Contribution of the Wolffian duct mesenchyme to the formation of the female reproductive tract

Zhao, Fei; Grimm, Sara A.; Jia, Shuai; Yao, Hisayuki

doi:10.1093/pnasnexus/pgac182

Cited by 7 publications

(9 citation statements)

References 94 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported that Igf1 is enriched in the cranial MD (future oviduct). 20 Our Tcf21 RNAscope staining confirmed the enriched expression of Tcf21 in the mesothelial layer of the uterus but not in the oviduct (Figure 2C). These results validated F I G U R E 5 Gene expression comparison between oviductal and uterine pericytes.…”

Section: Pericytes and Mesothelial Cellssupporting

confidence: 53%

“…The manufacturer's protocol was followed as previously described. 20 Briefly, paraffin sections were treated with antigen retrieval buffer and proteinase. Sections were hybridized with the following individual probes (Angptl7, Cat #: 552821; Wfdc1, Cat #: 471331; Tcf21, Cat #: 508661; Nefm, Cat #: 315611; Gsap, Cat #: 318661) and subjected to rinsing and signaling detection at designated temperatures.…”

Section: Rna-scopementioning

confidence: 99%

See 1 more Smart Citation

Single‐cell transcriptomic profiling of the neonatal oviduct and uterus reveals new insights into upper Müllerian duct regionalization

Jia,

Zhao

2024

The FASEB Journal

Self Cite

View full text Add to dashboard Cite

The upper Müllerian duct (MD) is patterned and specified into two morphologically and functionally distinct organs, the oviduct and uterus. It is known that this regionalization process is instructed by inductive signals from the adjacent mesenchyme. However, the interaction landscape between epithelium and mesenchyme during upper MD development remains largely unknown. Here, we performed single‐cell transcriptomic profiling of mouse neonatal oviducts and uteri at the initiation of MD epithelial differentiation (postnatal day 3). We identified major cell types including epithelium, mesenchyme, pericytes, mesothelium, endothelium, and immune cells in both organs with established markers. Moreover, we uncovered region‐specific epithelial and mesenchymal subpopulations and then deduced region‐specific ligand‐receptor pairs mediating mesenchymal‐epithelial interactions along the craniocaudal axis. Unexpectedly, we discovered a mesenchymal subpopulation marked by neurofilaments with specific localizations at the mesometrial pole of both the neonatal oviduct and uterus. Lastly, we analyzed and revealed organ‐specific signature genes of pericytes and mesothelial cells. Taken together, our study enriches our knowledge of upper MD development, and provides a manageable list of potential genes, pathways, and region‐specific cell subtypes for future functional studies.

show abstract

Section: Pericytes and Mesothelial Cellssupporting

confidence: 53%

Section: Rna-scopementioning

confidence: 99%

Single‐cell transcriptomic profiling of the neonatal oviduct and uterus reveals new insights into upper Müllerian duct regionalization

Jia,

Zhao

2024

The FASEB Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…6B ). It has been reported that Igf1 is enriched in the cranial MD (future oviduct) (56). Our Tcf21 RNAscope staining confirmed the enriched expression of Tcf21 in the mesothelial layer of the uterus but not in the oviduct ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…It has been noticed that the neural crest cells invade into the mesometrial pole of the Müllerian duct (79). Also, during sexual differentiation, Wolffian ducts degenerate in females however the mesenchyme surrounding Wolffian ducts is maintained and contributes to mesenchymal cells at the mesometrial side of the uterus (56). These observations suggest that Nefm+ mesenchymal subpopulation might derive from neural crest cells and/or Wolffian duct mesenchyme.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Single-cell transcriptomic profiling of the neonatal oviduct and uterus reveals new insights into upper Müllerian duct regionalization

Jia,

Zhao

2023

Preprint

Self Cite

View full text Add to dashboard Cite

The upper Müllerian duct (MD) is patterned and specified into two morphologically and functionally distinct organs, the oviduct and uterus. It is known that this regionalization process is instructed by inductive signals from the adjacent mesenchyme. However, the interaction landscape between epithelium and mesenchyme during upper MD development remains largely unknown. Here, we performed single-cell transcriptomic profiling of mouse neonatal oviducts and uteri at the initiation of MD epithelial differentiation (postnatal day 3). We identified major cell types including epithelium, mesenchyme, pericytes, mesothelium, endothelium, and immune cells in both organs with established markers. Moreover, we uncovered region-specific epithelial and mesenchymal subpopulations and then deduced region-specific ligand-receptor pairs mediating mesenchymal-epithelial interactions along the craniocaudal axis. Unexpectedly, we discovered a mesenchymal subpopulation marked by neurofilaments with specific localizations at the mesometrial pole of both the neonatal oviduct and uterus. Lastly, we analyzed and revealed organ-specific signature genes of pericytes and mesothelial cells. Taken together, our study enriches our knowledge of upper Müllerian duct development, and provides a manageable list of potential genes, pathways, and region-specific cell subtypes for future functional studies.

show abstract

SMAD2/3 signaling regulates initiation of mouse Wolffian ducts and proximal differentiation in Müllerian ducts

Nakajima,

Imai,

Ishii

et al. 2023

FEBS Open Bio

View full text Add to dashboard Cite

Male and female reproductive tracts develop from anterior intermediate mesoderm with similar differentiation processes. The anterior intermediate mesoderm develops into the mesonephros, and the Wolffian duct initiates by epithelialization in the mesonephros. The Müllerian duct invaginates from the coelomic epithelium of the cranial mesonephros for ductal formation and is then regionalized into proximal to caudal female reproductive tracts. In this study, we focused on the epithelialization of the Wolffian duct, initiation of the Müllerian duct, and the regionalization step of the Müllerian ducts as a continuous process. By using intermediate mesodermal cells from mouse pluripotent stem cells, we identified that inhibition of SMAD2/3 signaling might be involved in the differentiation into mesenchymal cells, after which mesonephric cells might be then epithelialized during differentiation of the Wolffian duct. Aggregation of coelomic epithelial cells might be related to initiation of the Müllerian duct. Transcriptomic analysis predicted that consensus sequences of SMAD3/4 were enriched among highly expressed genes in the proximal Müllerian duct. SMAD2/3 signaling to regulate differentiation of the Wolffian duct was continuously activated in the proximal Müllerian duct and was involved in proximal and oviductal regionalization. Therefore, SMAD2/3 signaling may be finely tuned to regulate differentiation from initiation to regionalization steps.

show abstract

Contribution of the Wolffian duct mesenchyme to the formation of the female reproductive tract

Cited by 7 publications

References 94 publications

Single‐cell transcriptomic profiling of the neonatal oviduct and uterus reveals new insights into upper Müllerian duct regionalization

Single‐cell transcriptomic profiling of the neonatal oviduct and uterus reveals new insights into upper Müllerian duct regionalization

Single-cell transcriptomic profiling of the neonatal oviduct and uterus reveals new insights into upper Müllerian duct regionalization

SMAD2/3 signaling regulates initiation of mouse Wolffian ducts and proximal differentiation in Müllerian ducts

Contact Info

Product

Resources

About