagnóstico da Neoplasia do Colo Uterino

Vergleich der ThinPrep®-Dünnschichtzytologie mit der konventionellen zervikalen Exfoliativzytologie in einem Risikokollektiv unter Verwendung der Münchener Nomenklatur II

Contact Info

Product

Resources

About