Contribution of the murI Gene Encoding Glutamate Racemase in the Motility and Virulence of Ralstonia solanacearum

Choi, Kihyuck; Son, Geun Ju; Ahmad, Shabir; Lee, Seon-Woo; Lee, Hyoung Ju

doi:10.5423/ppj.oa.03.2020.0049

Cited by 5 publications

(3 citation statements)

References 37 publications

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“…MurI is a glutamate racemase that converts L-glutamate to D-glutamate, which is incorporated into peptide cross-linkages in peptidoglycan. The murI gene is required for multiple Ralstonia strains to grow in tomato stems (23,24). For all three study strains, other xylem sap fitness factors included tatC (involved in Tat-dependent protein secretion), dsbA which catalyzes disulfide bonds in periplasmic proteins, and a gene encoding a lipoprotein of unknown function (RSc2940, RSc0285, and RSc2007, respectively).…”

Section: Resultsmentioning

confidence: 99%

Genome-Wide Identification of Tomato Xylem Sap Fitness Factors for Three Plant-Pathogenic Ralstonia Species

et al. 2021

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Section: Resultsmentioning

confidence: 99%

Genome-Wide Identification of Tomato Xylem Sap Fitness Factors for Three Plant-Pathogenic Ralstonia Species

et al. 2021

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“…MurI is a glutamate racemase that converts L-Glutamate to D-Glutamate, which is incorporated into peptide cross-linkages in peptidoglycan. The murI gene is required for multiple Ralstonia strains to grow in tomato stems (23,24). For all three study strains, other xylem sap fitness factors included tatC (involved in Tat-dependent protein secretion), dsbA which catalyzes disulfide bonds in periplasmic proteins, and a lipoprotein of unknown function (RSc2940, RSc0285, and RSc2007, respectively).…”

Section: Rb-tnseq Experiments Identify Mutants With Altered Fitness In Xylem Sap Andmentioning

confidence: 99%

Genome-wide identification of tomato xylem sap fitness factors for three plant-pathogenicRalstoniaspecies

Georgoulis

Shalvarjian

Helmann

et al. 2020

Preprint

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Plant pathogenic Ralstonia spp. colonize plant xylem and cause wilt diseases on a broad range of host plants. To identify genes that promote growth of diverse Ralstonia strains in xylem sap from tomato plants, we performed genome-scale genetic screens (random barcoded transposon mutant sequencing screens; RB-TnSeq) in Ralstonia pseudosolanacearum GMI1000 and R. syzygii PSI07. Contrasting mutant fitness phenotypes in culture media versus in xylem sap suggest that Ralstonia strains are adapted to sap and that culture media impose foreign selective pressures. Although wild-type Ralstonia grew in sap and in rich medium with similar doubling times and to a similar carrying capacity, more genes were essential for growth in sap than in rich medium. Multiple mutants lacking amino acid biosynthesis and central metabolism functions had fitness defects in xylem sap and minimal medium. Our screen identified > 26 genes in each strain that contributed to growth in xylem sap but were dispensable for growth in culture media. Many sap-specific fitness factors are associated with bacterial stress responses: envelope remodeling and repair processes such as peptidoglycan peptide formation (murI and RSc1177), LPS O-antigen biosynthesis (RSc0684), periplasmic protein folding (dsbA), drug efflux (tolA and tolR), and stress responses (cspD3). Our genome-scale genetic screen identified Ralstonia fitness factors that promote growth in xylem sap, an ecologically relevant condition.ImportanceTraditional transposon mutagenesis genetic screens pioneered molecular plant pathology and identified core virulence traits like the type III secretion system. TnSeq approaches that leverage next-generation sequencing to rapidly quantify transposon mutant phenotypes are ushering in a new wave of biological discovery. Here we have adapted a genome-scale approach, random barcoded transposon mutant sequencing (RB-TnSeq), to discover fitness factors that promote growth of two related bacterial strains in a common niche, tomato xylem sap. Fitness of wild-type and mutants show that Ralstonia spp. are adapted to grow well in xylem sap from their natural host plant, tomato. Our screen identified multiple sap-specific fitness factors with roles in maintaining the bacterial envelope. These factors are putative adaptations to resist plant defenses, including antimicrobial proteins and specialized metabolites that damage bacterial membranes.

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“…According to the different genes contained, GI can be generally divided into virulence islands, drug resistance islands, metabolic islands, symbiotic islands ( Shrivastava et al, 2010 ). Then gene islands, secreted proteins are generally considered when identifying virulence factors, which play a key role in enhancing the pathogenic efficacy of pathogens ( Stritzler et al, 2018 ; Choi et al, 2020 ). The NCBI database has published the complete draft genome of 145 R. solanacearum .…”

Section: Introductionmentioning

confidence: 99%

Comprehensive genome sequence analysis of Ralstonia solanacearum gd-2, a phylotype I sequevar 15 strain collected from a tobacco bacterial phytopathogen

Xiao,

Li,

Yang

et al. 2024

Front. Microbiol.

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IntroductionPlant bacterial wilt is an important worldwide disease caused by Ralstonia solanacearum which is a complex of species.MethodsIn this study, we identified and sequenced the genome of R. solanacearum strain gd-2 isolated from tobacco.ResultsStrain gd-2 was identified as R. solanacearum species complex (RSSC) phylotype I sequevar 15 and exhibited strong pathogenicity to tobacco. The genome size of gd-2 was 5.93 Mb, including the chromosomes (3.83 Mb) and the megaplasmid (2.10 Mb). Gene prediction results showed that 3,434 and 1,640 genes were identified in the chromosomes and plasmids, respectively. Comparative genomic analysis showed that gd-2 exhibited high conservation with ten highly similar strain genomes and the differences between gd-2 and other genomes were mainly located at positions GI12-GI14. 72 type III effectors (T3Es) were identified and RipAZ2 was a T3E specific to gd-2 compared with other eight sequenced strain.DiscussionOur study provides a new basis and evidence for studying the pathogenic mechanism of R. solanacearum.

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Contribution of the murI Gene Encoding Glutamate Racemase in the Motility and Virulence of Ralstonia solanacearum

Cited by 5 publications

References 37 publications

Genome-Wide Identification of Tomato Xylem Sap Fitness Factors for Three Plant-Pathogenic Ralstonia Species

Genome-Wide Identification of Tomato Xylem Sap Fitness Factors for Three Plant-Pathogenic Ralstonia Species

Genome-wide identification of tomato xylem sap fitness factors for three plant-pathogenicRalstoniaspecies

Comprehensive genome sequence analysis of Ralstonia solanacearum gd-2, a phylotype I sequevar 15 strain collected from a tobacco bacterial phytopathogen

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